Production And Characterization Of Poly(3-Hydroxybutyrate-Co-4-Hydroxybutyrate) Copolymer
A locally isolated Cupriavidus sp. USMAA2-4 possessing the ability to produce biodegradable polymer was used in this study to explore the production of biopolymer through one-stage cultivation process and fed-batch fermentation. Various palm oilbased carbon sources, fatty acids and its combinatio...
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| Format: | Thesis |
| Language: | English |
| Published: |
2011
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| Subjects: | |
| Online Access: | http://eprints.usm.my/40936/1/Rahayu_bt_Ahmad_24MS_HJ.pdf |
| _version_ | 1797010535590395904 |
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| author | Ahmad, Rahayu |
| author_facet | Ahmad, Rahayu |
| author_sort | Ahmad, Rahayu |
| collection | USM |
| description | A locally isolated Cupriavidus sp. USMAA2-4 possessing the ability to produce
biodegradable polymer was used in this study to explore the production of biopolymer
through one-stage cultivation process and fed-batch fermentation. Various palm oilbased
carbon sources, fatty acids and its combination were used for producing high cell
dry weight of Cupriavidus sp. USMAA2-4. Utilization of oleic acid solely yielding the
highest cell dry weight with 8.4 g/L. Effects of carbon to nitrogen (C/N ratio) on the
accumulation of P(3HB) before feeding of carbon precursor (γ-butyrolactone) exhibited
significant increased of 4HB compositions from 9 to 55 mol% when C/N ratio decreased
from 20-8. The monomer composition of 4HB also varied from 4 to 55 mol% when
alkanediols with even carbon numbers such as 1,4-butanediol, 1,6-hexanediol, 1,8-
octanediol, 1,10-decanediol and 1,12-dodecanediol were used. Feeding strategy of
interval feeding time could increased the cell dry weight up to 30 and 31.1 g/L according
to 12 and 6 h interval of feeding time respectively, but the 4HB compositions unable to
be varied. Feeding of C/N 5 at growth phase and supplementing different concentrations
of γ-butyrolactone in accumulation phase using DO-stat method had successfully
produced various 4HB compositions ranging from 35 to 68 mol% with the cell dry
weight increased up to 14.9 g/L. |
| first_indexed | 2024-03-06T15:21:12Z |
| format | Thesis |
| id | usm.eprints-40936 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T15:21:12Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | usm.eprints-409362018-07-06T07:43:34Z http://eprints.usm.my/40936/ Production And Characterization Of Poly(3-Hydroxybutyrate-Co-4-Hydroxybutyrate) Copolymer Ahmad, Rahayu QH1 Natural history (General - Including nature conservation, geographical distribution) A locally isolated Cupriavidus sp. USMAA2-4 possessing the ability to produce biodegradable polymer was used in this study to explore the production of biopolymer through one-stage cultivation process and fed-batch fermentation. Various palm oilbased carbon sources, fatty acids and its combination were used for producing high cell dry weight of Cupriavidus sp. USMAA2-4. Utilization of oleic acid solely yielding the highest cell dry weight with 8.4 g/L. Effects of carbon to nitrogen (C/N ratio) on the accumulation of P(3HB) before feeding of carbon precursor (γ-butyrolactone) exhibited significant increased of 4HB compositions from 9 to 55 mol% when C/N ratio decreased from 20-8. The monomer composition of 4HB also varied from 4 to 55 mol% when alkanediols with even carbon numbers such as 1,4-butanediol, 1,6-hexanediol, 1,8- octanediol, 1,10-decanediol and 1,12-dodecanediol were used. Feeding strategy of interval feeding time could increased the cell dry weight up to 30 and 31.1 g/L according to 12 and 6 h interval of feeding time respectively, but the 4HB compositions unable to be varied. Feeding of C/N 5 at growth phase and supplementing different concentrations of γ-butyrolactone in accumulation phase using DO-stat method had successfully produced various 4HB compositions ranging from 35 to 68 mol% with the cell dry weight increased up to 14.9 g/L. 2011-07 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/40936/1/Rahayu_bt_Ahmad_24MS_HJ.pdf Ahmad, Rahayu (2011) Production And Characterization Of Poly(3-Hydroxybutyrate-Co-4-Hydroxybutyrate) Copolymer. Masters thesis, Universiti Sains Malaysia. |
| spellingShingle | QH1 Natural history (General - Including nature conservation, geographical distribution) Ahmad, Rahayu Production And Characterization Of Poly(3-Hydroxybutyrate-Co-4-Hydroxybutyrate) Copolymer |
| title | Production And Characterization Of
Poly(3-Hydroxybutyrate-Co-4-Hydroxybutyrate)
Copolymer
|
| title_full | Production And Characterization Of
Poly(3-Hydroxybutyrate-Co-4-Hydroxybutyrate)
Copolymer
|
| title_fullStr | Production And Characterization Of
Poly(3-Hydroxybutyrate-Co-4-Hydroxybutyrate)
Copolymer
|
| title_full_unstemmed | Production And Characterization Of
Poly(3-Hydroxybutyrate-Co-4-Hydroxybutyrate)
Copolymer
|
| title_short | Production And Characterization Of
Poly(3-Hydroxybutyrate-Co-4-Hydroxybutyrate)
Copolymer
|
| title_sort | production and characterization of poly 3 hydroxybutyrate co 4 hydroxybutyrate copolymer |
| topic | QH1 Natural history (General - Including nature conservation, geographical distribution) |
| url | http://eprints.usm.my/40936/1/Rahayu_bt_Ahmad_24MS_HJ.pdf |
| work_keys_str_mv | AT ahmadrahayu productionandcharacterizationofpoly3hydroxybutyrateco4hydroxybutyratecopolymer |