Evaluation of hepatoprotective effect of methanolic extract of Clitoria ternatea (Linn.) flower against acetaminophen-induced liver damage
Objective To evaluate the hepatoprotective and antioxidant activity of Clitoria ternatea (C. ternatea) flower extract against acetaminophen-induced liver toxicity. Methods The antioxidant property of C. ternatea flower extract was investigated by employing established in vitro antioxidant assay...
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| Format: | Article |
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Elsevier
2013
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| _version_ | 1825833968909418496 |
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| author | Nithianantham, Kuppan Kwan, Yuet Ping Yoga Latha, Lachimanan L. Jothy, Subramanion Ibrahim, Darah Yeng, Chen Chew, Ai Lan Sasidharan, Sreenivasan |
| author_facet | Nithianantham, Kuppan Kwan, Yuet Ping Yoga Latha, Lachimanan L. Jothy, Subramanion Ibrahim, Darah Yeng, Chen Chew, Ai Lan Sasidharan, Sreenivasan |
| author_sort | Nithianantham, Kuppan |
| collection | USM |
| description | Objective
To evaluate the hepatoprotective and antioxidant activity of Clitoria ternatea (C. ternatea) flower extract against acetaminophen-induced liver toxicity.
Methods
The antioxidant property of C. ternatea flower extract was investigated by employing established in vitro antioxidant assay. The C. ternatea flower extract was studied in this work for its hepatoprotective effect against acetaminophen-induced liver toxicity in mice. Activity was measured by monitoring the levels of aspartate aminotransferase, alanine aminotransferase, billirubin and glutathione with histopathological analysis.
Results
The amount of total phenolics and flavonoids were estimated to be 105.40±2.47 mg/g gallic acid equivalent and 72.21±0.05 mg/g catechin equivalent respectively. The antioxidant activity of C. ternatea flower extract was 68.9% at a concentration of 1 mg/mL and was also concentration dependant, with an IC50 value of 327.00 μg/mL. The results of acetaminophen-induced liver toxicity experiment showed that mice treated with the extract (200 mg/kg) showed a significant decrease in alanine aminotransferase, aspartate aminotransferase, and bilirubin levels, which were all elevated in the paracetamol group (P<0.05). Meanwhile, the level of glutathione was found to be restored in extract treated animals compared to the groups treated with acetaminophen alone (P<0.05). Therapy of extract also showed its protective effect on histopathological alterations and supported the biochemical finding. |
| first_indexed | 2024-03-06T15:15:42Z |
| format | Article |
| id | usm.eprints-39013 |
| institution | Universiti Sains Malaysia |
| last_indexed | 2024-03-06T15:15:42Z |
| publishDate | 2013 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | usm.eprints-390132018-02-19T09:18:10Z http://eprints.usm.my/39013/ Evaluation of hepatoprotective effect of methanolic extract of Clitoria ternatea (Linn.) flower against acetaminophen-induced liver damage Nithianantham, Kuppan Kwan, Yuet Ping Yoga Latha, Lachimanan L. Jothy, Subramanion Ibrahim, Darah Yeng, Chen Chew, Ai Lan Sasidharan, Sreenivasan QH1 Natural history (General - Including nature conservation, geographical distribution) R735-854 Medical education. Medical schools. Research Objective To evaluate the hepatoprotective and antioxidant activity of Clitoria ternatea (C. ternatea) flower extract against acetaminophen-induced liver toxicity. Methods The antioxidant property of C. ternatea flower extract was investigated by employing established in vitro antioxidant assay. The C. ternatea flower extract was studied in this work for its hepatoprotective effect against acetaminophen-induced liver toxicity in mice. Activity was measured by monitoring the levels of aspartate aminotransferase, alanine aminotransferase, billirubin and glutathione with histopathological analysis. Results The amount of total phenolics and flavonoids were estimated to be 105.40±2.47 mg/g gallic acid equivalent and 72.21±0.05 mg/g catechin equivalent respectively. The antioxidant activity of C. ternatea flower extract was 68.9% at a concentration of 1 mg/mL and was also concentration dependant, with an IC50 value of 327.00 μg/mL. The results of acetaminophen-induced liver toxicity experiment showed that mice treated with the extract (200 mg/kg) showed a significant decrease in alanine aminotransferase, aspartate aminotransferase, and bilirubin levels, which were all elevated in the paracetamol group (P<0.05). Meanwhile, the level of glutathione was found to be restored in extract treated animals compared to the groups treated with acetaminophen alone (P<0.05). Therapy of extract also showed its protective effect on histopathological alterations and supported the biochemical finding. Elsevier 2013-12 Article PeerReviewed Nithianantham, Kuppan and Kwan, Yuet Ping and Yoga Latha, Lachimanan and L. Jothy, Subramanion and Ibrahim, Darah and Yeng, Chen and Chew, Ai Lan and Sasidharan, Sreenivasan (2013) Evaluation of hepatoprotective effect of methanolic extract of Clitoria ternatea (Linn.) flower against acetaminophen-induced liver damage. Asian Pacific Journal of Tropical Disease, 3 (4). pp. 314-319. ISSN 2222-1808 https://doi.org/10.1016/S2222-1808(13)60075-4 |
| spellingShingle | QH1 Natural history (General - Including nature conservation, geographical distribution) R735-854 Medical education. Medical schools. Research Nithianantham, Kuppan Kwan, Yuet Ping Yoga Latha, Lachimanan L. Jothy, Subramanion Ibrahim, Darah Yeng, Chen Chew, Ai Lan Sasidharan, Sreenivasan Evaluation of hepatoprotective effect of methanolic extract of Clitoria ternatea (Linn.) flower against acetaminophen-induced liver damage |
| title | Evaluation of hepatoprotective effect of methanolic extract of Clitoria ternatea (Linn.) flower against acetaminophen-induced liver damage |
| title_full | Evaluation of hepatoprotective effect of methanolic extract of Clitoria ternatea (Linn.) flower against acetaminophen-induced liver damage |
| title_fullStr | Evaluation of hepatoprotective effect of methanolic extract of Clitoria ternatea (Linn.) flower against acetaminophen-induced liver damage |
| title_full_unstemmed | Evaluation of hepatoprotective effect of methanolic extract of Clitoria ternatea (Linn.) flower against acetaminophen-induced liver damage |
| title_short | Evaluation of hepatoprotective effect of methanolic extract of Clitoria ternatea (Linn.) flower against acetaminophen-induced liver damage |
| title_sort | evaluation of hepatoprotective effect of methanolic extract of clitoria ternatea linn flower against acetaminophen induced liver damage |
| topic | QH1 Natural history (General - Including nature conservation, geographical distribution) R735-854 Medical education. Medical schools. Research |
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