Development Of A Novel T/A Cloning And Expression Vector For Efficient Expression Of Protein-Based Biopharmaceuticals In Escherichia Coli
Escherichia coli is one of the most popular bacterial hosts for handling, manipulating, and expressing target genes of interest. Certain genes are difficult to express, often due to scarcity of specific tRNA. This has led to little and/or poor quality protein production in E. coli. To alleviate this...
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| Format: | Thesis |
| Language: | English |
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2017
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| Online Access: | http://eprints.usm.my/45424/1/MUHAMAD%20ALIF%20CHE%20NORDIN.pdf |
| _version_ | 1825835150324269056 |
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| author | Che Nordin, Muhamad Alif |
| author_facet | Che Nordin, Muhamad Alif |
| author_sort | Che Nordin, Muhamad Alif |
| collection | USM |
| description | Escherichia coli is one of the most popular bacterial hosts for handling, manipulating, and expressing target genes of interest. Certain genes are difficult to express, often due to scarcity of specific tRNA. This has led to little and/or poor quality protein production in E. coli. To alleviate this problem, E. coli hosts are transformed with rare tRNA-expressing helper plasmid vectors. Maintenance of two different plasmid vectors-expressing the gene of interest and rare tRNA genes exert metabolic burden on the host that may result in diminished growth and reduced protein production. |
| first_indexed | 2024-03-06T15:33:25Z |
| format | Thesis |
| id | usm.eprints-45424 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T15:33:25Z |
| publishDate | 2017 |
| record_format | dspace |
| spelling | usm.eprints-454242019-09-17T01:59:16Z http://eprints.usm.my/45424/ Development Of A Novel T/A Cloning And Expression Vector For Efficient Expression Of Protein-Based Biopharmaceuticals In Escherichia Coli Che Nordin, Muhamad Alif R735-854 Medical education. Medical schools. Research Escherichia coli is one of the most popular bacterial hosts for handling, manipulating, and expressing target genes of interest. Certain genes are difficult to express, often due to scarcity of specific tRNA. This has led to little and/or poor quality protein production in E. coli. To alleviate this problem, E. coli hosts are transformed with rare tRNA-expressing helper plasmid vectors. Maintenance of two different plasmid vectors-expressing the gene of interest and rare tRNA genes exert metabolic burden on the host that may result in diminished growth and reduced protein production. 2017-05 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/45424/1/MUHAMAD%20ALIF%20CHE%20NORDIN.pdf Che Nordin, Muhamad Alif (2017) Development Of A Novel T/A Cloning And Expression Vector For Efficient Expression Of Protein-Based Biopharmaceuticals In Escherichia Coli. Masters thesis, Universiti Sains Malaysia. |
| spellingShingle | R735-854 Medical education. Medical schools. Research Che Nordin, Muhamad Alif Development Of A Novel T/A Cloning And Expression Vector For Efficient Expression Of Protein-Based Biopharmaceuticals In Escherichia Coli |
| title | Development Of A Novel T/A Cloning And Expression Vector For Efficient Expression Of Protein-Based Biopharmaceuticals In Escherichia Coli |
| title_full | Development Of A Novel T/A Cloning And Expression Vector For Efficient Expression Of Protein-Based Biopharmaceuticals In Escherichia Coli |
| title_fullStr | Development Of A Novel T/A Cloning And Expression Vector For Efficient Expression Of Protein-Based Biopharmaceuticals In Escherichia Coli |
| title_full_unstemmed | Development Of A Novel T/A Cloning And Expression Vector For Efficient Expression Of Protein-Based Biopharmaceuticals In Escherichia Coli |
| title_short | Development Of A Novel T/A Cloning And Expression Vector For Efficient Expression Of Protein-Based Biopharmaceuticals In Escherichia Coli |
| title_sort | development of a novel t a cloning and expression vector for efficient expression of protein based biopharmaceuticals in escherichia coli |
| topic | R735-854 Medical education. Medical schools. Research |
| url | http://eprints.usm.my/45424/1/MUHAMAD%20ALIF%20CHE%20NORDIN.pdf |
| work_keys_str_mv | AT chenordinmuhamadalif developmentofanoveltacloningandexpressionvectorforefficientexpressionofproteinbasedbiopharmaceuticalsinescherichiacoli |