Phytochemical Studies On Hylocereus Polyrhizus (Fruit And Stem) And Hylocereus Undatus (Fruit)

The volatile constituents for the fruits of Hylocereus polyrhizus and Hylocereus undatus were isolated by vacuum distillation and analysed by capillary GC and GC-MS, using two columns of different polarities. Fifty-seven and 58 components were identified in each of the fruits, representing 99.2% and...

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主要作者: Foong, Shin Yee
格式: Thesis
语言:English
出版: 2014
主题:
在线阅读:http://eprints.usm.my/46425/1/Foong%20Shin%20Yee24.pdf
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author Foong, Shin Yee
author_facet Foong, Shin Yee
author_sort Foong, Shin Yee
collection USM
description The volatile constituents for the fruits of Hylocereus polyrhizus and Hylocereus undatus were isolated by vacuum distillation and analysed by capillary GC and GC-MS, using two columns of different polarities. Fifty-seven and 58 components were identified in each of the fruits, representing 99.2% and 99.5% of the isolates, respectively. The volatile profiles of both fruits were quite similar, both of which being dominated by alcohols (H. polyrhizus, 36.5%; H. undatus, 38.6%) and carboxylic acids (H. polyrhizus, 35.1%; H. undatus, 22.3%), with 1-hexanol and squalene as the two major components. The volatile constituents of H. polyrhizus showed stronger antibacterial activity than those of H. undatus when tested against five bacteria (Bacillus subtilis subsp. spizizenii, Escherichia coli, Klebsiella pneumonia, Pseudomonas stutzeri and Staphylococcus aureus) using the micro-dilution antibacterial assay. This is the first report for the volatile constituents and antibacterial acitivities of the both Hylocereus species. The phytochemical investigation on H. polyrhizus had led to the isolation and identification of six compounds. Myo-inositol (C1) was isolated from the methanolic fruit extract while octatriacont-1-ene (C2), lupeol (C3), stigmasterol (C4), a mixture of stigmasterol and β-sitosterol (C5) and quercetin (C6) were isolated from the chloroform extract of the stems. The structures of these compounds were elucidated by spectroscopic techniques such as IR, DP-MS, 1D-NMR, 2D-NMR and UV-Vis.
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spelling usm.eprints-464252020-03-04T06:28:54Z http://eprints.usm.my/46425/ Phytochemical Studies On Hylocereus Polyrhizus (Fruit And Stem) And Hylocereus Undatus (Fruit) Foong, Shin Yee TP1-1185 Chemical technology The volatile constituents for the fruits of Hylocereus polyrhizus and Hylocereus undatus were isolated by vacuum distillation and analysed by capillary GC and GC-MS, using two columns of different polarities. Fifty-seven and 58 components were identified in each of the fruits, representing 99.2% and 99.5% of the isolates, respectively. The volatile profiles of both fruits were quite similar, both of which being dominated by alcohols (H. polyrhizus, 36.5%; H. undatus, 38.6%) and carboxylic acids (H. polyrhizus, 35.1%; H. undatus, 22.3%), with 1-hexanol and squalene as the two major components. The volatile constituents of H. polyrhizus showed stronger antibacterial activity than those of H. undatus when tested against five bacteria (Bacillus subtilis subsp. spizizenii, Escherichia coli, Klebsiella pneumonia, Pseudomonas stutzeri and Staphylococcus aureus) using the micro-dilution antibacterial assay. This is the first report for the volatile constituents and antibacterial acitivities of the both Hylocereus species. The phytochemical investigation on H. polyrhizus had led to the isolation and identification of six compounds. Myo-inositol (C1) was isolated from the methanolic fruit extract while octatriacont-1-ene (C2), lupeol (C3), stigmasterol (C4), a mixture of stigmasterol and β-sitosterol (C5) and quercetin (C6) were isolated from the chloroform extract of the stems. The structures of these compounds were elucidated by spectroscopic techniques such as IR, DP-MS, 1D-NMR, 2D-NMR and UV-Vis. 2014-02 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/46425/1/Foong%20Shin%20Yee24.pdf Foong, Shin Yee (2014) Phytochemical Studies On Hylocereus Polyrhizus (Fruit And Stem) And Hylocereus Undatus (Fruit). Masters thesis, Universiti Sains Malaysia.
spellingShingle TP1-1185 Chemical technology
Foong, Shin Yee
Phytochemical Studies On Hylocereus Polyrhizus (Fruit And Stem) And Hylocereus Undatus (Fruit)
title Phytochemical Studies On Hylocereus Polyrhizus (Fruit And Stem) And Hylocereus Undatus (Fruit)
title_full Phytochemical Studies On Hylocereus Polyrhizus (Fruit And Stem) And Hylocereus Undatus (Fruit)
title_fullStr Phytochemical Studies On Hylocereus Polyrhizus (Fruit And Stem) And Hylocereus Undatus (Fruit)
title_full_unstemmed Phytochemical Studies On Hylocereus Polyrhizus (Fruit And Stem) And Hylocereus Undatus (Fruit)
title_short Phytochemical Studies On Hylocereus Polyrhizus (Fruit And Stem) And Hylocereus Undatus (Fruit)
title_sort phytochemical studies on hylocereus polyrhizus fruit and stem and hylocereus undatus fruit
topic TP1-1185 Chemical technology
url http://eprints.usm.my/46425/1/Foong%20Shin%20Yee24.pdf
work_keys_str_mv AT foongshinyee phytochemicalstudiesonhylocereuspolyrhizusfruitandstemandhylocereusundatusfruit