Agrobacterium-Mediated Transformation Of Dendrobium Sonia-28
Effective propagation of protocorm-like bodies (PLBs) of Dendrobium sonia-28 was achieved by using micropropagation technique. The highest PLBs proliferation within 21 days was observed in semi-solid media over liquid culture. Half strength of Murashige and Skoog (MS) media produced the highest PLBs...
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| Format: | Thesis |
| Language: | English |
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2012
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| Online Access: | http://eprints.usm.my/44776/1/ADVINA%20LIZAH%20BINTI%20JULKIFLE.pdf |
| _version_ | 1825835030803382272 |
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| author | Julkifle, Advina Lizah |
| author_facet | Julkifle, Advina Lizah |
| author_sort | Julkifle, Advina Lizah |
| collection | USM |
| description | Effective propagation of protocorm-like bodies (PLBs) of Dendrobium sonia-28 was achieved by using micropropagation technique. The highest PLBs proliferation within 21 days was observed in semi-solid media over liquid culture. Half strength of Murashige and Skoog (MS) media produced the highest PLBs growth rate percentage compared to full and double strength MS media in semi-solid and liquid cultures. In sucrose treatment, the highest PLBs growth rate percentage was in 10 g.L-1 sucrose. In the conditions of absence of sucrose (0 g.L-1) or excessive of sucrose (30 g.L-1) had proven detrimental for the PLBs growth and development. |
| first_indexed | 2024-03-06T15:31:36Z |
| format | Thesis |
| id | usm.eprints-44776 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T15:31:36Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | usm.eprints-447762019-07-01T02:11:17Z http://eprints.usm.my/44776/ Agrobacterium-Mediated Transformation Of Dendrobium Sonia-28 Julkifle, Advina Lizah QH1 Natural history (General - Including nature conservation, geographical distribution) Effective propagation of protocorm-like bodies (PLBs) of Dendrobium sonia-28 was achieved by using micropropagation technique. The highest PLBs proliferation within 21 days was observed in semi-solid media over liquid culture. Half strength of Murashige and Skoog (MS) media produced the highest PLBs growth rate percentage compared to full and double strength MS media in semi-solid and liquid cultures. In sucrose treatment, the highest PLBs growth rate percentage was in 10 g.L-1 sucrose. In the conditions of absence of sucrose (0 g.L-1) or excessive of sucrose (30 g.L-1) had proven detrimental for the PLBs growth and development. 2012-06 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/44776/1/ADVINA%20LIZAH%20BINTI%20JULKIFLE.pdf Julkifle, Advina Lizah (2012) Agrobacterium-Mediated Transformation Of Dendrobium Sonia-28. Masters thesis, Universiti Sains Malaysia. |
| spellingShingle | QH1 Natural history (General - Including nature conservation, geographical distribution) Julkifle, Advina Lizah Agrobacterium-Mediated Transformation Of Dendrobium Sonia-28 |
| title | Agrobacterium-Mediated Transformation Of Dendrobium Sonia-28 |
| title_full | Agrobacterium-Mediated Transformation Of Dendrobium Sonia-28 |
| title_fullStr | Agrobacterium-Mediated Transformation Of Dendrobium Sonia-28 |
| title_full_unstemmed | Agrobacterium-Mediated Transformation Of Dendrobium Sonia-28 |
| title_short | Agrobacterium-Mediated Transformation Of Dendrobium Sonia-28 |
| title_sort | agrobacterium mediated transformation of dendrobium sonia 28 |
| topic | QH1 Natural history (General - Including nature conservation, geographical distribution) |
| url | http://eprints.usm.my/44776/1/ADVINA%20LIZAH%20BINTI%20JULKIFLE.pdf |
| work_keys_str_mv | AT julkifleadvinalizah agrobacteriummediatedtransformationofdendrobiumsonia28 |