| Summary: | The present study evaluated the antioxidant activity and potential toxicity of 50% methanolic extract of Orthosiphon stamineus
(Lamiaceae) leaves (MEOS) after acute and subchronic administration in rats. Superoxide radical scavenging, hydroxyl radical
scavenging, and ferrous ion chelating methods were used to evaluate the antioxidant properties of the extract. In acute toxicity
study, single dose of MEOS, 5000mg/kg, was administered to rats by oral gavage, and the treated rats were monitored for 14
days. While in the subchronic toxicity study, MEOS was administered orally, at doses of 1250, 2500, and 5000mg/kg/day for 28
days. From the results, MEOS showed good superoxide radical scavenging, hydroxyl radical scavenging, ferrous ion chelating,
and antilipid peroxidation activities. There was no mortality detected or any signs of toxicity in acute and subchronic toxicity
studies. Furthermore, there was no significant difference in bodyweight, relative organ weight, and haematological and biochemical
parameters between bothmale and female treated rats in any doses tested.No abnormality of internal organs was observed between
treatment and control groups.Theoral lethal dose determined wasmore than 5000mg/kg and the no-observed-adverse-effect level
(NOAEL) of MEOS for both male and female rats is considered to be 5000mg/kg per day.
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