Cucurbitacin L 2-O-β-Glucoside Demonstrates Apoptogenesis in Colon Adenocarcinoma Cells (HT-29): Involvement of Reactive Oxygen and Nitrogen Species Regulation
Emerging evidence suggests that reactive oxygen (ROS) and nitrogen (RNS) species can contribute to diverse signalling pathways of inflammatory and tumour cells. Cucurbitacins are a group of highly oxygenated triterpenes. Many plants used in folk medicine to treat cancer have been found to contain...
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| Format: | Article |
| Language: | English |
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Hindawi Publishing Corporation
2012
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| Online Access: | http://eprints.usm.my/38597/1/Cucurbitacin_L_2-O-%CE%B2-Glucoside_Demonstrates_Apoptogenesis_in_Colon_Adenocarcinoma_Cells.pdf |
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| author | Abdelwahab, Siddig Ibrahim Hassan, Loiy Elsir Ahmed Abdul Majid, Amin M. S. Ahmed Yagi, Sakina M. Mohan, Syam Mohamed Elhassan Taha, Manal Ahmad, Syahida Shiau Chuen, Cheah Mohd Fauzi, Putri Narrima Rais, Mohd Mustafa Syam, Suvitha Abdulkarim Moharam, Bushra A. Hadi, A. Hamid |
| author_facet | Abdelwahab, Siddig Ibrahim Hassan, Loiy Elsir Ahmed Abdul Majid, Amin M. S. Ahmed Yagi, Sakina M. Mohan, Syam Mohamed Elhassan Taha, Manal Ahmad, Syahida Shiau Chuen, Cheah Mohd Fauzi, Putri Narrima Rais, Mohd Mustafa Syam, Suvitha Abdulkarim Moharam, Bushra A. Hadi, A. Hamid |
| author_sort | Abdelwahab, Siddig Ibrahim |
| collection | USM |
| description | Emerging evidence suggests that reactive oxygen (ROS) and nitrogen (RNS) species can contribute to diverse signalling pathways of
inflammatory and tumour cells. Cucurbitacins are a group of highly oxygenated triterpenes. Many plants used in folk medicine to
treat cancer have been found to contain cucurbitacins displaying potentially important anti-inflammatory actions. The current
study was designed to investigate the anti-ROS and -RNS effects of cucurbitacin L 2-O-β-glucoside (CLG) and the role of
these signaling factors in the apoptogenic effects of CLG on human colon cancer cells (HT-29). This natural cucurbitacin was
isolated purely from Citrullus lanatus var. citroides (Cucurbitaceae). The results revealed that CLG was cytotoxic to HT-29. CLG
increased significantly (P < 0.05) RNA and protein levels of caspase-3 in HT-29 cells when verified using a colorimetric assay and
realtime qPCR, respectively. The results showed that lipopolysaccharide/interferon-gamma (LPS/INF-γ) increased nitrous oxide
(NO) production inR AW264.7macrophages, whereas N(G)-nitro-L-argininemethyl ester (L-NAME) and CLG curtailed it. This
compound did not reveal any cytotoxicity on RAW264.7 macrophages and human normal liver cells (WRL-68) when tested using
the MTT assay. Findings of ferric reducing antioxidant power (FRAP) and oxygen radical absorption capacity (ORAC) assays
demonstrate the antioxidant properties of CLG. The apoptogenic property of CLG on HT-29 cells is thus related to inhibition of
reactive nitrogen and oxygen reactive species and the triggering of caspase-3-regulated apoptosis. |
| first_indexed | 2024-03-06T15:14:29Z |
| format | Article |
| id | usm.eprints-38597 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T15:14:29Z |
| publishDate | 2012 |
| publisher | Hindawi Publishing Corporation |
| record_format | dspace |
| spelling | usm.eprints-385972018-01-30T00:24:48Z http://eprints.usm.my/38597/ Cucurbitacin L 2-O-β-Glucoside Demonstrates Apoptogenesis in Colon Adenocarcinoma Cells (HT-29): Involvement of Reactive Oxygen and Nitrogen Species Regulation Abdelwahab, Siddig Ibrahim Hassan, Loiy Elsir Ahmed Abdul Majid, Amin M. S. Ahmed Yagi, Sakina M. Mohan, Syam Mohamed Elhassan Taha, Manal Ahmad, Syahida Shiau Chuen, Cheah Mohd Fauzi, Putri Narrima Rais, Mohd Mustafa Syam, Suvitha Abdulkarim Moharam, Bushra A. Hadi, A. Hamid RM Therapeutics. Pharmacology RS1-441 Pharmacy and materia medica Emerging evidence suggests that reactive oxygen (ROS) and nitrogen (RNS) species can contribute to diverse signalling pathways of inflammatory