High-Throughput Screening Assay In 384-Well Format For The Identification Of Anti-Trypanosomal Agents Against Trypanosoma Brucei Rhodesiense And Mode Of Cell Death Study
In order to accelerate the discovery of novel leads for the treatment of Human African Trypanosomiasis (HAT), it is necessary to have a simple and sensitive assay to identify positive hits by whole cell viability based high-throughput screening (HTS). In this study, the HTS assay was developed in 38...
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| Format: | Thesis |
| Language: | English |
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2017
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| Online Access: | http://eprints.usm.my/45404/1/LIM%20KAH%20TEE.pdf |
| _version_ | 1797011370710925312 |
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| author | Lim, Kah Tee |
| author_facet | Lim, Kah Tee |
| author_sort | Lim, Kah Tee |
| collection | USM |
| description | In order to accelerate the discovery of novel leads for the treatment of Human African Trypanosomiasis (HAT), it is necessary to have a simple and sensitive assay to identify positive hits by whole cell viability based high-throughput screening (HTS). In this study, the HTS assay was developed in 384-well format using clear plate and black plate, for comparison. Assay robustness and reproducibility were determined under the optimized conditions in 384-well plate was well tolerated in the HTS assay, including percentage of coefficient of variation of 4.68% and 4.74%, signal-to-background ratio of 12.75 and 12.07, and Z’ factor of 0.79 and 0.82 in clear- and black-384-well plate, respectively. |
| first_indexed | 2024-03-06T15:33:22Z |
| format | Thesis |
| id | usm.eprints-45404 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T15:33:22Z |
| publishDate | 2017 |
| record_format | dspace |
| spelling | usm.eprints-454042019-09-12T02:08:57Z http://eprints.usm.my/45404/ High-Throughput Screening Assay In 384-Well Format For The Identification Of Anti-Trypanosomal Agents Against Trypanosoma Brucei Rhodesiense And Mode Of Cell Death Study Lim, Kah Tee QH1-278.5 Natural history (General) In order to accelerate the discovery of novel leads for the treatment of Human African Trypanosomiasis (HAT), it is necessary to have a simple and sensitive assay to identify positive hits by whole cell viability based high-throughput screening (HTS). In this study, the HTS assay was developed in 384-well format using clear plate and black plate, for comparison. Assay robustness and reproducibility were determined under the optimized conditions in 384-well plate was well tolerated in the HTS assay, including percentage of coefficient of variation of 4.68% and 4.74%, signal-to-background ratio of 12.75 and 12.07, and Z’ factor of 0.79 and 0.82 in clear- and black-384-well plate, respectively. 2017-02 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/45404/1/LIM%20KAH%20TEE.pdf Lim, Kah Tee (2017) High-Throughput Screening Assay In 384-Well Format For The Identification Of Anti-Trypanosomal Agents Against Trypanosoma Brucei Rhodesiense And Mode Of Cell Death Study. PhD thesis, Universiti Sains Malaysia. |
| spellingShingle | QH1-278.5 Natural history (General) Lim, Kah Tee High-Throughput Screening Assay In 384-Well Format For The Identification Of Anti-Trypanosomal Agents Against Trypanosoma Brucei Rhodesiense And Mode Of Cell Death Study |
| title | High-Throughput Screening Assay In 384-Well Format For The Identification Of Anti-Trypanosomal Agents Against Trypanosoma Brucei Rhodesiense And Mode Of Cell Death Study |
| title_full | High-Throughput Screening Assay In 384-Well Format For The Identification Of Anti-Trypanosomal Agents Against Trypanosoma Brucei Rhodesiense And Mode Of Cell Death Study |
| title_fullStr | High-Throughput Screening Assay In 384-Well Format For The Identification Of Anti-Trypanosomal Agents Against Trypanosoma Brucei Rhodesiense And Mode Of Cell Death Study |
| title_full_unstemmed | High-Throughput Screening Assay In 384-Well Format For The Identification Of Anti-Trypanosomal Agents Against Trypanosoma Brucei Rhodesiense And Mode Of Cell Death Study |
| title_short | High-Throughput Screening Assay In 384-Well Format For The Identification Of Anti-Trypanosomal Agents Against Trypanosoma Brucei Rhodesiense And Mode Of Cell Death Study |
| title_sort | high throughput screening assay in 384 well format for the identification of anti trypanosomal agents against trypanosoma brucei rhodesiense and mode of cell death study |
| topic | QH1-278.5 Natural history (General) |
| url | http://eprints.usm.my/45404/1/LIM%20KAH%20TEE.pdf |
| work_keys_str_mv | AT limkahtee highthroughputscreeningassayin384wellformatfortheidentificationofantitrypanosomalagentsagainsttrypanosomabruceirhodesienseandmodeofcelldeathstudy |