Biosynthesis And Characterization Of Poly(3-Hydroxybutyrate-Co-4-Hydroxybutyrate) Copolymer From Cupriavidus Sp. Usmahm13
Production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] using combination of waste glycerine and carbon precursor by bacteria is still very limited. Therefore, this research was conducted to (i) isolate and identify a bacterium that able to produce high PHA content and 4HB monomer...
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| Format: | Thesis |
| Language: | English |
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2013
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| Online Access: | http://eprints.usm.my/46279/1/Hema%20Ramachandran24.pdf |
| _version_ | 1825905530474856448 |
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| author | Ramachandran, Hema |
| author_facet | Ramachandran, Hema |
| author_sort | Ramachandran, Hema |
| collection | USM |
| description | Production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] using combination of waste glycerine and carbon precursor by bacteria is still very limited. Therefore, this research was conducted to (i) isolate and identify a bacterium that able to produce high PHA content and 4HB monomer composition, (ii) explore the ability of the bacterium to produce P(3HB-co-4HB) using various inexpensive and renewable carbon sources, (iii) optimize the P(3HB-co-4HB) copolymer production using glycerine pitch through shake-flask fermentation using response surface methodology (RSM) and (iv) evaluate the material characteristics of the P(3HB-co-4HB) copolymers with various 4HB monomer compositions. In this study, a novel yellow-pigmented bacterium which exhibited ability of producing P(3HB-co-4HB) copolymer was successfully isolated from Perak, Malaysia and designated as Cupriavidus sp. USMAHM13. Based on the phenotypic and genotypic analyses, it could be suggested that Cupriavidus sp. USMAHM13 represents a novel species within the genus Cupriavidus. Preliminary screening of carbon sources for biosynthesis of P(3HB-co-4HB) copolymer by Cupriavidus sp. USMAHM13 revealed that high 4HB monomer composition (43 mol%) with cell dry weight and PHA content of 6.0 g/l and 49 wt%, respectively was achieved through combination of glycerine pitch (5 g/l) and 1,4-butanediol (5 g/l) via one-stage cultivation. |
| first_indexed | 2024-03-06T15:35:51Z |
| format | Thesis |
| id | usm.eprints-46279 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T15:35:51Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | usm.eprints-462792020-02-20T02:15:30Z http://eprints.usm.my/46279/ Biosynthesis And Characterization Of Poly(3-Hydroxybutyrate-Co-4-Hydroxybutyrate) Copolymer From Cupriavidus Sp. Usmahm13 Ramachandran, Hema QH1-278.5 Natural history (General) Production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] using combination of waste glycerine and carbon precursor by bacteria is still very limited. Therefore, this research was conducted to (i) isolate and identify a bacterium that able to produce high PHA content and 4HB monomer composition, (ii) explore the ability of the bacterium to produce P(3HB-co-4HB) using various inexpensive and renewable carbon sources, (iii) optimize the P(3HB-co-4HB) copolymer production using glycerine pitch through shake-flask fermentation using response surface methodology (RSM) and (iv) evaluate the material characteristics of the P(3HB-co-4HB) copolymers with various 4HB monomer compositions. In this study, a novel yellow-pigmented bacterium which exhibited ability of producing P(3HB-co-4HB) copolymer was successfully isolated from Perak, Malaysia and designated as Cupriavidus sp. USMAHM13. Based on the phenotypic and genotypic analyses, it could be suggested that Cupriavidus sp. USMAHM13 represents a novel species within the genus Cupriavidus. Preliminary screening of carbon sources for biosynthesis of P(3HB-co-4HB) copolymer by Cupriavidus sp. USMAHM13 revealed that high 4HB monomer composition (43 mol%) with cell dry weight and PHA content of 6.0 g/l and 49 wt%, respectively was achieved through combination of glycerine pitch (5 g/l) and 1,4-butanediol (5 g/l) via one-stage cultivation. 2013-11 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/46279/1/Hema%20Ramachandran24.pdf Ramachandran, Hema (2013) Biosynthesis And Characterization Of Poly(3-Hydroxybutyrate-Co-4-Hydroxybutyrate) Copolymer From Cupriavidus Sp. Usmahm13. PhD thesis, Universiti Sains Malaysia. |
| spellingShingle | QH1-278.5 Natural history (General) Ramachandran, Hema Biosynthesis And Characterization Of Poly(3-Hydroxybutyrate-Co-4-Hydroxybutyrate) Copolymer From Cupriavidus Sp. Usmahm13 |
| title | Biosynthesis And Characterization Of Poly(3-Hydroxybutyrate-Co-4-Hydroxybutyrate) Copolymer From Cupriavidus Sp. Usmahm13 |
| title_full | Biosynthesis And Characterization Of Poly(3-Hydroxybutyrate-Co-4-Hydroxybutyrate) Copolymer From Cupriavidus Sp. Usmahm13 |
| title_fullStr | Biosynthesis And Characterization Of Poly(3-Hydroxybutyrate-Co-4-Hydroxybutyrate) Copolymer From Cupriavidus Sp. Usmahm13 |
| title_full_unstemmed | Biosynthesis And Characterization Of Poly(3-Hydroxybutyrate-Co-4-Hydroxybutyrate) Copolymer From Cupriavidus Sp. Usmahm13 |
| title_short | Biosynthesis And Characterization Of Poly(3-Hydroxybutyrate-Co-4-Hydroxybutyrate) Copolymer From Cupriavidus Sp. Usmahm13 |
| title_sort | biosynthesis and characterization of poly 3 hydroxybutyrate co 4 hydroxybutyrate copolymer from cupriavidus sp usmahm13 |
| topic | QH1-278.5 Natural history (General) |
| url | http://eprints.usm.my/46279/1/Hema%20Ramachandran24.pdf |
| work_keys_str_mv | AT ramachandranhema biosynthesisandcharacterizationofpoly3hydroxybutyrateco4hydroxybutyratecopolymerfromcupriavidusspusmahm13 |