Evaluation of the Genotoxic Potential against H2O2-Radical-Mediated DNA Damage and Acute Oral Toxicity of Standardized Extract of Polyalthia longifolia Leaf
Medicinal plants have been used in medicoculturally diverse countries around the world, where it is a part of a time-honoured tradition that is respected even today. Polyalthia longifolia leaf extract has been previously reported as an efficient antioxidant in vitro.Hence, the genotoxic effects of...
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| Format: | Article |
| Language: | English |
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Hindawi Publishing Corporation
2013
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| Online Access: | http://eprints.usm.my/39015/1/Evaluation_of_the_Genotoxic_Potential_against_H2O2_-Radical-Mediated_DNA_Damage.pdf |
| _version_ | 1797010154969890816 |
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| author | L. Jothy, Subramanion Yeng, Chen Kanwar, Jagat R Sasidharan, Sreenivasan |
| author_facet | L. Jothy, Subramanion Yeng, Chen Kanwar, Jagat R Sasidharan, Sreenivasan |
| author_sort | L. Jothy, Subramanion |
| collection | USM |
| description | Medicinal plants have been used in medicoculturally diverse countries around the world, where it is a part of a time-honoured
tradition that is respected even today. Polyalthia longifolia leaf extract has been previously reported as an efficient antioxidant in
vitro.Hence, the genotoxic effects of P. longifolia leafwere investigated by using plasmid relation, comet, andAllium cepa assay. In the
presence of ∙OHradicals, theDNAin supercoilwas start nicked into open circular form,which is the product of the single-stranded
cleavage of supercoil DNA and quantified as fragmented separate bands on agarose gel in plasmid relation assay. In the plasmid
relation and comet assay, the P. longifolia leaf extract exhibited strong inhibitory effects against H2O2-mediated DNA damage. A
dose-dependent increase of chromosome aberrations was also observed in the Allium cepa assay. The abnormalities scored were
stickiness, c-mitosis, bridges, and vagrant chromosomes. Micronucleated cells were also observed at the interphase. The results of
Allium cepa assay confirmed that the methanol extracts of P. longifolia exerted no significant genotoxic or mitodepressive effects at
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| first_indexed | 2024-03-06T15:15:42Z |
| format | Article |
| id | usm.eprints-39015 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T15:15:42Z |
| publishDate | 2013 |
| publisher | Hindawi Publishing Corporation |
| record_format | dspace |
| spelling | usm.eprints-390152018-02-20T01:08:33Z http://eprints.usm.my/39015/ Evaluation of the Genotoxic Potential against H2O2-Radical-Mediated DNA Damage and Acute Oral Toxicity of Standardized Extract of Polyalthia longifolia Leaf L. Jothy, Subramanion Yeng, Chen Kanwar, Jagat R Sasidharan, Sreenivasan R735-854 Medical education. Medical schools. Research Medicinal plants have been used in medicoculturally diverse countries around the world, where it is a part of a time-honoured tradition that is respected even today. Polyalthia longifolia leaf extract has been previously reported as an efficient antioxidant in vitro.Hence, the genotoxic effects of P. longifolia leafwere investigated by using plasmid relation, comet, andAllium cepa assay. In the presence of ∙OHradicals, theDNAin supercoilwas start nicked into open circular form,which is the product of the single-stranded cleavage of supercoil DNA and quantified as fragmented separate bands on agarose gel in plasmid relation assay. In the plasmid relation and comet assay, the P. longifolia leaf extract exhibited strong inhibitory effects against H2O2-mediated DNA damage. A dose-dependent increase of chromosome aberrations was also observed in the Allium cepa assay. The abnormalities scored were stickiness, c-mitosis, bridges, and vagrant chromosomes. Micronucleated cells were also observed at the interphase. The results of Allium cepa assay confirmed that the methanol extracts of P. longifolia exerted no significant genotoxic or mitodepressive effects at 100 Hindawi Publishing Corporation 2013 Article PeerReviewed application/pdf en http://eprints.usm.my/39015/1/Evaluation_of_the_Genotoxic_Potential_against_H2O2_-Radical-Mediated_DNA_Damage.pdf L. Jothy, Subramanion and Yeng, Chen and Kanwar, Jagat R and Sasidharan, Sreenivasan (2013) Evaluation of the Genotoxic Potential against H2O2-Radical-Mediated DNA Damage and Acute Oral Toxicity of Standardized Extract of Polyalthia longifolia Leaf. Evidence-Based Complementary and Alternative Medicine, 2013 (925380). pp. 1-13. ISSN 1741-427X http://dx.doi.org/10.1155/2013/925380 |
| spellingShingle | R735-854 Medical education. Medical schools. Research L. Jothy, Subramanion Yeng, Chen Kanwar, Jagat R Sasidharan, Sreenivasan Evaluation of the Genotoxic Potential against H2O2-Radical-Mediated DNA Damage and Acute Oral Toxicity of Standardized Extract of Polyalthia longifolia Leaf |
| title | Evaluation of the Genotoxic Potential against H2O2-Radical-Mediated DNA Damage and Acute Oral Toxicity of Standardized Extract of Polyalthia longifolia Leaf |
| title_full | Evaluation of the Genotoxic Potential against H2O2-Radical-Mediated DNA Damage and Acute Oral Toxicity of Standardized Extract of Polyalthia longifolia Leaf |
| title_fullStr | Evaluation of the Genotoxic Potential against H2O2-Radical-Mediated DNA Damage and Acute Oral Toxicity of Standardized Extract of Polyalthia longifolia Leaf |
| title_full_unstemmed | Evaluation of the Genotoxic Potential against H2O2-Radical-Mediated DNA Damage and Acute Oral Toxicity of Standardized Extract of Polyalthia longifolia Leaf |
| title_short | Evaluation of the Genotoxic Potential against H2O2-Radical-Mediated DNA Damage and Acute Oral Toxicity of Standardized Extract of Polyalthia longifolia Leaf |
| title_sort | evaluation of the genotoxic potential against h2o2 radical mediated dna damage and acute oral toxicity of standardized extract of polyalthia longifolia leaf |
| topic | R735-854 Medical education. Medical schools. Research |
| url | http://eprints.usm.my/39015/1/Evaluation_of_the_Genotoxic_Potential_against_H2O2_-Radical-Mediated_DNA_Damage.pdf |
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