Analysis Of Proteins Extracted From The Leaves Of Piper Sarmentosum
A gel based proteomic method has been developed for the leaf tissues of P. sarmentosum. A suitable protein extraction method termed as total protein extraction followed by trichloroacetic acid (TCA)/acetone precipitation was applied and has been shown to produce good leaf tissue extract that was com...
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| Format: | Thesis |
| Language: | English |
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2010
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| Online Access: | http://eprints.usm.my/42578/1/SHIM_SIANG_YIAN.pdf |
| _version_ | 1797010857201238016 |
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| author | Shim , Siang yian |
| author_facet | Shim , Siang yian |
| author_sort | Shim , Siang yian |
| collection | USM |
| description | A gel based proteomic method has been developed for the leaf tissues of P. sarmentosum. A suitable protein extraction method termed as total protein extraction followed by trichloroacetic acid (TCA)/acetone precipitation was applied and has been shown to produce good leaf tissue extract that was compatible with 2-DE separation. The protein profiling of leaf tissues of P. sarmentosum showed a total of 242 protein spots and from which 283 proteins were identified by LCMS/MS analysis. Most of these identified proteins were involved in metabolism (including photosynthesis), protein destination and storage, energy and transport, and defense and stress related protein. |
| first_indexed | 2024-03-06T15:25:49Z |
| format | Thesis |
| id | usm.eprints-42578 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T15:25:49Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | usm.eprints-425782019-04-12T05:26:47Z http://eprints.usm.my/42578/ Analysis Of Proteins Extracted From The Leaves Of Piper Sarmentosum Shim , Siang yian TP1-1185 Chemical technology A gel based proteomic method has been developed for the leaf tissues of P. sarmentosum. A suitable protein extraction method termed as total protein extraction followed by trichloroacetic acid (TCA)/acetone precipitation was applied and has been shown to produce good leaf tissue extract that was compatible with 2-DE separation. The protein profiling of leaf tissues of P. sarmentosum showed a total of 242 protein spots and from which 283 proteins were identified by LCMS/MS analysis. Most of these identified proteins were involved in metabolism (including photosynthesis), protein destination and storage, energy and transport, and defense and stress related protein. 2010-10 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/42578/1/SHIM_SIANG_YIAN.pdf Shim , Siang yian (2010) Analysis Of Proteins Extracted From The Leaves Of Piper Sarmentosum. Masters thesis, Universiti Sains Malaysia. |
| spellingShingle | TP1-1185 Chemical technology Shim , Siang yian Analysis Of Proteins Extracted From The Leaves Of Piper Sarmentosum |
| title | Analysis Of Proteins Extracted From The Leaves Of Piper Sarmentosum
|
| title_full | Analysis Of Proteins Extracted From The Leaves Of Piper Sarmentosum
|
| title_fullStr | Analysis Of Proteins Extracted From The Leaves Of Piper Sarmentosum
|
| title_full_unstemmed | Analysis Of Proteins Extracted From The Leaves Of Piper Sarmentosum
|
| title_short | Analysis Of Proteins Extracted From The Leaves Of Piper Sarmentosum
|
| title_sort | analysis of proteins extracted from the leaves of piper sarmentosum |
| topic | TP1-1185 Chemical technology |
| url | http://eprints.usm.my/42578/1/SHIM_SIANG_YIAN.pdf |
| work_keys_str_mv | AT shimsiangyian analysisofproteinsextractedfromtheleavesofpipersarmentosum |