Kallikrein-kinin system in human fetoplacental tissues in pregnancy -induced hypertension
The pathogenesis·~ of pregnancy-induced ···hypertension·· (Pil-l) remains an enigma. However, it is currently postulated that placental hypoperfusion due to an imbalance of vasoconstrictor and vasodilator substances in the placenta may contribute to the pathogenesis of PIH. Hence, the objectives...
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| Format: | Article |
| Language: | English |
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Pusat Pengajian Sains Perubatan, Universiti Sains Malaysia
2004
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| Online Access: | http://eprints.usm.my/45613/1/GP...Kallikrein-Kinin%20System%20In%20Human%20Fetoplacental%20Tissues%20In%20Pregnancy-Induced%20Hypertension...2004...-24%20pages.pdf |
| _version_ | 1797011411059081216 |
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| author | Larmie, E.T. Singh, Harbindar Jeet Shukri, Mohd |
| author_facet | Larmie, E.T. Singh, Harbindar Jeet Shukri, Mohd |
| author_sort | Larmie, E.T. |
| collection | USM |
| description | The pathogenesis·~ of pregnancy-induced ···hypertension·· (Pil-l) remains an
enigma. However, it is currently postulated that placental hypoperfusion due
to an imbalance of vasoconstrictor and vasodilator substances in the placenta
may contribute to the pathogenesis of PIH. Hence, the objectives of this study
are: (1) to determine the kininogen {using enzyme immunoassay) and tissue
kallikrein levels (using a synthetic chromogenic substrate, S-2266), the
substrate and enzyme, respectively, that are responsible for the generation of
kinin (a vasodilator); (2) to determine the molecular weight of tissue kallikrein
(by Western blot analysis); and (3) to determine the gross effect of
fetoplacental tissue extracts on the contraction of rabbit jejunum smooth
muscle using various fetoplacental tissues from 12 normotensive pregnant
women and 12 women with PIH. No significant differences were found for
active, total and inactive tissue kallikrein levels in tissues from both groups.
However, kininogen levels and kininogen/total tissue kallikrein ratios were
significantly lower in chorion laeve, placental plate chorion, fetal placenta and
maternal placenta from women with PIH. The molecular weight of tissue
kallikrein was about 52 kDa for both groups. These findings might indicate a
lower level of kinin generation in fetoplacental tissues of women with PIH
confirming the reported preponderance of vasoconstrictor activity. The higher
increase in smooth muscle tone produced by application of the fetoplacental
tissue extracts from women with PIH also further supports this postulate. |
| first_indexed | 2024-03-06T15:33:58Z |
| format | Article |
| id | usm.eprints-45613 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T15:33:58Z |
| publishDate | 2004 |
| publisher | Pusat Pengajian Sains Perubatan, Universiti Sains Malaysia |
| record_format | dspace |
| spelling | usm.eprints-456132019-10-10T08:34:26Z http://eprints.usm.my/45613/ Kallikrein-kinin system in human fetoplacental tissues in pregnancy -induced hypertension Larmie, E.T. Singh, Harbindar Jeet Shukri, Mohd RG Gynecology and obstetrics The pathogenesis·~ of pregnancy-induced ···hypertension·· (Pil-l) remains an enigma. However, it is currently postulated that placental hypoperfusion due to an imbalance of vasoconstrictor and vasodilator substances in the placenta may contribute to the pathogenesis of PIH. Hence, the objectives of this study are: (1) to determine the kininogen {using enzyme immunoassay) and tissue kallikrein levels (using a synthetic chromogenic substrate, S-2266), the substrate and enzyme, respectively, that are responsible for the generation of kinin (a vasodilator); (2) to determine the molecular weight of tissue kallikrein (by Western blot analysis); and (3) to determine the gross effect of fetoplacental tissue extracts on the contraction of rabbit jejunum smooth muscle using various fetoplacental tissues from 12 normotensive pregnant women and 12 women with PIH. No significant differences were found for active, total and inactive tissue kallikrein levels in tissues from both groups. However, kininogen levels and kininogen/total tissue kallikrein ratios were significantly lower in chorion laeve, placental plate chorion, fetal placenta and maternal placenta from women with PIH. The molecular weight of tissue kallikrein was about 52 kDa for both groups. These findings might indicate a lower level of kinin generation in fetoplacental tissues of women with PIH confirming the reported preponderance of vasoconstrictor activity. The higher increase in smooth muscle tone produced by application of the fetoplacental tissue extracts from women with PIH also further supports this postulate. Pusat Pengajian Sains Perubatan, Universiti Sains Malaysia 2004 Article NonPeerReviewed application/pdf en http://eprints.usm.my/45613/1/GP...Kallikrein-Kinin%20System%20In%20Human%20Fetoplacental%20Tissues%20In%20Pregnancy-Induced%20Hypertension...2004...-24%20pages.pdf Larmie, E.T. and Singh, Harbindar Jeet and Shukri, Mohd (2004) Kallikrein-kinin system in human fetoplacental tissues in pregnancy -induced hypertension. Kallikrein-kinin system in human fetoplacental tissues in pregnancy -induced hypertension. (Submitted) |
| spellingShingle | RG Gynecology and obstetrics Larmie, E.T. Singh, Harbindar Jeet Shukri, Mohd Kallikrein-kinin system in human fetoplacental tissues in pregnancy -induced hypertension |
| title | Kallikrein-kinin system in human
fetoplacental tissues in
pregnancy -induced
hypertension |
| title_full | Kallikrein-kinin system in human
fetoplacental tissues in
pregnancy -induced
hypertension |
| title_fullStr | Kallikrein-kinin system in human
fetoplacental tissues in
pregnancy -induced
hypertension |
| title_full_unstemmed | Kallikrein-kinin system in human
fetoplacental tissues in
pregnancy -induced
hypertension |
| title_short | Kallikrein-kinin system in human
fetoplacental tissues in
pregnancy -induced
hypertension |
| title_sort | kallikrein kinin system in human fetoplacental tissues in pregnancy induced hypertension |
| topic | RG Gynecology and obstetrics |
| url | http://eprints.usm.my/45613/1/GP...Kallikrein-Kinin%20System%20In%20Human%20Fetoplacental%20Tissues%20In%20Pregnancy-Induced%20Hypertension...2004...-24%20pages.pdf |
| work_keys_str_mv | AT larmieet kallikreinkininsysteminhumanfetoplacentaltissuesinpregnancyinducedhypertension AT singhharbindarjeet kallikreinkininsysteminhumanfetoplacentaltissuesinpregnancyinducedhypertension AT shukrimohd kallikreinkininsysteminhumanfetoplacentaltissuesinpregnancyinducedhypertension |