and tumour cells. Cucurbitacins are a group of highly oxygenated triterpenes. Many plants used in folk medicine to treat cancer have been found to contain cucurbitacins displaying potentially important anti-inflammatory actions. The current study was designed to investigate the anti-ROS and -RNS effects of cucurbitacin L 2-O-β-glucoside (CLG) and the role of these signaling factors in the apoptogenic effects of CLG on human colon cancer cells (HT-29). This natural cucurbitacin was isolated purely from Citrullus lanatus var. citroides (Cucurbitaceae). The results revealed that CLG was cytotoxic to HT-29. CLG increased significantly (P < 0.05) RNA and protein levels of caspase-3 in HT-29 cells when verified using a colorimetric assay and realtime qPCR, respectively. The results showed that lipopolysaccharide/interferon-gamma (LPS/INF-γ) increased nitrous oxide (NO) production inR AW264.7macrophages, whereas N(G)-nitro-L-argininemethyl ester (L-NAME) and CLG curtailed it. This compound did not reveal any cytotoxicity on RAW264.7 macrophages and human normal liver cells (WRL-68) when tested using the MTT assay. Findings of ferric reducing antioxidant power (FRAP) and oxygen radical absorption capacity (ORAC) assays demonstrate the antioxidant properties of CLG. The apoptogenic property of CLG on HT-29 cells is thus related to inhibition of reactive nitrogen and oxygen reactive species and the triggering of caspase-3-regulated apoptosis. Hindawi Publishing Corporation 2012 Article PeerReviewed application/pdf en http://eprints.usm.my/38597/1/Cucurbitacin_L_2-O-%CE%B2-Glucoside_Demonstrates_Apoptogenesis_in_Colon_Adenocarcinoma_Cells.pdf Abdelwahab, Siddig Ibrahim and Hassan, Loiy Elsir Ahmed and Abdul Majid, Amin M. S. and Ahmed Yagi, Sakina M. and Mohan, Syam and Mohamed Elhassan Taha, Manal and Ahmad, Syahida and Shiau Chuen, Cheah and Mohd Fauzi, Putri Narrima and Rais, Mohd Mustafa and Syam, Suvitha and Abdulkarim Moharam, Bushra and A. Hadi, A. Hamid (2012) Cucurbitacin L 2-O-β-Glucoside Demonstrates Apoptogenesis in Colon Adenocarcinoma Cells (HT-29): Involvement of Reactive Oxygen and Nitrogen Species Regulation. Evidence-Based Complementary and Alternative Medicine, 2012 (490136). pp. 1-8. ISSN 1741-427X https://doi.org/10.1155/2012/490136 |
| spellingShingle | RM Therapeutics. Pharmacology RS1-441 Pharmacy and materia medica Abdelwahab, Siddig Ibrahim Hassan, Loiy Elsir Ahmed Abdul Majid, Amin M. S. Ahmed Yagi, Sakina M. Mohan, Syam Mohamed Elhassan Taha, Manal Ahmad, Syahida Shiau Chuen, Cheah Mohd Fauzi, Putri Narrima Rais, Mohd Mustafa Syam, Suvitha Abdulkarim Moharam, Bushra A. Hadi, A. Hamid Cucurbitacin L 2-O-β-Glucoside Demonstrates Apoptogenesis in Colon Adenocarcinoma Cells (HT-29): Involvement of Reactive Oxygen and Nitrogen Species Regulation |
| title | Cucurbitacin L 2-O-β-Glucoside Demonstrates Apoptogenesis
in Colon Adenocarcinoma Cells (HT-29): Involvement of Reactive Oxygen and Nitrogen Species Regulation |
| title_full | Cucurbitacin L 2-O-β-Glucoside Demonstrates Apoptogenesis
in Colon Adenocarcinoma Cells (HT-29): Involvement of Reactive Oxygen and Nitrogen Species Regulation |
| title_fullStr | Cucurbitacin L 2-O-β-Glucoside Demonstrates Apoptogenesis
in Colon Adenocarcinoma Cells (HT-29): Involvement of Reactive Oxygen and Nitrogen Species Regulation |
| title_full_unstemmed | Cucurbitacin L 2-O-β-Glucoside Demonstrates Apoptogenesis
in Colon Adenocarcinoma Cells (HT-29): Involvement of Reactive Oxygen and Nitrogen Species Regulation |
| title_short | Cucurbitacin L 2-O-β-Glucoside Demonstrates Apoptogenesis
in Colon Adenocarcinoma Cells (HT-29): Involvement of Reactive Oxygen and Nitrogen Species Regulation |
| title_sort | cucurbitacin l 2 o β glucoside demonstrates apoptogenesis in colon adenocarcinoma cells ht 29 involvement of reactive oxygen and nitrogen species regulation |
| topic | RM Therapeutics. Pharmacology RS1-441 Pharmacy and materia medica |
| url | http://eprints.usm.my/38597/1/Cucurbitacin_L_2-O-%CE%B2-Glucoside_Demonstrates_Apoptogenesis_in_Colon_Adenocarcinoma_Cells.pdf |
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