Antioxidant Activity and Hepatoprotective Potential of Polyalthia longifolia and Cassia spectabilis Leaves against Paracetamol-Induced Liver Injury
In the present study, in vitro antioxidant, free radical scavenging capacity, and hepatoprotective activity of methanol extracts from Polyalthia longifolia and Cassia spectabilis were evaluated using established in vitro models such as ferric-reducing antioxidant power (FRAP), 2,2-diphenyl-1-picry...
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| Format: | Article |
| Language: | English |
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Hindawi Publishing Corporation
2012
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| Online Access: | http://eprints.usm.my/38337/1/Antioxidant_Activity_and_Hepatoprotective_Potential_of_Polyalthia_longifolia_and_Cassia.pdf |
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| author | L. Jothy, Subramanion Aziz, Azlan Yeng, Chen Sasidharan, Sreenivasan |
| author_facet | L. Jothy, Subramanion Aziz, Azlan Yeng, Chen Sasidharan, Sreenivasan |
| author_sort | L. Jothy, Subramanion |
| collection | USM |
| description | In the present study, in vitro antioxidant, free radical scavenging capacity, and hepatoprotective activity of methanol extracts from
Polyalthia longifolia and Cassia spectabilis were evaluated using established in vitro models such as ferric-reducing antioxidant
power (FRAP), 2,2-diphenyl-1-picryl-hydrazyl (DPPH•), hydroxyl radical (OH•), nitric oxide radical (NO•) scavenging, metal
chelating, and antilipidperoxidation activities. Interestingly, all the extracts showed considerable in vitro antioxidant and free
radical scavenging activities in a dose-dependent manner when compared to the standard antioxidant which verified the presence
of strong antioxidant compound in leaf extracts tested. Phenolic and flavonoid content of these extracts is significantly correlated
with antioxidant capacity. Since P. longifolia extract was exhibited better in vitro antioxidant activities, it was subjected for in
vivo hepatoprotective activity in paracetamol-intoxicated mice. Therapy of P. longifolia showed the liver protective effect on
biochemical and histopathological alterations. Moreover, histological studies also supported the biochemical finding, that is, the
maximum improvement in the histoarchitecture of the liver. Results revealed that P. longifolia leaf extract could protect the liver
against paracetamol-induced oxidative damage by possibly increasing the antioxidant protection mechanism inmice. Our findings
indicated that P. longifolia and C. spectabilis have potential as good sources of natural antioxidant/antiaging compounds. |
| first_indexed | 2024-03-06T15:13:43Z |
| format | Article |
| id | usm.eprints-38337 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T15:13:43Z |
| publishDate | 2012 |
| publisher | Hindawi Publishing Corporation |
| record_format | dspace |
| spelling | usm.eprints-383372018-01-11T01:47:51Z http://eprints.usm.my/38337/ Antioxidant Activity and Hepatoprotective Potential of Polyalthia longifolia and Cassia spectabilis Leaves against Paracetamol-Induced Liver Injury L. Jothy, Subramanion Aziz, Azlan Yeng, Chen Sasidharan, Sreenivasan R735-854 Medical education. Medical schools. Research In the present study, in vitro antioxidant, free radical scavenging capacity, and hepatoprotective activity of methanol extracts from Polyalthia longifolia and Cassia spectabilis were evaluated using established in vitro models such as ferric-reducing antioxidant power (FRAP), 2,2-diphenyl-1-picryl-hydrazyl (DPPH•), hydroxyl radical (OH•), nitric oxide radical (NO•) scavenging, metal chelating, and antilipidperoxidation activities. Interestingly, all the extracts showed considerable in vitro antioxidant and free radical scavenging activities in a dose-dependent manner when compared to the standard antioxidant which verified the presence of strong antioxidant compound in leaf extracts tested. Phenolic and flavonoid content of these extracts is significantly correlated with antioxidant capacity. Since P. longifolia extract was exhibited better in vitro antioxidant activities, it was subjected for in vivo hepatoprotective activity in paracetamol-intoxicated mice. Therapy of P. longifolia showed the liver protective effect on biochemical and histopathological alterations. Moreover, histological studies also supported the biochemical finding, that is, the maximum improvement in the histoarchitecture of the liver. Results revealed that P. longifolia leaf extract could protect the liver against paracetamol-induced oxidative damage by possibly increasing the antioxidant protection mechanism inmice. Our findings indicated that P. longifolia and C. spectabilis have potential as good sources of natural antioxidant/antiaging compounds. Hindawi Publishing Corporation 2012 Article PeerReviewed application/pdf en http://eprints.usm.my/38337/1/Antioxidant_Activity_and_Hepatoprotective_Potential_of_Polyalthia_longifolia_and_Cassia.pdf L. Jothy, Subramanion and Aziz, Azlan and Yeng, Chen and Sasidharan, Sreenivasan (2012) Antioxidant Activity and Hepatoprotective Potential of Polyalthia longifolia and Cassia spectabilis Leaves against Paracetamol-Induced Liver Injury. Evidence-Based Complementary and Alternative Medicine, 2012 (561284). pp. 1-10. ISSN 1741-427X http://dx.doi.org/10.1155/2012/561284 |
| spellingShingle | R735-854 Medical education. Medical schools. Research L. Jothy, Subramanion Aziz, Azlan Yeng, Chen Sasidharan, Sreenivasan Antioxidant Activity and Hepatoprotective Potential of Polyalthia longifolia and Cassia spectabilis Leaves against Paracetamol-Induced Liver Injury |
| title | Antioxidant Activity and Hepatoprotective Potential of
Polyalthia longifolia and Cassia spectabilis Leaves against
Paracetamol-Induced Liver Injury |
| title_full | Antioxidant Activity and Hepatoprotective Potential of
Polyalthia longifolia and Cassia spectabilis Leaves against
Paracetamol-Induced Liver Injury |
| title_fullStr | Antioxidant Activity and Hepatoprotective Potential of
Polyalthia longifolia and Cassia spectabilis Leaves against
Paracetamol-Induced Liver Injury |
| title_full_unstemmed | Antioxidant Activity and Hepatoprotective Potential of
Polyalthia longifolia and Cassia spectabilis Leaves against
Paracetamol-Induced Liver Injury |
| title_short | Antioxidant Activity and Hepatoprotective Potential of
Polyalthia longifolia and Cassia spectabilis Leaves against
Paracetamol-Induced Liver Injury |
| title_sort | antioxidant activity and hepatoprotective potential of polyalthia longifolia and cassia spectabilis leaves against paracetamol induced liver injury |
| topic | R735-854 Medical education. Medical schools. Research |
| url | http://eprints.usm.my/38337/1/Antioxidant_Activity_and_Hepatoprotective_Potential_of_Polyalthia_longifolia_and_Cassia.pdf |
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