Cryopreservation of PLBs of Brassidium Fly Away Using Encapsulation-Dehydration Techniqu
In vitro grown protocorm-like bodies (PLBs) of Brassidium Fly Away orchid hybrid were cryopreserved using encapsulation- dehydration technique. The viability of the cryopreserved cells was determined by 2,3,5-triphenyltetrazolium chloride (TTC) assay. For the preculture treatment, the PLBs were e...
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| Format: | Article |
| Language: | English |
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National University of Mongolia
2015
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| Online Access: | http://eprints.usm.my/38552/1/Cryopreservation_of_PLBs_of_Brassidium_Fly_Away_Using.pdf |
| _version_ | 1797010055354122240 |
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| author | Rajasegar, Arulvilee Poobathy, Ranjetta Rathinam, Xavier Oyunbileg, Yungeree Subramaniam, Sreeramanan |
| author_facet | Rajasegar, Arulvilee Poobathy, Ranjetta Rathinam, Xavier Oyunbileg, Yungeree Subramaniam, Sreeramanan |
| author_sort | Rajasegar, Arulvilee |
| collection | USM |
| description | In vitro grown protocorm-like bodies (PLBs) of Brassidium Fly Away orchid hybrid
were cryopreserved using encapsulation- dehydration technique. The viability of the
cryopreserved cells was determined by 2,3,5-triphenyltetrazolium chloride (TTC)
assay. For the preculture treatment, the PLBs were excised into two standard sizes of
1-2 and 4-5 mm and were precultured on half-strength Murashige and Skoog (MS)
semi solid medium supplemented with diff erent concentrations of sucrose (0, 0.2,
0.4, 0.6, 0.8 and 1.0M). The PLBs size 4-5 mm and 0.6 M sucrose concentration
was selected based on highest viability obtained in TTC assay. The PLBs were
encapsulated for 30 minutes using 3% (w/v) liquid sodium alginate medium
supplemented with 0.4M sucrose and 0.1M calcium chloride and osmoprotected
in 0.75M sucrose solution for 24 hours at 25°C. The beads were then dehydrated
using 50g heat-sterilised silica gel for four hours, cryopreserved for 24 hours,
thawed in a 40±2°C water bath for 90 seconds, and regenerated in semi-solid
half-strength. Biochemical analyses were conducted and the cryopreserved PLBs
had produced lower content of chlorophyll while the highest specifi c peroxidase
activity was observed in cryopreserved PLBs. |
| first_indexed | 2024-03-06T15:14:21Z |
| format | Article |
| id | usm.eprints-38552 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T15:14:21Z |
| publishDate | 2015 |
| publisher | National University of Mongolia |
| record_format | dspace |
| spelling | usm.eprints-385522018-01-25T08:16:36Z http://eprints.usm.my/38552/ Cryopreservation of PLBs of Brassidium Fly Away Using Encapsulation-Dehydration Techniqu Rajasegar, Arulvilee Poobathy, Ranjetta Rathinam, Xavier Oyunbileg, Yungeree Subramaniam, Sreeramanan QH1 Natural history (General - Including nature conservation, geographical distribution) In vitro grown protocorm-like bodies (PLBs) of Brassidium Fly Away orchid hybrid were cryopreserved using encapsulation- dehydration technique. The viability of the cryopreserved cells was determined by 2,3,5-triphenyltetrazolium chloride (TTC) assay. For the preculture treatment, the PLBs were excised into two standard sizes of 1-2 and 4-5 mm and were precultured on half-strength Murashige and Skoog (MS) semi solid medium supplemented with diff erent concentrations of sucrose (0, 0.2, 0.4, 0.6, 0.8 and 1.0M). The PLBs size 4-5 mm and 0.6 M sucrose concentration was selected based on highest viability obtained in TTC assay. The PLBs were encapsulated for 30 minutes using 3% (w/v) liquid sodium alginate medium supplemented with 0.4M sucrose and 0.1M calcium chloride and osmoprotected in 0.75M sucrose solution for 24 hours at 25°C. The beads were then dehydrated using 50g heat-sterilised silica gel for four hours, cryopreserved for 24 hours, thawed in a 40±2°C water bath for 90 seconds, and regenerated in semi-solid half-strength. Biochemical analyses were conducted and the cryopreserved PLBs had produced lower content of chlorophyll while the highest specifi c peroxidase activity was observed in cryopreserved PLBs. National University of Mongolia 2015 Article PeerReviewed application/pdf en http://eprints.usm.my/38552/1/Cryopreservation_of_PLBs_of_Brassidium_Fly_Away_Using.pdf Rajasegar, Arulvilee and Poobathy, Ranjetta and Rathinam, Xavier and Oyunbileg, Yungeree and Subramaniam, Sreeramanan (2015) Cryopreservation of PLBs of Brassidium Fly Away Using Encapsulation-Dehydration Techniqu. Mongolian Journal of Biological Sciences, 13 (1-2). pp. 19-23. ISSN 1684-3908 http://dx.doi.org/10.22353/mjbs.2015.13.03 |
| spellingShingle | QH1 Natural history (General - Including nature conservation, geographical distribution) Rajasegar, Arulvilee Poobathy, Ranjetta Rathinam, Xavier Oyunbileg, Yungeree Subramaniam, Sreeramanan Cryopreservation of PLBs of Brassidium Fly Away Using Encapsulation-Dehydration Techniqu |
| title | Cryopreservation of PLBs of Brassidium Fly Away Using
Encapsulation-Dehydration Techniqu |
| title_full | Cryopreservation of PLBs of Brassidium Fly Away Using
Encapsulation-Dehydration Techniqu |
| title_fullStr | Cryopreservation of PLBs of Brassidium Fly Away Using
Encapsulation-Dehydration Techniqu |
| title_full_unstemmed | Cryopreservation of PLBs of Brassidium Fly Away Using
Encapsulation-Dehydration Techniqu |
| title_short | Cryopreservation of PLBs of Brassidium Fly Away Using
Encapsulation-Dehydration Techniqu |
| title_sort | cryopreservation of plbs of brassidium fly away using encapsulation dehydration techniqu |
| topic | QH1 Natural history (General - Including nature conservation, geographical distribution) |
| url | http://eprints.usm.my/38552/1/Cryopreservation_of_PLBs_of_Brassidium_Fly_Away_Using.pdf |
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