Metabolic Engineering Of Cupriavidus Necator For The Production Of Poly (3-Hydroxybutyrate-Co-3-Hydroxyhexanoate
Polyhydroxyalkanoate (PHA) is a family of polyesters produced by bacteria that possesses physical properties for various industrial applications. In this study, PHA synthase (PhaC) gene isolated from Chromobacterium sp. USM2 (phaCCs USM2) was explored for its potential to synthesize polymer in Cupri...
| Main Author: | |
|---|---|
| Format: | Thesis |
| Language: | English |
| Published: |
2017
|
| Subjects: | |
| Online Access: | http://eprints.usm.my/45355/1/BALQIS%20AB.%20GHANI.pdf |
| _version_ | 1825835136685441024 |
|---|---|
| author | Ab Ghani, Balqis |
| author_facet | Ab Ghani, Balqis |
| author_sort | Ab Ghani, Balqis |
| collection | USM |
| description | Polyhydroxyalkanoate (PHA) is a family of polyesters produced by bacteria that possesses physical properties for various industrial applications. In this study, PHA synthase (PhaC) gene isolated from Chromobacterium sp. USM2 (phaCCs USM2) was explored for its potential to synthesize polymer in Cupriavidus necator. A broad-host range plasmid pBBR1MCS-2 was modified to construct an expression plasmid pYEB-100, which was designed to facilitate cloning of PHA synthases in order to assess the performance of various PhaCs and the composition of PHA accumulated in a standard environment. |
| first_indexed | 2024-03-06T15:33:13Z |
| format | Thesis |
| id | usm.eprints-45355 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T15:33:13Z |
| publishDate | 2017 |
| record_format | dspace |
| spelling | usm.eprints-453552019-09-10T02:45:45Z http://eprints.usm.my/45355/ Metabolic Engineering Of Cupriavidus Necator For The Production Of Poly (3-Hydroxybutyrate-Co-3-Hydroxyhexanoate Ab Ghani, Balqis QH1 Natural history (General - Including nature conservation, geographical distribution) Polyhydroxyalkanoate (PHA) is a family of polyesters produced by bacteria that possesses physical properties for various industrial applications. In this study, PHA synthase (PhaC) gene isolated from Chromobacterium sp. USM2 (phaCCs USM2) was explored for its potential to synthesize polymer in Cupriavidus necator. A broad-host range plasmid pBBR1MCS-2 was modified to construct an expression plasmid pYEB-100, which was designed to facilitate cloning of PHA synthases in order to assess the performance of various PhaCs and the composition of PHA accumulated in a standard environment. 2017-05 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/45355/1/BALQIS%20AB.%20GHANI.pdf Ab Ghani, Balqis (2017) Metabolic Engineering Of Cupriavidus Necator For The Production Of Poly (3-Hydroxybutyrate-Co-3-Hydroxyhexanoate. PhD thesis, Universiti Sains Malaysia. |
| spellingShingle | QH1 Natural history (General - Including nature conservation, geographical distribution) Ab Ghani, Balqis Metabolic Engineering Of Cupriavidus Necator For The Production Of Poly (3-Hydroxybutyrate-Co-3-Hydroxyhexanoate |
| title | Metabolic Engineering Of Cupriavidus Necator For The Production Of Poly (3-Hydroxybutyrate-Co-3-Hydroxyhexanoate |
| title_full | Metabolic Engineering Of Cupriavidus Necator For The Production Of Poly (3-Hydroxybutyrate-Co-3-Hydroxyhexanoate |
| title_fullStr | Metabolic Engineering Of Cupriavidus Necator For The Production Of Poly (3-Hydroxybutyrate-Co-3-Hydroxyhexanoate |
| title_full_unstemmed | Metabolic Engineering Of Cupriavidus Necator For The Production Of Poly (3-Hydroxybutyrate-Co-3-Hydroxyhexanoate |
| title_short | Metabolic Engineering Of Cupriavidus Necator For The Production Of Poly (3-Hydroxybutyrate-Co-3-Hydroxyhexanoate |
| title_sort | metabolic engineering of cupriavidus necator for the production of poly 3 hydroxybutyrate co 3 hydroxyhexanoate |
| topic | QH1 Natural history (General - Including nature conservation, geographical distribution) |
| url | http://eprints.usm.my/45355/1/BALQIS%20AB.%20GHANI.pdf |
| work_keys_str_mv | AT abghanibalqis metabolicengineeringofcupriavidusnecatorfortheproductionofpoly3hydroxybutyrateco3hydroxyhexanoate |