Association of anti-CLIC2 and anti-HMGB1 autoantibodies with higher disease activity in systemic lupus erythematosus patients
Systemic lupus erythematosus (SLE) is a systemic autoimmune disease characterized by numerous autoantibodies. In this study, we investigated the presence of anti-chloride intracellular channel 2 (anti-CLIC2) and anti-high mobility group box 1 (anti-HMGB1) autoantibodies in SLE patients (n = 43) ve...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Medknow Publications
2017
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| Online Access: | http://eprints.usm.my/38371/1/Association_of_anti-CLIC2_and_anti-HMGB1_autoantibodies.pdf |
| _version_ | 1797010018937077760 |
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| author | CS, Syahidatulamali WG, Wan Syamimee Y, Nor Azwany Wong, KK CH, Che Maraina |
| author_facet | CS, Syahidatulamali WG, Wan Syamimee Y, Nor Azwany Wong, KK CH, Che Maraina |
| author_sort | CS, Syahidatulamali |
| collection | USM |
| description | Systemic lupus erythematosus (SLE) is a systemic autoimmune disease characterized by
numerous autoantibodies. In this study, we investigated the presence of anti-chloride intracellular channel 2
(anti-CLIC2) and anti-high mobility group box 1 (anti-HMGB1) autoantibodies in SLE patients (n = 43) versus
healthy controls ([HCs] n = 43), and their association with serological parameters (antinuclear antibody [ANA],
anti-double-stranded DNA [anti-dsDNA], and C-reactive protein [CRP]) and disease activity using Systemic
Lupus Erythematosus Disease Activity Index (SLEDAI) score (active or inactive). Settings and Design: Case–
control study at Rheumatology Clinic of Universiti Sains Malaysia Hospital. Subjects and Methods: The sera of
SLE patients and HCs were tested for the presence of anti-CLIC2 and anti-HMGB1 autoantibodies using human
recombinant proteins and ELISA methodologies. Other serological parameters were evaluated according to
routine procedures, and patients' demographic and clinical data were obtained. Statistical Analysis: Mann–
Whitney U-test, Chi-square test, Fisher's exact test, and receiver operating characteristic analysis. Results:
Anti-CLIC2 autoantibody levels were significantly higher in SLE patients compared to HCs (P = 0.0035), whereas
anti-HMGB1 autoantibody levels were not significantly elevated (P = 0.7702). Anti-CLIC2 and anti-HMGB1
autoantibody levels were not associated with ANA pattern, anti-dsDNA, and CRP. Interestingly, SLEDAI score
(≥6) was associated with anti-CLIC2 (P = 0.0046) and with anti-HMGB1 (P = 0.0091) autoantibody levels.
Conclusion: Our findings support the potential of using anti-CLIC2 autoantibodies as a novel biomarker for SLE
patients. Both anti-CLIC2 and anti-HMGB1 autoantibody levels demonstrated potential in monitoring SLE disease
activity. |
| first_indexed | 2024-03-06T15:13:49Z |
| format | Article |
| id | usm.eprints-38371 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T15:13:49Z |
| publishDate | 2017 |
| publisher | Medknow Publications |
| record_format | dspace |
| spelling | usm.eprints-383712018-01-15T06:24:08Z http://eprints.usm.my/38371/ Association of anti-CLIC2 and anti-HMGB1 autoantibodies with higher disease activity in systemic lupus erythematosus patients CS, Syahidatulamali WG, Wan Syamimee Y, Nor Azwany Wong, KK CH, Che Maraina QR180-189.5 Immunology Systemic lupus erythematosus (SLE) is a systemic autoimmune disease characterized by numerous autoantibodies. In this study, we investigated the presence of anti-chloride intracellular channel 2 (anti-CLIC2) and anti-high mobility group box 1 (anti-HMGB1) autoantibodies in SLE patients (n = 43) versus healthy controls ([HCs] n = 43), and their association with serological parameters (antinuclear antibody [ANA], anti-double-stranded DNA [anti-dsDNA], and C-reactive protein [CRP]) and disease activity using Systemic Lupus Erythematosus Disease Activity Index (SLEDAI) score (active or inactive). Settings and Design: Case– control study at Rheumatology Clinic of Universiti Sains Malaysia Hospital. Subjects and Methods: The sera of SLE patients and HCs were tested for the presence of anti-CLIC2 and anti-HMGB1 autoantibodies using human recombinant proteins and ELISA methodologies. Other serological parameters were evaluated according to routine procedures, and patients' demographic and clinical data were obtained. Statistical Analysis: Mann– Whitney U-test, Chi-square test, Fisher's exact test, and receiver operating characteristic analysis. Results: Anti-CLIC2 autoantibody levels were significantly higher in SLE patients compared to HCs (P = 0.0035), whereas anti-HMGB1 autoantibody levels were not significantly elevated (P = 0.7702). Anti-CLIC2 and anti-HMGB1 autoantibody levels were not associated with ANA pattern, anti-dsDNA, and CRP. Interestingly, SLEDAI score (≥6) was associated with anti-CLIC2 (P = 0.0046) and with anti-HMGB1 (P = 0.0091) autoantibody levels. Conclusion: Our findings support the potential of using anti-CLIC2 autoantibodies as a novel biomarker for SLE patients. Both anti-CLIC2 and anti-HMGB1 autoantibody levels demonstrated potential in monitoring SLE disease activity. Medknow Publications 2017 Article PeerReviewed application/pdf en http://eprints.usm.my/38371/1/Association_of_anti-CLIC2_and_anti-HMGB1_autoantibodies.pdf CS, Syahidatulamali and WG, Wan Syamimee and Y, Nor Azwany and Wong, KK and CH, Che Maraina (2017) Association of anti-CLIC2 and anti-HMGB1 autoantibodies with higher disease activity in systemic lupus erythematosus patients. Journal of Postgraduate Medicine, 63 (4). pp. 257-261. ISSN 0022-3859 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5664871/ |
| spellingShingle | QR180-189.5 Immunology CS, Syahidatulamali WG, Wan Syamimee Y, Nor Azwany Wong, KK CH, Che Maraina Association of anti-CLIC2 and anti-HMGB1 autoantibodies with higher disease activity in systemic lupus erythematosus patients |
| title | Association of anti-CLIC2 and anti-HMGB1 autoantibodies with higher disease activity in systemic lupus erythematosus patients |
| title_full | Association of anti-CLIC2 and anti-HMGB1 autoantibodies with higher disease activity in systemic lupus erythematosus patients |
| title_fullStr | Association of anti-CLIC2 and anti-HMGB1 autoantibodies with higher disease activity in systemic lupus erythematosus patients |
| title_full_unstemmed | Association of anti-CLIC2 and anti-HMGB1 autoantibodies with higher disease activity in systemic lupus erythematosus patients |
| title_short | Association of anti-CLIC2 and anti-HMGB1 autoantibodies with higher disease activity in systemic lupus erythematosus patients |
| title_sort | association of anti clic2 and anti hmgb1 autoantibodies with higher disease activity in systemic lupus erythematosus patients |
| topic | QR180-189.5 Immunology |
| url | http://eprints.usm.my/38371/1/Association_of_anti-CLIC2_and_anti-HMGB1_autoantibodies.pdf |
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