Antimicrobial Activity of Artemisinin and Precursor Derived from In Vitro Plantlets of Artemisia annua L.
Artemisia annua L., a medicinal herb, produces secondary metabolites with antimicrobial property. InMalaysia due to the tropical hot climate, A. annua could not be planted for production of artemisinin, themain bioactive compound. In this study, the leaves of three in vitro A. annua L. clones were...
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| Format: | Article |
| Language: | English |
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Hindawi Publishing Corporation
2014
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| Subjects: | |
| Online Access: | http://eprints.usm.my/38335/1/Antimicrobial_Activity_of_Artemisinin_and_Precursor_Derived_from_In_Vitro_Plantlets_of.pdf |
| _version_ | 1797010011689320448 |
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| author | Appalasamy, Suganthi Kiah, Yann Lo Song, Jin Ch’ng Ku Nornadia, Ku Nornadia Othman, Ahmad Sofiman Chan, Lai Keng |
| author_facet | Appalasamy, Suganthi Kiah, Yann Lo Song, Jin Ch’ng Ku Nornadia, Ku Nornadia Othman, Ahmad Sofiman Chan, Lai Keng |
| author_sort | Appalasamy, Suganthi |
| collection | USM |
| description | Artemisia annua L., a medicinal herb, produces secondary metabolites with antimicrobial property. InMalaysia due to the tropical
hot climate, A. annua could not be planted for production of artemisinin, themain bioactive compound. In this study, the leaves of
three in vitro A. annua L. clones were, extracted and two bioactive compounds, artemisinin and a precursor, were isolated by thin
layer chromatography. These compounds were found to be effective in inhibiting the growth of Gram-positive and Gram-negative
bacteria but not Candida albicans.Their antimicrobial activity was similar to that of antibactericidal antibiotic streptomycin. They
were found to inhibit the growth of the tested microbes at the minimum inhibition concentration of 0.09mg/mL, and toxicity test
using brine shrimp showed that even the low concentration of 0.09mg/mL was very lethal towards the brine shrimps with 100%
mortality rate. This study hence indicated that in vitro cultured plantlets of A. annua can be used as the alternative method for
production of artemisinin and its precursor with antimicrobial activities. |
| first_indexed | 2024-03-06T15:13:42Z |
| format | Article |
| id | usm.eprints-38335 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T15:13:42Z |
| publishDate | 2014 |
| publisher | Hindawi Publishing Corporation |
| record_format | dspace |
| spelling | usm.eprints-383352018-01-11T00:50:18Z http://eprints.usm.my/38335/ Antimicrobial Activity of Artemisinin and Precursor Derived from In Vitro Plantlets of Artemisia annua L. Appalasamy, Suganthi Kiah, Yann Lo Song, Jin Ch’ng Ku Nornadia, Ku Nornadia Othman, Ahmad Sofiman Chan, Lai Keng QH1 Natural history (General - Including nature conservation, geographical distribution) Artemisia annua L., a medicinal herb, produces secondary metabolites with antimicrobial property. InMalaysia due to the tropical hot climate, A. annua could not be planted for production of artemisinin, themain bioactive compound. In this study, the leaves of three in vitro A. annua L. clones were, extracted and two bioactive compounds, artemisinin and a precursor, were isolated by thin layer chromatography. These compounds were found to be effective in inhibiting the growth of Gram-positive and Gram-negative bacteria but not Candida albicans.Their antimicrobial activity was similar to that of antibactericidal antibiotic streptomycin. They were found to inhibit the growth of the tested microbes at the minimum inhibition concentration of 0.09mg/mL, and toxicity test using brine shrimp showed that even the low concentration of 0.09mg/mL was very lethal towards the brine shrimps with 100% mortality rate. This study hence indicated that in vitro cultured plantlets of A. annua can be used as the alternative method for production of artemisinin and its precursor with antimicrobial activities. Hindawi Publishing Corporation 2014 Article PeerReviewed application/pdf en http://eprints.usm.my/38335/1/Antimicrobial_Activity_of_Artemisinin_and_Precursor_Derived_from_In_Vitro_Plantlets_of.pdf Appalasamy, Suganthi and Kiah, Yann Lo and Song, Jin Ch’ng and Ku Nornadia, Ku Nornadia and Othman, Ahmad Sofiman and Chan, Lai Keng (2014) Antimicrobial Activity of Artemisinin and Precursor Derived from In Vitro Plantlets of Artemisia annua L. BioMed Research International, 2014 (215872). pp. 1-6. ISSN 2314-6133 http://dx.doi.org/10.1155/2014/215872 |
| spellingShingle | QH1 Natural history (General - Including nature conservation, geographical distribution) Appalasamy, Suganthi Kiah, Yann Lo Song, Jin Ch’ng Ku Nornadia, Ku Nornadia Othman, Ahmad Sofiman Chan, Lai Keng Antimicrobial Activity of Artemisinin and Precursor Derived from In Vitro Plantlets of Artemisia annua L. |
| title | Antimicrobial Activity of Artemisinin and Precursor Derived
from In Vitro Plantlets of Artemisia annua L. |
| title_full | Antimicrobial Activity of Artemisinin and Precursor Derived
from In Vitro Plantlets of Artemisia annua L. |
| title_fullStr | Antimicrobial Activity of Artemisinin and Precursor Derived
from In Vitro Plantlets of Artemisia annua L. |
| title_full_unstemmed | Antimicrobial Activity of Artemisinin and Precursor Derived
from In Vitro Plantlets of Artemisia annua L. |
| title_short | Antimicrobial Activity of Artemisinin and Precursor Derived
from In Vitro Plantlets of Artemisia annua L. |
| title_sort | antimicrobial activity of artemisinin and precursor derived from in vitro plantlets of artemisia annua l |
| topic | QH1 Natural history (General - Including nature conservation, geographical distribution) |
| url | http://eprints.usm.my/38335/1/Antimicrobial_Activity_of_Artemisinin_and_Precursor_Derived_from_In_Vitro_Plantlets_of.pdf |
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