Phytocompounds of Anonna muricata leaves extract and cytotoxic effects on breast cancer cells
Objective: To identify the phytochemical compounds from Annona muricata (A. muricata) and to determine their in vitro anti-proliferative activities against breast cancer cells, MCF7 and MDA-MB-231. Methods: A. muricata leaves were successively extracted by soxhlet method using n-hexane, ethyl ace...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Hainan Medical University, Institute of Tropical Disease
2018
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| Subjects: | |
| Online Access: | http://eprints.usm.my/46485/1/AsianPacJTropMed1112659-2888206_080122.pdf |
| _version_ | 1797011583644205056 |
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| author | Rahman, Husna Syakirah Ab Ibrahim, Wan Suriyani Wan Ismail, Norzila Ismail, Tuan Nadrah Naim Tuan Salleh, Siti Farhanah Mohd Pak, Michael Kai Wong Samad, Mohd Ridzuan Abdul Hashim, Mohd Nizam Md |
| author_facet | Rahman, Husna Syakirah Ab Ibrahim, Wan Suriyani Wan Ismail, Norzila Ismail, Tuan Nadrah Naim Tuan Salleh, Siti Farhanah Mohd Pak, Michael Kai Wong Samad, Mohd Ridzuan Abdul Hashim, Mohd Nizam Md |
| author_sort | Rahman, Husna Syakirah Ab |
| collection | USM |
| description | Objective: To identify the phytochemical compounds from Annona muricata (A. muricata)
and to determine their in vitro anti-proliferative activities against breast cancer cells, MCF7
and MDA-MB-231. Methods: A. muricata leaves were successively extracted by soxhlet
method using n-hexane, ethyl acetate and methanol, and decocted with water. Each extract was
analysed by gas chromatography mass spectrometry (GCMS) and characterized with Wiley
and NIST library searches. Anti-proliferative activity of each extract was evaluated on MCF7
and MDA-MB-231 breast cancer cells using MTT assay. Results: The GCMS analysis of
different solvent extracts of A. muricata leaves showed presence of different chemical groups
of compounds such as steroids, terpenoids, phenolic compounds, sugars, sugars alcohol and
others including vitamin E. Ethyl acetate leaves extract exhibited the lowest IC50 value on the
MDA-MB-231 breast cancer cell and n-hexane leaves extract showed the the lowest IC50 value
on the MCF-7 breast cancer cell. Conclusion: Steroids and phenolic compounds were the main
phytocompound groups identified from all A. muricata leaves extracts. The antiproliferative
activity of n-hexane and ethyl acetate extract towards breast cancer MCF7 and MDA-MB-231
respectively might be due to the presence of biologically active compounds in the extracts,
hence, providing some scientific evidences of the effectiveness of its traditional usages. |
| first_indexed | 2024-03-06T15:36:26Z |
| format | Article |
| id | usm.eprints-46485 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T15:36:26Z |
| publishDate | 2018 |
| publisher | Hainan Medical University, Institute of Tropical Disease |
| record_format | dspace |
| spelling | usm.eprints-464852020-03-11T08:16:31Z http://eprints.usm.my/46485/ Phytocompounds of Anonna muricata leaves extract and cytotoxic effects on breast cancer cells Rahman, Husna Syakirah Ab Ibrahim, Wan Suriyani Wan Ismail, Norzila Ismail, Tuan Nadrah Naim Tuan Salleh, Siti Farhanah Mohd Pak, Michael Kai Wong Samad, Mohd Ridzuan Abdul Hashim, Mohd Nizam Md RC254-282 Neoplasms. Tumors. Oncology (including Cancer) Objective: To identify the phytochemical compounds from Annona muricata (A. muricata) and to determine their in vitro anti-proliferative activities against breast cancer cells, MCF7 and MDA-MB-231. Methods: A. muricata leaves were successively extracted by soxhlet method using n-hexane, ethyl acetate and methanol, and decocted with water. Each extract was analysed by gas chromatography mass spectrometry (GCMS) and characterized with Wiley and NIST library searches. Anti-proliferative activity of each extract was evaluated on MCF7 and MDA-MB-231 breast cancer cells using MTT assay. Results: The GCMS analysis of different solvent extracts of A. muricata leaves showed presence of different chemical groups of compounds such as steroids, terpenoids, phenolic compounds, sugars, sugars alcohol and others including vitamin E. Ethyl acetate leaves extract exhibited the lowest IC50 value on the MDA-MB-231 breast cancer cell and n-hexane leaves extract showed the the lowest IC50 value on the MCF-7 breast cancer cell. Conclusion: Steroids and phenolic compounds were the main phytocompound groups identified from all A. muricata leaves extracts. The antiproliferative activity of n-hexane and ethyl acetate extract towards breast cancer MCF7 and MDA-MB-231 respectively might be due to the presence of biologically active compounds in the extracts, hence, providing some scientific evidences of the effectiveness of its traditional usages. Hainan Medical University, Institute of Tropical Disease 2018 Article PeerReviewed application/pdf en http://eprints.usm.my/46485/1/AsianPacJTropMed1112659-2888206_080122.pdf Rahman, Husna Syakirah Ab and Ibrahim, Wan Suriyani Wan and Ismail, Norzila and Ismail, Tuan Nadrah Naim Tuan and Salleh, Siti Farhanah Mohd and Pak, Michael Kai Wong and Samad, Mohd Ridzuan Abdul and Hashim, Mohd Nizam Md (2018) Phytocompounds of Anonna muricata leaves extract and cytotoxic effects on breast cancer cells. Asian Pacific Journal of Tropical Medicine, 11 (12). pp. 659-665. ISSN 1995-7645 http://www.apjtm.org/article.asp?issn=1995-7645;year=2018;volume=11;issue=12;spage=659;epage=665;aulast=Rahman |
| spellingShingle | RC254-282 Neoplasms. Tumors. Oncology (including Cancer) Rahman, Husna Syakirah Ab Ibrahim, Wan Suriyani Wan Ismail, Norzila Ismail, Tuan Nadrah Naim Tuan Salleh, Siti Farhanah Mohd Pak, Michael Kai Wong Samad, Mohd Ridzuan Abdul Hashim, Mohd Nizam Md Phytocompounds of Anonna muricata leaves extract and cytotoxic effects on breast cancer cells |
| title | Phytocompounds of Anonna muricata leaves extract and cytotoxic effects
on breast cancer cells |
| title_full | Phytocompounds of Anonna muricata leaves extract and cytotoxic effects
on breast cancer cells |
| title_fullStr | Phytocompounds of Anonna muricata leaves extract and cytotoxic effects
on breast cancer cells |
| title_full_unstemmed | Phytocompounds of Anonna muricata leaves extract and cytotoxic effects
on breast cancer cells |
| title_short | Phytocompounds of Anonna muricata leaves extract and cytotoxic effects
on breast cancer cells |
| title_sort | phytocompounds of anonna muricata leaves extract and cytotoxic effects on breast cancer cells |
| topic | RC254-282 Neoplasms. Tumors. Oncology (including Cancer) |
| url | http://eprints.usm.my/46485/1/AsianPacJTropMed1112659-2888206_080122.pdf |
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