Development of Multiplexed Infectious Disease Lateral Flow Assays: Challenges and Opportunities
Lateral flow assays (LFAs) are the mainstay of rapid point-of-care diagnostics, with the potential to enable early case management and transform the epidemiology of infectious disease. However, most LFAs only detect single biomarkers. Recognizing the complex nature of human disease, overlapping symp...
| 主要な著者: | , , , , , |
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| フォーマット: | 論文 |
| 言語: | English |
| 出版事項: |
MDPI
2017
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| 主題: | |
| オンライン･アクセス: | http://eprints.usm.my/40878/1/Development_of_Multiplexed_Infectious_Disease_Late.pdf |
| _version_ | 1825834324795064320 |
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| author | Hanafiah, Khayriyyah Mohd Arifin, Norsyahida Bustami, Yazmin Noordin, Rahmah Garcia, Mary Anderson, David |
| author_facet | Hanafiah, Khayriyyah Mohd Arifin, Norsyahida Bustami, Yazmin Noordin, Rahmah Garcia, Mary Anderson, David |
| author_sort | Hanafiah, Khayriyyah Mohd |
| collection | USM |
| description | Lateral flow assays (LFAs) are the mainstay of rapid point-of-care diagnostics, with the potential to enable early case management and transform the epidemiology of infectious disease. However, most LFAs only detect single biomarkers. Recognizing the complex nature of human disease, overlapping symptoms and states of co-infections, there is increasing demand for multiplexed systems that can detect multiple biomarkers simultaneously. Due to innate limitations in the design of traditional membrane-based LFAs, multiplexing is arguably limited to a small number of biomarkers. Here, we summarize the need for multiplexed LFA, key technical and operational challenges for multiplexing, inherent in the design and production of multiplexed LFAs, as well as emerging enabling technologies that may be able to address these challenges. We further identify important areas for research in efforts towards developing multiplexed LFAs for more impactful diagnosis of infectious diseases. |
| first_indexed | 2024-03-06T15:21:02Z |
| format | Article |
| id | usm.eprints-40878 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T15:21:02Z |
| publishDate | 2017 |
| publisher | MDPI |
| record_format | dspace |
| spelling | usm.eprints-408782018-07-03T05:19:45Z http://eprints.usm.my/40878/ Development of Multiplexed Infectious Disease Lateral Flow Assays: Challenges and Opportunities Hanafiah, Khayriyyah Mohd Arifin, Norsyahida Bustami, Yazmin Noordin, Rahmah Garcia, Mary Anderson, David QH301 Biology Lateral flow assays (LFAs) are the mainstay of rapid point-of-care diagnostics, with the potential to enable early case management and transform the epidemiology of infectious disease. However, most LFAs only detect single biomarkers. Recognizing the complex nature of human disease, overlapping symptoms and states of co-infections, there is increasing demand for multiplexed systems that can detect multiple biomarkers simultaneously. Due to innate limitations in the design of traditional membrane-based LFAs, multiplexing is arguably limited to a small number of biomarkers. Here, we summarize the need for multiplexed LFA, key technical and operational challenges for multiplexing, inherent in the design and production of multiplexed LFAs, as well as emerging enabling technologies that may be able to address these challenges. We further identify important areas for research in efforts towards developing multiplexed LFAs for more impactful diagnosis of infectious diseases. MDPI 2017 Article PeerReviewed application/pdf en http://eprints.usm.my/40878/1/Development_of_Multiplexed_Infectious_Disease_Late.pdf Hanafiah, Khayriyyah Mohd and Arifin, Norsyahida and Bustami, Yazmin and Noordin, Rahmah and Garcia, Mary and Anderson, David (2017) Development of Multiplexed Infectious Disease Lateral Flow Assays: Challenges and Opportunities. Diagnostics, 7 (3). p. 51. ISSN 2075-4418 http://dx.doi.org/10.3390/diagnostics7030051 |
| spellingShingle | QH301 Biology Hanafiah, Khayriyyah Mohd Arifin, Norsyahida Bustami, Yazmin Noordin, Rahmah Garcia, Mary Anderson, David Development of Multiplexed Infectious Disease Lateral Flow Assays: Challenges and Opportunities |
| title | Development of Multiplexed Infectious Disease Lateral Flow Assays: Challenges and Opportunities |
| title_full | Development of Multiplexed Infectious Disease Lateral Flow Assays: Challenges and Opportunities |
| title_fullStr | Development of Multiplexed Infectious Disease Lateral Flow Assays: Challenges and Opportunities |
| title_full_unstemmed | Development of Multiplexed Infectious Disease Lateral Flow Assays: Challenges and Opportunities |
| title_short | Development of Multiplexed Infectious Disease Lateral Flow Assays: Challenges and Opportunities |
| title_sort | development of multiplexed infectious disease lateral flow assays challenges and opportunities |
| topic | QH301 Biology |
| url | http://eprints.usm.my/40878/1/Development_of_Multiplexed_Infectious_Disease_Late.pdf |
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