Evaluation of α-Glucosidase Inhibitory Effect of 50% Ethanolic Standardized Extract of Orthosiphon stamineus Benth in Normal and Streptozotocin-Induced Diabetic Rats
In the present study, a 50% ethanolic extract of Orthosiphon stamineus was tested for its α-glucosidase inhibitory activity. In vivo assays of the extract (containing 1.02%, 3.76%, and 3.03% of 3′hydroxy-5,6,7,4′-tetramethoxyflavone, sinensetin, and eupatorin, resp.) showed that it possessed an inhi...
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| Format: | Article |
| Language: | English |
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Hindawi Publishing Corporation
2015
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| Online Access: | http://eprints.usm.my/39039/1/Evaluation_of_%CE%B1-Glucosidase_Inhibitory_Effect_of_50%25_Ethanolic_Standardized.pdf |
| _version_ | 1825833973636399104 |
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| author | Mohamed, Elsnoussi Ali Ahmad, Mariam Lee, Fung Ang Asmawi, Mohd. Zaini Mun, Fei Yam |
| author_facet | Mohamed, Elsnoussi Ali Ahmad, Mariam Lee, Fung Ang Asmawi, Mohd. Zaini Mun, Fei Yam |
| author_sort | Mohamed, Elsnoussi Ali |
| collection | USM |
| description | In the present study, a 50% ethanolic extract of Orthosiphon stamineus was tested for its α-glucosidase inhibitory activity. In vivo assays of the extract (containing 1.02%, 3.76%, and 3.03% of 3′hydroxy-5,6,7,4′-tetramethoxyflavone, sinensetin, and eupatorin, resp.) showed that it possessed an inhibitory activity against α-glucosidase in normal rats loaded with starch and sucrose. The results showed that 1000 mg/kg of the 50% ethanolic extract of O. stamineus significantly () decreased the plasma glucose levels of the experimental animals in a manner resembling the effect of acarbose. In streptozotocin-induced diabetic rats, only the group treated with 1000 mg/kg of the extract showed significantly () lower plasma glucose levels after starch loading. Hence, α-glucosidase inhibition might be one of the mechanisms by which O. stamineus extract exerts its antidiabetic effect. Furthermore, our findings indicated that the 50% ethanolic extract of O. stamineus can be considered as a potential agent for the management of diabetes mellitus. |
| first_indexed | 2024-03-06T15:15:46Z |
| format | Article |
| id | usm.eprints-39039 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T15:15:46Z |
| publishDate | 2015 |
| publisher | Hindawi Publishing Corporation |
| record_format | dspace |
| spelling | usm.eprints-390392018-02-20T02:30:45Z http://eprints.usm.my/39039/ Evaluation of α-Glucosidase Inhibitory Effect of 50% Ethanolic Standardized Extract of Orthosiphon stamineus Benth in Normal and Streptozotocin-Induced Diabetic Rats Mohamed, Elsnoussi Ali Ahmad, Mariam Lee, Fung Ang Asmawi, Mohd. Zaini Mun, Fei Yam RS1-441 Pharmacy and materia medica In the present study, a 50% ethanolic extract of Orthosiphon stamineus was tested for its α-glucosidase inhibitory activity. In vivo assays of the extract (containing 1.02%, 3.76%, and 3.03% of 3′hydroxy-5,6,7,4′-tetramethoxyflavone, sinensetin, and eupatorin, resp.) showed that it possessed an inhibitory activity against α-glucosidase in normal rats loaded with starch and sucrose. The results showed that 1000 mg/kg of the 50% ethanolic extract of O. stamineus significantly () decreased the plasma glucose levels of the experimental animals in a manner resembling the effect of acarbose. In streptozotocin-induced diabetic rats, only the group treated with 1000 mg/kg of the extract showed significantly () lower plasma glucose levels after starch loading. Hence, α-glucosidase inhibition might be one of the mechanisms by which O. stamineus extract exerts its antidiabetic effect. Furthermore, our findings indicated that the 50% ethanolic extract of O. stamineus can be considered as a potential agent for the management of diabetes mellitus. Hindawi Publishing Corporation 2015 Article PeerReviewed application/pdf en http://eprints.usm.my/39039/1/Evaluation_of_%CE%B1-Glucosidase_Inhibitory_Effect_of_50%25_Ethanolic_Standardized.pdf Mohamed, Elsnoussi Ali and Ahmad, Mariam and Lee, Fung Ang and Asmawi, Mohd. Zaini and Mun, Fei Yam (2015) Evaluation of α-Glucosidase Inhibitory Effect of 50% Ethanolic Standardized Extract of Orthosiphon stamineus Benth in Normal and Streptozotocin-Induced Diabetic Rats. Evidence-Based Complementary and Alternative Medicine, 2015 (754931). pp. 1-6. ISSN 1741-427X http://dx.doi.org/10.1155/2015/754931 |
| spellingShingle | RS1-441 Pharmacy and materia medica Mohamed, Elsnoussi Ali Ahmad, Mariam Lee, Fung Ang Asmawi, Mohd. Zaini Mun, Fei Yam Evaluation of α-Glucosidase Inhibitory Effect of 50% Ethanolic Standardized Extract of Orthosiphon stamineus Benth in Normal and Streptozotocin-Induced Diabetic Rats |
| title | Evaluation of α-Glucosidase Inhibitory Effect of 50% Ethanolic Standardized Extract of Orthosiphon stamineus Benth in Normal and Streptozotocin-Induced Diabetic Rats |
| title_full | Evaluation of α-Glucosidase Inhibitory Effect of 50% Ethanolic Standardized Extract of Orthosiphon stamineus Benth in Normal and Streptozotocin-Induced Diabetic Rats |
| title_fullStr | Evaluation of α-Glucosidase Inhibitory Effect of 50% Ethanolic Standardized Extract of Orthosiphon stamineus Benth in Normal and Streptozotocin-Induced Diabetic Rats |
| title_full_unstemmed | Evaluation of α-Glucosidase Inhibitory Effect of 50% Ethanolic Standardized Extract of Orthosiphon stamineus Benth in Normal and Streptozotocin-Induced Diabetic Rats |
| title_short | Evaluation of α-Glucosidase Inhibitory Effect of 50% Ethanolic Standardized Extract of Orthosiphon stamineus Benth in Normal and Streptozotocin-Induced Diabetic Rats |
| title_sort | evaluation of α glucosidase inhibitory effect of 50 ethanolic standardized extract of orthosiphon stamineus benth in normal and streptozotocin induced diabetic rats |
| topic | RS1-441 Pharmacy and materia medica |
| url | http://eprints.usm.my/39039/1/Evaluation_of_%CE%B1-Glucosidase_Inhibitory_Effect_of_50%25_Ethanolic_Standardized.pdf |
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