The Cyto-Genotoxicity Evaluation Of Biomedical Grade Chitosan On Primary Keratinocyte Cultures And Its Role In Scar Formation Pathways Using Skin Coculture Model
Chitosan (-1, 4—D-glucosamine) is a deacetylated form of chitin with excellent biological properties useful in wound management. However, improvement of the physical and chemical properties of the chitosan will alter its biocompatibility. Additionally, chitosan has been reported to be useful in...
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| Format: | Thesis |
| Language: | English |
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2010
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| Online Access: | http://eprints.usm.my/42288/1/Lim_Chin_Keong24.pdf |
| _version_ | 1797010797816184832 |
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| author | Lim , Chin Keong |
| author_facet | Lim , Chin Keong |
| author_sort | Lim , Chin Keong |
| collection | USM |
| description | Chitosan (-1, 4—D-glucosamine) is a deacetylated form of chitin with excellent
biological properties useful in wound management. However, improvement of the
physical and chemical properties of the chitosan will alter its biocompatibility.
Additionally, chitosan has been reported to be useful in scar intervention. Therefore in
this study, the in vitro biocompatibility of the modified chitosan porous skin
regenerating templates (CPSRTs) has been evaluated based on cytotoxicity analysis by
3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay,
genotoxicity by comet assay, and skin keratinocyte pro-inflammatory cytokine
secretions [interleukin-1 (IL-1), tumor necrosis factor- (TNF-) and interleukin-8
(IL-8)], using primary normal human epidermal keratinocyte (pNHEK) cultures. The
most biocompatible CPSRT was then investigated for its role in keloid intervention
using keratinocyte-fibroblast cocultures. The human collagen type-I, III and V protein
expressions were assayed in Western blot analysis. |
| first_indexed | 2024-03-06T15:25:01Z |
| format | Thesis |
| id | usm.eprints-42288 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T15:25:01Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | usm.eprints-422882019-04-12T05:26:51Z http://eprints.usm.my/42288/ The Cyto-Genotoxicity Evaluation Of Biomedical Grade Chitosan On Primary Keratinocyte Cultures And Its Role In Scar Formation Pathways Using Skin Coculture Model Lim , Chin Keong R5-920 Medicine (General) Chitosan (-1, 4—D-glucosamine) is a deacetylated form of chitin with excellent biological properties useful in wound management. However, improvement of the physical and chemical properties of the chitosan will alter its biocompatibility. Additionally, chitosan has been reported to be useful in scar intervention. Therefore in this study, the in vitro biocompatibility of the modified chitosan porous skin regenerating templates (CPSRTs) has been evaluated based on cytotoxicity analysis by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay, genotoxicity by comet assay, and skin keratinocyte pro-inflammatory cytokine secretions [interleukin-1 (IL-1), tumor necrosis factor- (TNF-) and interleukin-8 (IL-8)], using primary normal human epidermal keratinocyte (pNHEK) cultures. The most biocompatible CPSRT was then investigated for its role in keloid intervention using keratinocyte-fibroblast cocultures. The human collagen type-I, III and V protein expressions were assayed in Western blot analysis. 2010-06 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/42288/1/Lim_Chin_Keong24.pdf Lim , Chin Keong (2010) The Cyto-Genotoxicity Evaluation Of Biomedical Grade Chitosan On Primary Keratinocyte Cultures And Its Role In Scar Formation Pathways Using Skin Coculture Model. PhD thesis, Universiti Sains Malaysia. |
| spellingShingle | R5-920 Medicine (General) Lim , Chin Keong The Cyto-Genotoxicity Evaluation Of Biomedical Grade Chitosan On Primary Keratinocyte Cultures And Its Role In Scar Formation Pathways Using Skin Coculture Model |
| title | The Cyto-Genotoxicity Evaluation Of Biomedical Grade
Chitosan On Primary Keratinocyte Cultures And Its Role In
Scar Formation Pathways Using Skin Coculture Model
|
| title_full | The Cyto-Genotoxicity Evaluation Of Biomedical Grade
Chitosan On Primary Keratinocyte Cultures And Its Role In
Scar Formation Pathways Using Skin Coculture Model
|
| title_fullStr | The Cyto-Genotoxicity Evaluation Of Biomedical Grade
Chitosan On Primary Keratinocyte Cultures And Its Role In
Scar Formation Pathways Using Skin Coculture Model
|
| title_full_unstemmed | The Cyto-Genotoxicity Evaluation Of Biomedical Grade
Chitosan On Primary Keratinocyte Cultures And Its Role In
Scar Formation Pathways Using Skin Coculture Model
|
| title_short | The Cyto-Genotoxicity Evaluation Of Biomedical Grade
Chitosan On Primary Keratinocyte Cultures And Its Role In
Scar Formation Pathways Using Skin Coculture Model
|
| title_sort | cyto genotoxicity evaluation of biomedical grade chitosan on primary keratinocyte cultures and its role in scar formation pathways using skin coculture model |
| topic | R5-920 Medicine (General) |
| url | http://eprints.usm.my/42288/1/Lim_Chin_Keong24.pdf |
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