Phytochemical Screening and Cytotoxic Effects of Crude Extracts of Pereskia Bleo Leaves
The phytocompounds in crude solvent extracts of Pereskia bleo leaves were identified and their cytotoxic effects on cancer cell lines were determined. Crude extracts were obtained via maceration and subjected to GCMS analysis. Then, each extract was incubated with HeLa, MDA-MB-231, SW480, and NI...
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| Format: | Article |
| Language: | English |
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Taylor & Francis
2020
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| Online Access: | http://eprints.usm.my/44086/1/Phytochemical%20Screening%20and%20Cytotoxic%20Effects%20of%20Crude%20Extracts%20of%20Pereskia%20Bleo%20Leaves%20%281%29.pdf |
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| author | Salleh, Siti Farhanah Mohd Ismail, Norzila Ibrahim, Wan Suriyani Wan Ismail, Tuan Nadrah Naim Tuan |
| author_facet | Salleh, Siti Farhanah Mohd Ismail, Norzila Ibrahim, Wan Suriyani Wan Ismail, Tuan Nadrah Naim Tuan |
| author_sort | Salleh, Siti Farhanah Mohd |
| collection | USM |
| description | The phytocompounds in crude solvent extracts of Pereskia bleo
leaves were identified and their cytotoxic effects on cancer cell
lines were determined. Crude extracts were obtained via
maceration and subjected to GCMS analysis. Then, each extract
was incubated with HeLa, MDA-MB-231, SW480, and NIH/3T3
cell lines for 72 h. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide assay was done to determine IC50 values
of each extract. Terpenoids, sterols, alkaloids, fatty acids and
phenolic compounds were identified from the crude extracts
of P. bleo leaves. Other compounds identified were γ-sitosterol,
β-tocopherol, and γ-tocopherol. The ethyl acetate extract had
potent cytotoxic effect against HeLa and MDA-MB-231 cancer
cells as noted by the lowest IC50 values |
| first_indexed | 2024-03-06T15:29:43Z |
| format | Article |
| id | usm.eprints-44086 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T15:29:43Z |
| publishDate | 2020 |
| publisher | Taylor & Francis |
| record_format | dspace |
| spelling | usm.eprints-440862020-02-28T07:52:24Z http://eprints.usm.my/44086/ Phytochemical Screening and Cytotoxic Effects of Crude Extracts of Pereskia Bleo Leaves Salleh, Siti Farhanah Mohd Ismail, Norzila Ibrahim, Wan Suriyani Wan Ismail, Tuan Nadrah Naim Tuan RC254-282 Neoplasms. Tumors. Oncology (including Cancer) The phytocompounds in crude solvent extracts of Pereskia bleo leaves were identified and their cytotoxic effects on cancer cell lines were determined. Crude extracts were obtained via maceration and subjected to GCMS analysis. Then, each extract was incubated with HeLa, MDA-MB-231, SW480, and NIH/3T3 cell lines for 72 h. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was done to determine IC50 values of each extract. Terpenoids, sterols, alkaloids, fatty acids and phenolic compounds were identified from the crude extracts of P. bleo leaves. Other compounds identified were γ-sitosterol, β-tocopherol, and γ-tocopherol. The ethyl acetate extract had potent cytotoxic effect against HeLa and MDA-MB-231 cancer cells as noted by the lowest IC50 values Taylor & Francis 2020 Article PeerReviewed application/pdf en http://eprints.usm.my/44086/1/Phytochemical%20Screening%20and%20Cytotoxic%20Effects%20of%20Crude%20Extracts%20of%20Pereskia%20Bleo%20Leaves%20%281%29.pdf Salleh, Siti Farhanah Mohd and Ismail, Norzila and Ibrahim, Wan Suriyani Wan and Ismail, Tuan Nadrah Naim Tuan (2020) Phytochemical Screening and Cytotoxic Effects of Crude Extracts of Pereskia Bleo Leaves. Journal of Herbs, Spices and Medicinal Plants. ISSN 1540-3580 (Submitted) https://www.tandfonline.com/doi/full/10.1080/10496475.2020.1729287 |
| spellingShingle | RC254-282 Neoplasms. Tumors. Oncology (including Cancer) Salleh, Siti Farhanah Mohd Ismail, Norzila Ibrahim, Wan Suriyani Wan Ismail, Tuan Nadrah Naim Tuan Phytochemical Screening and Cytotoxic Effects of Crude Extracts of Pereskia Bleo Leaves |
| title | Phytochemical Screening and Cytotoxic Effects of
Crude Extracts of Pereskia Bleo Leaves |
| title_full | Phytochemical Screening and Cytotoxic Effects of
Crude Extracts of Pereskia Bleo Leaves |
| title_fullStr | Phytochemical Screening and Cytotoxic Effects of
Crude Extracts of Pereskia Bleo Leaves |
| title_full_unstemmed | Phytochemical Screening and Cytotoxic Effects of
Crude Extracts of Pereskia Bleo Leaves |
| title_short | Phytochemical Screening and Cytotoxic Effects of
Crude Extracts of Pereskia Bleo Leaves |
| title_sort | phytochemical screening and cytotoxic effects of crude extracts of pereskia bleo leaves |
| topic | RC254-282 Neoplasms. Tumors. Oncology (including Cancer) |
| url | http://eprints.usm.my/44086/1/Phytochemical%20Screening%20and%20Cytotoxic%20Effects%20of%20Crude%20Extracts%20of%20Pereskia%20Bleo%20Leaves%20%281%29.pdf |
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