Design, Fabrication And Characterization Of Fr4-Based Dna Label Free Sensor Integrated With Pocket-Sized Readout Circuitry
Silicon, glass and ceramic are commonly base substrates used in SU8 film fabrication and DNA biosensor. All these substrates are biocompatible, expensive, hard and brittle, difficult for drilling and dicing, requires expensive equipments and complex methodology of fabrication. Microcracks are oft...
| Main Author: | |
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| Format: | Thesis |
| Language: | English |
| Published: |
2013
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| Subjects: | |
| Online Access: | http://eprints.usm.my/43591/1/Irni%20Hamiza%20Binti%20Hamzah24.pdf |
| _version_ | 1825834819454500864 |
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| author | Hamzah, Irni Hamiza |
| author_facet | Hamzah, Irni Hamiza |
| author_sort | Hamzah, Irni Hamiza |
| collection | USM |
| description | Silicon, glass and ceramic are commonly base substrates used in SU8 film
fabrication and DNA biosensor. All these substrates are biocompatible, expensive, hard
and brittle, difficult for drilling and dicing, requires expensive equipments and complex
methodology of fabrication. Microcracks are often found on SU8 film due to the large
gap of thermal expansion coefficient (TEC) between silicon/glass and SU8. In addition,
the use of bulky equipments and accessories that must be interfaced with hardware
and software through a central processing unit (CPU) limits the portability of the whole
biosensor system. Hence, the aim of this work is to investigate on the suitability of
polymethylmethacrylate (PMMA) to be used as a base substrate for SU8 film
fabrication and the application of a non-biocompatible material, flame retardant 4 (FR4)
as a base substrate for a label free DNA biosensor. A portable and handy readout
circuitry with double-sided pocket-sized of PCB for DNA detection through current
measurement cyclic voltammetry (CV) method has been developed and integrated with
the FR4-based fabricated sensor. The Au is fabricated throughout all conducting tracks
on the FR4 and the characterization of the fabricated FR4-based sensor to perform CV
reversible process is confirmed by a series of analysis. The thiol group at the 3’-end of
ssDNA is used to link the DNA with the Au surface and act as probe DNAs. Two types
of target DNAs which consist of complementary nucleobase sequence and
noncomplementary nucleobase sequence are used to investigate on the hybridization
mechanism with the probe DNAs. |
| first_indexed | 2024-03-06T15:28:25Z |
| format | Thesis |
| id | usm.eprints-43591 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T15:28:25Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | usm.eprints-435912019-04-12T05:26:13Z http://eprints.usm.my/43591/ Design, Fabrication And Characterization Of Fr4-Based Dna Label Free Sensor Integrated With Pocket-Sized Readout Circuitry Hamzah, Irni Hamiza TK1-9971 Electrical engineering. Electronics. Nuclear engineering Silicon, glass and ceramic are commonly base substrates used in SU8 film fabrication and DNA biosensor. All these substrates are biocompatible, expensive, hard and brittle, difficult for drilling and dicing, requires expensive equipments and complex methodology of fabrication. Microcracks are often found on SU8 film due to the large gap of thermal expansion coefficient (TEC) between silicon/glass and SU8. In addition, the use of bulky equipments and accessories that must be interfaced with hardware and software through a central processing unit (CPU) limits the portability of the whole biosensor system. Hence, the aim of this work is to investigate on the suitability of polymethylmethacrylate (PMMA) to be used as a base substrate for SU8 film fabrication and the application of a non-biocompatible material, flame retardant 4 (FR4) as a base substrate for a label free DNA biosensor. A portable and handy readout circuitry with double-sided pocket-sized of PCB for DNA detection through current measurement cyclic voltammetry (CV) method has been developed and integrated with the FR4-based fabricated sensor. The Au is fabricated throughout all conducting tracks on the FR4 and the characterization of the fabricated FR4-based sensor to perform CV reversible process is confirmed by a series of analysis. The thiol group at the 3’-end of ssDNA is used to link the DNA with the Au surface and act as probe DNAs. Two types of target DNAs which consist of complementary nucleobase sequence and noncomplementary nucleobase sequence are used to investigate on the hybridization mechanism with the probe DNAs. 2013-06 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/43591/1/Irni%20Hamiza%20Binti%20Hamzah24.pdf Hamzah, Irni Hamiza (2013) Design, Fabrication And Characterization Of Fr4-Based Dna Label Free Sensor Integrated With Pocket-Sized Readout Circuitry. PhD thesis, Universiti Sains Malaysia. |
| spellingShingle | TK1-9971 Electrical engineering. Electronics. Nuclear engineering Hamzah, Irni Hamiza Design, Fabrication And Characterization Of Fr4-Based Dna Label Free Sensor Integrated With Pocket-Sized Readout Circuitry |
| title | Design, Fabrication And Characterization Of
Fr4-Based Dna Label Free Sensor Integrated With
Pocket-Sized Readout Circuitry |
| title_full | Design, Fabrication And Characterization Of
Fr4-Based Dna Label Free Sensor Integrated With
Pocket-Sized Readout Circuitry |
| title_fullStr | Design, Fabrication And Characterization Of
Fr4-Based Dna Label Free Sensor Integrated With
Pocket-Sized Readout Circuitry |
| title_full_unstemmed | Design, Fabrication And Characterization Of
Fr4-Based Dna Label Free Sensor Integrated With
Pocket-Sized Readout Circuitry |
| title_short | Design, Fabrication And Characterization Of
Fr4-Based Dna Label Free Sensor Integrated With
Pocket-Sized Readout Circuitry |
| title_sort | design fabrication and characterization of fr4 based dna label free sensor integrated with pocket sized readout circuitry |
| topic | TK1-9971 Electrical engineering. Electronics. Nuclear engineering |
| url | http://eprints.usm.my/43591/1/Irni%20Hamiza%20Binti%20Hamzah24.pdf |
| work_keys_str_mv | AT hamzahirnihamiza designfabricationandcharacterizationoffr4baseddnalabelfreesensorintegratedwithpocketsizedreadoutcircuitry |