Human molar dentinal tubules and its odontoblast process. A combined investigation using variable pressure scanning electron microscope and image analysis

Electron microscopy studies of the dentinal tubules are mainly investigated using the high vacuum conventional scanning electron microscope (SEM). We aimed to investigate its morphology using the variable pressure SEM Three almost caries free intact adult human molar tooth were prepared as unembe...

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Main Authors: Ghazali, Farid Che, Noor, Syed Hatim, Asan, Jamaruddin Mat
Format: Article
Language:English
Published: Pusat Pengajian Sains Perubatan, Universiti Sains Malaysia 2004
Subjects:
Online Access:http://eprints.usm.my/45609/1/GP...Human%20Molar%20Dentinal%20Tubles%20And%20Its%20Odontoblast%20Process...2004...-OCR.pdf
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author Ghazali, Farid Che
Noor, Syed Hatim
Asan, Jamaruddin Mat
author_facet Ghazali, Farid Che
Noor, Syed Hatim
Asan, Jamaruddin Mat
author_sort Ghazali, Farid Che
collection USM
description Electron microscopy studies of the dentinal tubules are mainly investigated using the high vacuum conventional scanning electron microscope (SEM). We aimed to investigate its morphology using the variable pressure SEM Three almost caries free intact adult human molar tooth were prepared as unembedded demineralised whole dentine preparations that were initially chemically fixed with 1 Oo/o formalin for 24 hours. They were demineralised in 1 0°/o EDTA solution for 4 weeks following, which they were than section into two equal halves. The uncoated partially demineralised dentine half tissue was inspected systematically under a LEO VPSEM using the peltier cooling sub stage at 15 Pascal pressure with an accelerating voltage of 15 kV. An occlusal and longitudinal observation was made from its occlusal surface to the pulpal border. Both low and high magnifications images were prepared from each specimen observed.
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spelling usm.eprints-456092019-10-10T06:56:21Z http://eprints.usm.my/45609/ Human molar dentinal tubules and its odontoblast process. A combined investigation using variable pressure scanning electron microscope and image analysis Ghazali, Farid Che Noor, Syed Hatim Asan, Jamaruddin Mat R Medicine Electron microscopy studies of the dentinal tubules are mainly investigated using the high vacuum conventional scanning electron microscope (SEM). We aimed to investigate its morphology using the variable pressure SEM Three almost caries free intact adult human molar tooth were prepared as unembedded demineralised whole dentine preparations that were initially chemically fixed with 1 Oo/o formalin for 24 hours. They were demineralised in 1 0°/o EDTA solution for 4 weeks following, which they were than section into two equal halves. The uncoated partially demineralised dentine half tissue was inspected systematically under a LEO VPSEM using the peltier cooling sub stage at 15 Pascal pressure with an accelerating voltage of 15 kV. An occlusal and longitudinal observation was made from its occlusal surface to the pulpal border. Both low and high magnifications images were prepared from each specimen observed. Pusat Pengajian Sains Perubatan, Universiti Sains Malaysia 2004 Article NonPeerReviewed application/pdf en http://eprints.usm.my/45609/1/GP...Human%20Molar%20Dentinal%20Tubles%20And%20Its%20Odontoblast%20Process...2004...-OCR.pdf Ghazali, Farid Che and Noor, Syed Hatim and Asan, Jamaruddin Mat (2004) Human molar dentinal tubules and its odontoblast process. A combined investigation using variable pressure scanning electron microscope and image analysis. Human molar dentinal tubules and its odontoblast process. A combined investigation using variable pressure scanning electron microscope and image analysis.. (Submitted)
spellingShingle R Medicine
Ghazali, Farid Che
Noor, Syed Hatim
Asan, Jamaruddin Mat
Human molar dentinal tubules and its odontoblast process. A combined investigation using variable pressure scanning electron microscope and image analysis
title Human molar dentinal tubules and its odontoblast process. A combined investigation using variable pressure scanning electron microscope and image analysis
title_full Human molar dentinal tubules and its odontoblast process. A combined investigation using variable pressure scanning electron microscope and image analysis
title_fullStr Human molar dentinal tubules and its odontoblast process. A combined investigation using variable pressure scanning electron microscope and image analysis
title_full_unstemmed Human molar dentinal tubules and its odontoblast process. A combined investigation using variable pressure scanning electron microscope and image analysis
title_short Human molar dentinal tubules and its odontoblast process. A combined investigation using variable pressure scanning electron microscope and image analysis
title_sort human molar dentinal tubules and its odontoblast process a combined investigation using variable pressure scanning electron microscope and image analysis
topic R Medicine
url http://eprints.usm.my/45609/1/GP...Human%20Molar%20Dentinal%20Tubles%20And%20Its%20Odontoblast%20Process...2004...-OCR.pdf
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AT noorsyedhatim humanmolardentinaltubulesanditsodontoblastprocessacombinedinvestigationusingvariablepressurescanningelectronmicroscopeandimageanalysis
AT asanjamaruddinmat humanmolardentinaltubulesanditsodontoblastprocessacombinedinvestigationusingvariablepressurescanningelectronmicroscopeandimageanalysis