Antibody-Based Protective Immunity against Helminth Infections: Antibody Phage Display Derived Antibodies against BmR1 Antigen
Helminth parasite infections are significantly impacting global health, with more than two billion infections worldwide with a high morbidity rate. The complex life cycle of the nematodes has made host immune response studies against these parasites extremely difficult. In this study, we utilized...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI
2017
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| Online Access: | http://eprints.usm.my/38329/1/Antibody-Based_Protective_Immunity_against_Helminth_Infections.pdf |
| _version_ | 1797010010442563584 |
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| author | Rahumatullah, Anizah Abdul Karim, Izzati Zahidah Noordin, Rahmah Theam, Soon Lim |
| author_facet | Rahumatullah, Anizah Abdul Karim, Izzati Zahidah Noordin, Rahmah Theam, Soon Lim |
| author_sort | Rahumatullah, Anizah |
| collection | USM |
| description | Helminth parasite infections are significantly impacting global health, with more than two
billion infections worldwide with a high morbidity rate. The complex life cycle of the nematodes
has made host immune response studies against these parasites extremely difficult. In this study,
we utilized two phage antibody libraries; the immune and naïve library were used to identify single
chain fragment variable (scFv) clones against a specific filarial antigen (BmR1). The V-gene analysis of
isolated scFv clones will help shed light on preferential VDJ gene segment usage against the filarial
BmR1 antigen in healthy and infected states. The immune library showed the usage of both lambda
and kappa light chains. However, the naïve library showed preferential use of the lambda family
with different amino acid distributions. The binding characteristics of the scFv clones identified
from this work were analyzed by immunoassay and immunoaffinity pull down of BmR1. The work
highlights the antibody gene usage pattern of a naïve and immune antibody library against the same
antigen as well as the robust nature of the enriched antibodies for downstream applications. |
| first_indexed | 2024-03-06T15:13:41Z |
| format | Article |
| id | usm.eprints-38329 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T15:13:41Z |
| publishDate | 2017 |
| publisher | MDPI |
| record_format | dspace |
| spelling | usm.eprints-383292018-01-10T07:21:40Z http://eprints.usm.my/38329/ Antibody-Based Protective Immunity against Helminth Infections: Antibody Phage Display Derived Antibodies against BmR1 Antigen Rahumatullah, Anizah Abdul Karim, Izzati Zahidah Noordin, Rahmah Theam, Soon Lim R735-854 Medical education. Medical schools. Research Helminth parasite infections are significantly impacting global health, with more than two billion infections worldwide with a high morbidity rate. The complex life cycle of the nematodes has made host immune response studies against these parasites extremely difficult. In this study, we utilized two phage antibody libraries; the immune and naïve library were used to identify single chain fragment variable (scFv) clones against a specific filarial antigen (BmR1). The V-gene analysis of isolated scFv clones will help shed light on preferential VDJ gene segment usage against the filarial BmR1 antigen in healthy and infected states. The immune library showed the usage of both lambda and kappa light chains. However, the naïve library showed preferential use of the lambda family with different amino acid distributions. The binding characteristics of the scFv clones identified from this work were analyzed by immunoassay and immunoaffinity pull down of BmR1. The work highlights the antibody gene usage pattern of a naïve and immune antibody library against the same antigen as well as the robust nature of the enriched antibodies for downstream applications. MDPI 2017 Article PeerReviewed application/pdf en http://eprints.usm.my/38329/1/Antibody-Based_Protective_Immunity_against_Helminth_Infections.pdf Rahumatullah, Anizah and Abdul Karim, Izzati Zahidah and Noordin, Rahmah and Theam, Soon Lim (2017) Antibody-Based Protective Immunity against Helminth Infections: Antibody Phage Display Derived Antibodies against BmR1 Antigen. International Journal of Molecular Sciences, 18 (11). pp. 1-21. ISSN 1422-0067 http://www.mdpi.com/1422-0067/18/11/2376 |
| spellingShingle | R735-854 Medical education. Medical schools. Research Rahumatullah, Anizah Abdul Karim, Izzati Zahidah Noordin, Rahmah Theam, Soon Lim Antibody-Based Protective Immunity against Helminth Infections: Antibody Phage Display Derived Antibodies against BmR1 Antigen |
| title | Antibody-Based Protective Immunity against Helminth Infections: Antibody Phage Display Derived Antibodies against BmR1 Antigen |
| title_full | Antibody-Based Protective Immunity against Helminth Infections: Antibody Phage Display Derived Antibodies against BmR1 Antigen |
| title_fullStr | Antibody-Based Protective Immunity against Helminth Infections: Antibody Phage Display Derived Antibodies against BmR1 Antigen |
| title_full_unstemmed | Antibody-Based Protective Immunity against Helminth Infections: Antibody Phage Display Derived Antibodies against BmR1 Antigen |
| title_short | Antibody-Based Protective Immunity against Helminth Infections: Antibody Phage Display Derived Antibodies against BmR1 Antigen |
| title_sort | antibody based protective immunity against helminth infections antibody phage display derived antibodies against bmr1 antigen |
| topic | R735-854 Medical education. Medical schools. Research |
| url | http://eprints.usm.my/38329/1/Antibody-Based_Protective_Immunity_against_Helminth_Infections.pdf |
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