Xanthorrhizol Induces Apoptosis Via the Up-regulation of Bax and p53 in HeLa Cells
Xanthorrhizol is a sesquiterpenoid compound extracted from Curcuma xanthorrhiza, which is known locally as Temulawak. Traditionally, C. xanthorrhiza was found to have antibacterial, anticancer and anti-inflammatory activity. The rhizome has also been used to treat inflammation in postpartum ute...
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| Format: | Article |
| Language: | English |
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International Institute of Anticancer Research (IIAR)
2005
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| Subjects: | |
| Online Access: | http://eprints.usm.my/46484/1/paperanticancerresearch.pdf |
| _version_ | 1825905570509488128 |
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| author | Ismail, Norzila Pihie, Azimahtol Hawariah Lope Nallapan, Meenakshii |
| author_facet | Ismail, Norzila Pihie, Azimahtol Hawariah Lope Nallapan, Meenakshii |
| author_sort | Ismail, Norzila |
| collection | USM |
| description | Xanthorrhizol is a sesquiterpenoid compound
extracted from Curcuma xanthorrhiza, which is known locally
as Temulawak. Traditionally, C. xanthorrhiza was found to
have antibacterial, anticancer and anti-inflammatory activity.
The rhizome has also been used to treat inflammation in
postpartum uterine bleeding. An antiproliferative assay using
methylene blue staining revealed that xanthorrhizol inhibited
the proliferation of the cervical cancer cell line HeLa with an
EC50 value of 6.16 Ìg/ml. Xanthorrhizol significantly increased
apoptosis in HeLa cells, as evaluated by the Tdt-mediated
dUTP nick end-labelling (TUNEL) assay and nuclear
morphology by Hoechst 33258 staining. Western blot analysis,
which was further confirmed by the immunostaining results,
implied an up-regulation of tumor suppressor protein p53 and
the pro-apoptotic protein Bax, following the treatment with
xanthorrhizol. Xanthorrhizol, however, did not affect the
expression of the anti-apoptotic protein, Bcl-2 and the viral
oncoprotein, E6. Hence, xanthorrhizol is a promising
antiproliferative and anticancer agent which induces p53 and
Bax-dependent apoptosis in HeLa cervical cancer cells |
| first_indexed | 2024-03-06T15:36:26Z |
| format | Article |
| id | usm.eprints-46484 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T15:36:26Z |
| publishDate | 2005 |
| publisher | International Institute of Anticancer Research (IIAR) |
| record_format | dspace |
| spelling | usm.eprints-464842020-03-11T07:37:02Z http://eprints.usm.my/46484/ Xanthorrhizol Induces Apoptosis Via the Up-regulation of Bax and p53 in HeLa Cells Ismail, Norzila Pihie, Azimahtol Hawariah Lope Nallapan, Meenakshii RC254-282 Neoplasms. Tumors. Oncology (including Cancer) Xanthorrhizol is a sesquiterpenoid compound extracted from Curcuma xanthorrhiza, which is known locally as Temulawak. Traditionally, C. xanthorrhiza was found to have antibacterial, anticancer and anti-inflammatory activity. The rhizome has also been used to treat inflammation in postpartum uterine bleeding. An antiproliferative assay using methylene blue staining revealed that xanthorrhizol inhibited the proliferation of the cervical cancer cell line HeLa with an EC50 value of 6.16 Ìg/ml. Xanthorrhizol significantly increased apoptosis in HeLa cells, as evaluated by the Tdt-mediated dUTP nick end-labelling (TUNEL) assay and nuclear morphology by Hoechst 33258 staining. Western blot analysis, which was further confirmed by the immunostaining results, implied an up-regulation of tumor suppressor protein p53 and the pro-apoptotic protein Bax, following the treatment with xanthorrhizol. Xanthorrhizol, however, did not affect the expression of the anti-apoptotic protein, Bcl-2 and the viral oncoprotein, E6. Hence, xanthorrhizol is a promising antiproliferative and anticancer agent which induces p53 and Bax-dependent apoptosis in HeLa cervical cancer cells International Institute of Anticancer Research (IIAR) 2005 Article PeerReviewed application/pdf en http://eprints.usm.my/46484/1/paperanticancerresearch.pdf Ismail, Norzila and Pihie, Azimahtol Hawariah Lope and Nallapan, Meenakshii (2005) Xanthorrhizol Induces Apoptosis Via the Up-regulation of Bax and p53 in HeLa Cells. Anticancer Research, 25 (3B). pp. 2221-2227. ISSN 0250-7005 http://ar.iiarjournals.org/content/25/3B/2221.short |
| spellingShingle | RC254-282 Neoplasms. Tumors. Oncology (including Cancer) Ismail, Norzila Pihie, Azimahtol Hawariah Lope Nallapan, Meenakshii Xanthorrhizol Induces Apoptosis Via the Up-regulation of Bax and p53 in HeLa Cells |
| title | Xanthorrhizol Induces Apoptosis Via the
Up-regulation of Bax and p53 in HeLa Cells |
| title_full | Xanthorrhizol Induces Apoptosis Via the
Up-regulation of Bax and p53 in HeLa Cells |
| title_fullStr | Xanthorrhizol Induces Apoptosis Via the
Up-regulation of Bax and p53 in HeLa Cells |
| title_full_unstemmed | Xanthorrhizol Induces Apoptosis Via the
Up-regulation of Bax and p53 in HeLa Cells |
| title_short | Xanthorrhizol Induces Apoptosis Via the
Up-regulation of Bax and p53 in HeLa Cells |
| title_sort | xanthorrhizol induces apoptosis via the up regulation of bax and p53 in hela cells |
| topic | RC254-282 Neoplasms. Tumors. Oncology (including Cancer) |
| url | http://eprints.usm.my/46484/1/paperanticancerresearch.pdf |
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