Development Of DNA-Based Diagnostic Tests For The Detection Of Shigella Dysenteriae, Shigella Flexneri And Shigella Sonnei
Shigellosis or bacillary dysentery caused by Shigella spp. remains as significant health problem worldwide. Current detection and identification methods of the pathogens from stool specimens by culture method is relatively insensitive, generally time consuming (~ 2-5 days) and laborious. In this stu...
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| Format: | Thesis |
| Language: | English |
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2010
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| Online Access: | http://eprints.usm.my/42167/1/NIK_NOORUL_SHAKIRA.pdf |
| _version_ | 1825834565965447168 |
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| author | Shakrin, Nik Noorul Shakira Mohamed |
| author_facet | Shakrin, Nik Noorul Shakira Mohamed |
| author_sort | Shakrin, Nik Noorul Shakira Mohamed |
| collection | USM |
| description | Shigellosis or bacillary dysentery caused by Shigella spp. remains as significant health problem worldwide. Current detection and identification methods of the pathogens from stool specimens by culture method is relatively insensitive, generally time consuming (~ 2-5 days) and laborious. In this study, thermostabilised Polymerase Chain Reaction (PCR) assays were developed for the detection of ompA genes in Shigella dysenteriae, Shigella flexneri and Shigella sonnei. The tests contained specific primers for the detection of S. dysenteriae, S. flexneri and S. sonnei respectively, freezed-dried or thermostabilised PCR reagents with Internal Control (IC) and gel loading dye. |
| first_indexed | 2024-03-06T15:24:40Z |
| format | Thesis |
| id | usm.eprints-42167 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T15:24:40Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | usm.eprints-421672019-04-12T05:26:57Z http://eprints.usm.my/42167/ Development Of DNA-Based Diagnostic Tests For The Detection Of Shigella Dysenteriae, Shigella Flexneri And Shigella Sonnei Shakrin, Nik Noorul Shakira Mohamed R735-854 Medical education. Medical schools. Research Shigellosis or bacillary dysentery caused by Shigella spp. remains as significant health problem worldwide. Current detection and identification methods of the pathogens from stool specimens by culture method is relatively insensitive, generally time consuming (~ 2-5 days) and laborious. In this study, thermostabilised Polymerase Chain Reaction (PCR) assays were developed for the detection of ompA genes in Shigella dysenteriae, Shigella flexneri and Shigella sonnei. The tests contained specific primers for the detection of S. dysenteriae, S. flexneri and S. sonnei respectively, freezed-dried or thermostabilised PCR reagents with Internal Control (IC) and gel loading dye. 2010-03 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/42167/1/NIK_NOORUL_SHAKIRA.pdf Shakrin, Nik Noorul Shakira Mohamed (2010) Development Of DNA-Based Diagnostic Tests For The Detection Of Shigella Dysenteriae, Shigella Flexneri And Shigella Sonnei. Masters thesis, Universiti Sains Malaysia. |
| spellingShingle | R735-854 Medical education. Medical schools. Research Shakrin, Nik Noorul Shakira Mohamed Development Of DNA-Based Diagnostic Tests For The Detection Of Shigella Dysenteriae, Shigella Flexneri And Shigella Sonnei |
| title | Development Of DNA-Based Diagnostic Tests For The Detection Of Shigella Dysenteriae, Shigella Flexneri And Shigella Sonnei |
| title_full | Development Of DNA-Based Diagnostic Tests For The Detection Of Shigella Dysenteriae, Shigella Flexneri And Shigella Sonnei |
| title_fullStr | Development Of DNA-Based Diagnostic Tests For The Detection Of Shigella Dysenteriae, Shigella Flexneri And Shigella Sonnei |
| title_full_unstemmed | Development Of DNA-Based Diagnostic Tests For The Detection Of Shigella Dysenteriae, Shigella Flexneri And Shigella Sonnei |
| title_short | Development Of DNA-Based Diagnostic Tests For The Detection Of Shigella Dysenteriae, Shigella Flexneri And Shigella Sonnei |
| title_sort | development of dna based diagnostic tests for the detection of shigella dysenteriae shigella flexneri and shigella sonnei |
| topic | R735-854 Medical education. Medical schools. Research |
| url | http://eprints.usm.my/42167/1/NIK_NOORUL_SHAKIRA.pdf |
| work_keys_str_mv | AT shakrinniknoorulshakiramohamed developmentofdnabaseddiagnostictestsforthedetectionofshigelladysenteriaeshigellaflexneriandshigellasonnei |