Development of an Antigen-DNAzyme Based Probe for a Direct Antibody-Antigen Assay Using the Intrinsic DNAzyme Activity of a Daunomycin Aptamer
G-Quadruplex (G-4) structures are formed when G-rich DNA sequences fold into intra- or intermolecular four-stranded structures in the presence of metal ions. G-4-hemin complexes are often effective peroxidase-mimicking DNAzymes that are applied in many detection systems. This work reports the applic...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI
2014
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| Subjects: | |
| Online Access: | http://eprints.usm.my/38675/1/Development_of_an_Antigen-DNAzyme_Based_Probe_for_a_Direct_Antibody-Antigen.pdf |
| _version_ | 1797010080795721728 |
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| author | Omar, Noorsharmimi Loh, Qiuting Gee, Jun Tye Yee, Siew Choong Noordin, Rahmah Jörn, Glökler Lim, Theam Soon |
| author_facet | Omar, Noorsharmimi Loh, Qiuting Gee, Jun Tye Yee, Siew Choong Noordin, Rahmah Jörn, Glökler Lim, Theam Soon |
| author_sort | Omar, Noorsharmimi |
| collection | USM |
| description | G-Quadruplex (G-4) structures are formed when G-rich DNA sequences fold into intra- or intermolecular four-stranded structures in the presence of metal ions. G-4-hemin complexes are often effective peroxidase-mimicking DNAzymes that are applied in many detection systems. This work reports the application of a G-rich daunomycin-specific aptamer for the development of an antibody-antigen detection assay. We investigated the ability of the daunomycin aptamer to efficiently catalyze the hemin-dependent peroxidase activity independent of daunomycin. A reporter probe consisting of biotinylated antigen and daunomycin aptamer coupled to streptavidin gold nanoparticles was successfully used to generate a colorimetric readout. In conclusion, the daunomycin aptamer can function as a robust alternative DNAzyme for the development of colorimetric assays. |
| first_indexed | 2024-03-06T15:14:42Z |
| format | Article |
| id | usm.eprints-38675 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T15:14:42Z |
| publishDate | 2014 |
| publisher | MDPI |
| record_format | dspace |
| spelling | usm.eprints-386752018-02-02T09:20:39Z http://eprints.usm.my/38675/ Development of an Antigen-DNAzyme Based Probe for a Direct Antibody-Antigen Assay Using the Intrinsic DNAzyme Activity of a Daunomycin Aptamer Omar, Noorsharmimi Loh, Qiuting Gee, Jun Tye Yee, Siew Choong Noordin, Rahmah Jörn, Glökler Lim, Theam Soon R735-854 Medical education. Medical schools. Research G-Quadruplex (G-4) structures are formed when G-rich DNA sequences fold into intra- or intermolecular four-stranded structures in the presence of metal ions. G-4-hemin complexes are often effective peroxidase-mimicking DNAzymes that are applied in many detection systems. This work reports the application of a G-rich daunomycin-specific aptamer for the development of an antibody-antigen detection assay. We investigated the ability of the daunomycin aptamer to efficiently catalyze the hemin-dependent peroxidase activity independent of daunomycin. A reporter probe consisting of biotinylated antigen and daunomycin aptamer coupled to streptavidin gold nanoparticles was successfully used to generate a colorimetric readout. In conclusion, the daunomycin aptamer can function as a robust alternative DNAzyme for the development of colorimetric assays. MDPI 2014 Article PeerReviewed application/pdf en http://eprints.usm.my/38675/1/Development_of_an_Antigen-DNAzyme_Based_Probe_for_a_Direct_Antibody-Antigen.pdf Omar, Noorsharmimi and Loh, Qiuting and Gee, Jun Tye and Yee, Siew Choong and Noordin, Rahmah and Jörn, Glökler and Lim, Theam Soon (2014) Development of an Antigen-DNAzyme Based Probe for a Direct Antibody-Antigen Assay Using the Intrinsic DNAzyme Activity of a Daunomycin Aptamer. Sensors, 14 (1). pp. 346-355. ISSN 1424-8220 https://doi.org/10.3390/s140100346 |
| spellingShingle | R735-854 Medical education. Medical schools. Research Omar, Noorsharmimi Loh, Qiuting Gee, Jun Tye Yee, Siew Choong Noordin, Rahmah Jörn, Glökler Lim, Theam Soon Development of an Antigen-DNAzyme Based Probe for a Direct Antibody-Antigen Assay Using the Intrinsic DNAzyme Activity of a Daunomycin Aptamer |
| title | Development of an Antigen-DNAzyme Based Probe for a Direct Antibody-Antigen Assay Using the Intrinsic DNAzyme Activity of a Daunomycin Aptamer |
| title_full | Development of an Antigen-DNAzyme Based Probe for a Direct Antibody-Antigen Assay Using the Intrinsic DNAzyme Activity of a Daunomycin Aptamer |
| title_fullStr | Development of an Antigen-DNAzyme Based Probe for a Direct Antibody-Antigen Assay Using the Intrinsic DNAzyme Activity of a Daunomycin Aptamer |
| title_full_unstemmed | Development of an Antigen-DNAzyme Based Probe for a Direct Antibody-Antigen Assay Using the Intrinsic DNAzyme Activity of a Daunomycin Aptamer |
| title_short | Development of an Antigen-DNAzyme Based Probe for a Direct Antibody-Antigen Assay Using the Intrinsic DNAzyme Activity of a Daunomycin Aptamer |
| title_sort | development of an antigen dnazyme based probe for a direct antibody antigen assay using the intrinsic dnazyme activity of a daunomycin aptamer |
| topic | R735-854 Medical education. Medical schools. Research |
| url | http://eprints.usm.my/38675/1/Development_of_an_Antigen-DNAzyme_Based_Probe_for_a_Direct_Antibody-Antigen.pdf |
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