Cloning And Expression Of Synthetic Human Erythropoietin In Methylotrophic Yeast, Pichia Pastoris
Human erythropoietin is a hormone or glycoprotein produced by the kidney and bone marrow with important physiological functions, such as, erythropoiesis, angiogenesis, and wound healing. The natural source of huEpo is purified from human urine (uhuEPO). Alternatively, recombinant human erythropoi...
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| Format: | Thesis |
| Language: | English |
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2010
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| Online Access: | http://eprints.usm.my/41239/1/Yazmin_Bustami-Shahfiq.pdf |
| _version_ | 1797010593233764352 |
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| author | Bustami, Yazmin |
| author_facet | Bustami, Yazmin |
| author_sort | Bustami, Yazmin |
| collection | USM |
| description | Human erythropoietin is a hormone or glycoprotein produced by the kidney
and bone marrow with important physiological functions, such as, erythropoiesis,
angiogenesis, and wound healing. The natural source of huEpo is purified from
human urine (uhuEPO). Alternatively, recombinant human erythropoietin could
overcome the limited supply of uhuEPO. Thus, the focus of this study is to generate a
synthetic huEPO gene and express in Pichia pastoris. PCR assembly method utilized
to construct a synthetic gene using twenty sets of overlapping oligonucleotides,
covering the huEPO A gene sequence and two newly introduced restriction enzyme
sites. Results from DNA sequence analysis showed accurate assembled synthetic
huEPO gene albeit minor base mutations detected. |
| first_indexed | 2024-03-06T15:22:04Z |
| format | Thesis |
| id | usm.eprints-41239 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T15:22:04Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | usm.eprints-412392018-08-06T07:38:58Z http://eprints.usm.my/41239/ Cloning And Expression Of Synthetic Human Erythropoietin In Methylotrophic Yeast, Pichia Pastoris Bustami, Yazmin QH1 Natural history (General - Including nature conservation, geographical distribution) Human erythropoietin is a hormone or glycoprotein produced by the kidney and bone marrow with important physiological functions, such as, erythropoiesis, angiogenesis, and wound healing. The natural source of huEpo is purified from human urine (uhuEPO). Alternatively, recombinant human erythropoietin could overcome the limited supply of uhuEPO. Thus, the focus of this study is to generate a synthetic huEPO gene and express in Pichia pastoris. PCR assembly method utilized to construct a synthetic gene using twenty sets of overlapping oligonucleotides, covering the huEPO A gene sequence and two newly introduced restriction enzyme sites. Results from DNA sequence analysis showed accurate assembled synthetic huEPO gene albeit minor base mutations detected. 2010-11 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/41239/1/Yazmin_Bustami-Shahfiq.pdf Bustami, Yazmin (2010) Cloning And Expression Of Synthetic Human Erythropoietin In Methylotrophic Yeast, Pichia Pastoris. Masters thesis, Universiti Sains Malaysia. |
| spellingShingle | QH1 Natural history (General - Including nature conservation, geographical distribution) Bustami, Yazmin Cloning And Expression Of Synthetic Human Erythropoietin In Methylotrophic Yeast, Pichia Pastoris |
| title | Cloning And Expression Of Synthetic Human
Erythropoietin In Methylotrophic Yeast, Pichia Pastoris
|
| title_full | Cloning And Expression Of Synthetic Human
Erythropoietin In Methylotrophic Yeast, Pichia Pastoris
|
| title_fullStr | Cloning And Expression Of Synthetic Human
Erythropoietin In Methylotrophic Yeast, Pichia Pastoris
|
| title_full_unstemmed | Cloning And Expression Of Synthetic Human
Erythropoietin In Methylotrophic Yeast, Pichia Pastoris
|
| title_short | Cloning And Expression Of Synthetic Human
Erythropoietin In Methylotrophic Yeast, Pichia Pastoris
|
| title_sort | cloning and expression of synthetic human erythropoietin in methylotrophic yeast pichia pastoris |
| topic | QH1 Natural history (General - Including nature conservation, geographical distribution) |
| url | http://eprints.usm.my/41239/1/Yazmin_Bustami-Shahfiq.pdf |
| work_keys_str_mv | AT bustamiyazmin cloningandexpressionofsynthetichumanerythropoietininmethylotrophicyeastpichiapastoris |