Synthesis, Characterization, Oxidative Dna Cleavage And Dna Binding Studies Of Mixed Ligands Copper(Ii) Complexes
Six ternary copper(II) complexes of amino acids and polypyridyl ligands with a general formula of [Cu(AA)(B) Cl]·nH2O or [Cu(AA)(B)(H2O)]Cl·nH2O, where AA = DL-threonine, DL-phenylalanine, and DL-valine; B = 2,2’-bipyridyl, 4,4’-dimethyl-2,2’-dipyridyl, and 1,10-phenanthroline are synthesized. The c...
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| Format: | Thesis |
| Language: | English |
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2013
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| Online Access: | http://eprints.usm.my/45216/1/Tan%20Yi%20Han24.pdf |
| _version_ | 1825835110305366016 |
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| author | Tan, Yi Han |
| author_facet | Tan, Yi Han |
| author_sort | Tan, Yi Han |
| collection | USM |
| description | Six ternary copper(II) complexes of amino acids and polypyridyl ligands with a general formula of [Cu(AA)(B) Cl]·nH2O or [Cu(AA)(B)(H2O)]Cl·nH2O, where AA = DL-threonine, DL-phenylalanine, and DL-valine; B = 2,2’-bipyridyl, 4,4’-dimethyl-2,2’-dipyridyl, and 1,10-phenanthroline are synthesized. The complexes are characterized by CHN elemental analysis, AAS, FT-IR, and UV-Vis and have been structurally characterized by X-ray crystallography. All the complexes formed slightly distorted square-pyramidal coordination geometries. The electronic absorption spectra of the complexes show very low intensity of d-d electronic band in the range of 600-635 nm in Tris-HCl/NaCl (5:5 mM) pH 7.2 buffer solution. The DNA binding interaction with CT-DNA has been investigated by electronic absorption spectral titration and viscosity measurements. The results revealed that the phenanthroline complexes interact with CT-DNA through intercalation while bipyridyl complexes bind to CT-DNA through groove binding mode. The calculated intrinsic binding constant (Kb) of all the complexes are in the range of 0.5-6.2 × 105 M-1. All the copper(II) complexes are found to promote efficient DNA cleavage activities at low concentration in the presence of H2O2. The studies show that [Cu(Phe)(Phen)Cl]·3H2O exhibits the highest DNA binding and nuclease activity among the six copper(II) complexes. |
| first_indexed | 2024-03-06T15:32:49Z |
| format | Thesis |
| id | usm.eprints-45216 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T15:32:49Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | usm.eprints-452162019-08-07T06:50:45Z http://eprints.usm.my/45216/ Synthesis, Characterization, Oxidative Dna Cleavage And Dna Binding Studies Of Mixed Ligands Copper(Ii) Complexes Tan, Yi Han TP1-1185 Chemical technology Six ternary copper(II) complexes of amino acids and polypyridyl ligands with a general formula of [Cu(AA)(B) Cl]·nH2O or [Cu(AA)(B)(H2O)]Cl·nH2O, where AA = DL-threonine, DL-phenylalanine, and DL-valine; B = 2,2’-bipyridyl, 4,4’-dimethyl-2,2’-dipyridyl, and 1,10-phenanthroline are synthesized. The complexes are characterized by CHN elemental analysis, AAS, FT-IR, and UV-Vis and have been structurally characterized by X-ray crystallography. All the complexes formed slightly distorted square-pyramidal coordination geometries. The electronic absorption spectra of the complexes show very low intensity of d-d electronic band in the range of 600-635 nm in Tris-HCl/NaCl (5:5 mM) pH 7.2 buffer solution. The DNA binding interaction with CT-DNA has been investigated by electronic absorption spectral titration and viscosity measurements. The results revealed that the phenanthroline complexes interact with CT-DNA through intercalation while bipyridyl complexes bind to CT-DNA through groove binding mode. The calculated intrinsic binding constant (Kb) of all the complexes are in the range of 0.5-6.2 × 105 M-1. All the copper(II) complexes are found to promote efficient DNA cleavage activities at low concentration in the presence of H2O2. The studies show that [Cu(Phe)(Phen)Cl]·3H2O exhibits the highest DNA binding and nuclease activity among the six copper(II) complexes. 2013-07 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/45216/1/Tan%20Yi%20Han24.pdf Tan, Yi Han (2013) Synthesis, Characterization, Oxidative Dna Cleavage And Dna Binding Studies Of Mixed Ligands Copper(Ii) Complexes. Masters thesis, Universiti Sains Malaysia. |
| spellingShingle | TP1-1185 Chemical technology Tan, Yi Han Synthesis, Characterization, Oxidative Dna Cleavage And Dna Binding Studies Of Mixed Ligands Copper(Ii) Complexes |
| title | Synthesis, Characterization, Oxidative Dna Cleavage And Dna Binding Studies Of Mixed Ligands Copper(Ii) Complexes |
| title_full | Synthesis, Characterization, Oxidative Dna Cleavage And Dna Binding Studies Of Mixed Ligands Copper(Ii) Complexes |
| title_fullStr | Synthesis, Characterization, Oxidative Dna Cleavage And Dna Binding Studies Of Mixed Ligands Copper(Ii) Complexes |
| title_full_unstemmed | Synthesis, Characterization, Oxidative Dna Cleavage And Dna Binding Studies Of Mixed Ligands Copper(Ii) Complexes |
| title_short | Synthesis, Characterization, Oxidative Dna Cleavage And Dna Binding Studies Of Mixed Ligands Copper(Ii) Complexes |
| title_sort | synthesis characterization oxidative dna cleavage and dna binding studies of mixed ligands copper ii complexes |
| topic | TP1-1185 Chemical technology |
| url | http://eprints.usm.my/45216/1/Tan%20Yi%20Han24.pdf |
| work_keys_str_mv | AT tanyihan synthesischaracterizationoxidativednacleavageanddnabindingstudiesofmixedligandscopperiicomplexes |