Comparative Analysis of Lycorine in Wild Plant and Callus Culture Samples of Hymenocallis littoralis by HPLC-UV Method
The Hymenocallis littoralis, an ornamental and medicinal plant, had been traditionally used for wound healing. In the present study, an analytical method using HPLC with ultraviolet detection was developed for the quantification of lycorine in the extracts of different parts of wild plant and tiss...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Hindawi Publishing Corporation
2014
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| Subjects: | |
| Online Access: | http://eprints.usm.my/38468/1/Comparative_Analysis_of_Lycorine_in_Wild_Plant_and_Callus_Culture_Samples_of_Hymenocallis.pdf |
| Summary: | The Hymenocallis littoralis, an ornamental and medicinal plant, had been traditionally used for wound healing. In the present
study, an analytical method using HPLC with ultraviolet detection was developed for the quantification of lycorine in the extracts
of different parts of wild plant and tissue culture samples of H. littoralis.The separationwas achieved using a reversed-phase column.
The method was found to be accurate, repeatable, and sensitive for the quantification of minute amount of lycorine present in the
samples. The highest lycorine content was found in the bulb extract (2.54 ± 0.02 |
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