Proteomic Analysis Of Prostate Carcinogenesis Induced By Heterocyclic Amine 2-Amino-1-Methyl- 6-Phenylimidazo[4,5-B]Pyridine (PhIP) Using In Vitro Model
2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) is the most abundant type of carcinogenic heterocyclic amine produced in cooked meat. This study aims to investigate the carcinogenic effect of human physiologically relevant concentration of PhIP on human prostate epithelial cell line, RWPE-1,...
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| Format: | Thesis |
| Language: | English |
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2019
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| Online Access: | http://eprints.usm.my/45071/1/SITI%20NAZMIN%20SAIFUDDIN.pdf |
| _version_ | 1797011308226281472 |
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| author | Saifuddin, Siti Nazmin |
| author_facet | Saifuddin, Siti Nazmin |
| author_sort | Saifuddin, Siti Nazmin |
| collection | USM |
| description | 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) is the most abundant type of carcinogenic heterocyclic amine produced in cooked meat. This study aims to investigate the carcinogenic effect of human physiologically relevant
concentration of PhIP on human prostate epithelial cell line, RWPE-1, at cellular and protein levels in prostate carcinogenesis. RWPE-1 cells were exposed to 10-7, 10-8,
10-9 or 10-10 M PhIP, diluted in 0.003%, 0.025% or 0.25% DMSO, with or without metabolic activation system. The methodologies used in this study were MTS assay for cytotoxicity and cell proliferation tests, Alamar Blue assay for cell proliferation test, cell transformation assay, invasion assay and shotgun proteomic. |
| first_indexed | 2024-03-06T15:32:26Z |
| format | Thesis |
| id | usm.eprints-45071 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T15:32:26Z |
| publishDate | 2019 |
| record_format | dspace |
| spelling | usm.eprints-450712019-07-26T01:08:51Z http://eprints.usm.my/45071/ Proteomic Analysis Of Prostate Carcinogenesis Induced By Heterocyclic Amine 2-Amino-1-Methyl- 6-Phenylimidazo[4,5-B]Pyridine (PhIP) Using In Vitro Model Saifuddin, Siti Nazmin R735-854 Medical education. Medical schools. Research 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) is the most abundant type of carcinogenic heterocyclic amine produced in cooked meat. This study aims to investigate the carcinogenic effect of human physiologically relevant concentration of PhIP on human prostate epithelial cell line, RWPE-1, at cellular and protein levels in prostate carcinogenesis. RWPE-1 cells were exposed to 10-7, 10-8, 10-9 or 10-10 M PhIP, diluted in 0.003%, 0.025% or 0.25% DMSO, with or without metabolic activation system. The methodologies used in this study were MTS assay for cytotoxicity and cell proliferation tests, Alamar Blue assay for cell proliferation test, cell transformation assay, invasion assay and shotgun proteomic. 2019-01 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/45071/1/SITI%20NAZMIN%20SAIFUDDIN.pdf Saifuddin, Siti Nazmin (2019) Proteomic Analysis Of Prostate Carcinogenesis Induced By Heterocyclic Amine 2-Amino-1-Methyl- 6-Phenylimidazo[4,5-B]Pyridine (PhIP) Using In Vitro Model. PhD thesis, Universiti Sains Malaysia. |
| spellingShingle | R735-854 Medical education. Medical schools. Research Saifuddin, Siti Nazmin Proteomic Analysis Of Prostate Carcinogenesis Induced By Heterocyclic Amine 2-Amino-1-Methyl- 6-Phenylimidazo[4,5-B]Pyridine (PhIP) Using In Vitro Model |
| title | Proteomic Analysis Of Prostate Carcinogenesis Induced By Heterocyclic Amine 2-Amino-1-Methyl- 6-Phenylimidazo[4,5-B]Pyridine (PhIP) Using In Vitro Model |
| title_full | Proteomic Analysis Of Prostate Carcinogenesis Induced By Heterocyclic Amine 2-Amino-1-Methyl- 6-Phenylimidazo[4,5-B]Pyridine (PhIP) Using In Vitro Model |
| title_fullStr | Proteomic Analysis Of Prostate Carcinogenesis Induced By Heterocyclic Amine 2-Amino-1-Methyl- 6-Phenylimidazo[4,5-B]Pyridine (PhIP) Using In Vitro Model |
| title_full_unstemmed | Proteomic Analysis Of Prostate Carcinogenesis Induced By Heterocyclic Amine 2-Amino-1-Methyl- 6-Phenylimidazo[4,5-B]Pyridine (PhIP) Using In Vitro Model |
| title_short | Proteomic Analysis Of Prostate Carcinogenesis Induced By Heterocyclic Amine 2-Amino-1-Methyl- 6-Phenylimidazo[4,5-B]Pyridine (PhIP) Using In Vitro Model |
| title_sort | proteomic analysis of prostate carcinogenesis induced by heterocyclic amine 2 amino 1 methyl 6 phenylimidazo 4 5 b pyridine phip using in vitro model |
| topic | R735-854 Medical education. Medical schools. Research |
| url | http://eprints.usm.my/45071/1/SITI%20NAZMIN%20SAIFUDDIN.pdf |
| work_keys_str_mv | AT saifuddinsitinazmin proteomicanalysisofprostatecarcinogenesisinducedbyheterocyclicamine2amino1methyl6phenylimidazo45bpyridinephipusinginvitromodel |