Microbial Production Of Polyhydroxybutyrate (Phb) By Burkholderia Cepacia Bpt1213 From Different Carbon Sources And Its Fermentation Kinetics

The issue of plastic pollution has become a major threat to global ecology due to plastic’s resilience against degradation and its proliferation in industry. As a result, the biodegradable polymer such as polyhydroxyalkanoate (PHA) is considered as a good substitute to the conventional petroleum-...

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Main Author: Wen Chian, Liew
Format: Monograph
Language:English
Published: Pusat Pengajian Teknologi Industri 2020
Subjects:
Online Access:http://eprints.usm.my/46844/1/MICROBIAL%20PRODUCTION%20OF%20%28ABSTRACT%29.pdf
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author Wen Chian, Liew
author_facet Wen Chian, Liew
author_sort Wen Chian, Liew
collection USM
description The issue of plastic pollution has become a major threat to global ecology due to plastic’s resilience against degradation and its proliferation in industry. As a result, the biodegradable polymer such as polyhydroxyalkanoate (PHA) is considered as a good substitute to the conventional petroleum-based polymers because of their similar material properties to synthetic polymers and complete biodegradability after disposal. However, PHA homopolymer, poly-3-hydroxybutyrate (P(3HB)) is the most common and widely studied PHA compounds. In this study, the biosynthesis of P(3HB) polymer produced by Burkholderia cepacia BPT1213 using different carbon source such as banana trunk juices, molasses and oil palm frond juices was studied. Besides, the sugar contents (sucrose, glucose and fructose) of these carbon sources were analysed. Molasses has the highest sucrose, glucose and fructose content (8.41 ± 0.50 mg/mL, 35.34 ± 0.01 mg/mL and 33.88 ± 0.06 mg/mL respectively). Next, kinetic study of B. cepacia BPT1213 such as specific growth rate, doubling time, biomass and PHA productivity were investigated for greater understanding of the fermentation efficiency.
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spelling usm.eprints-468442020-08-27T02:11:17Z http://eprints.usm.my/46844/ Microbial Production Of Polyhydroxybutyrate (Phb) By Burkholderia Cepacia Bpt1213 From Different Carbon Sources And Its Fermentation Kinetics Wen Chian, Liew T1-995 Technology(General) The issue of plastic pollution has become a major threat to global ecology due to plastic’s resilience against degradation and its proliferation in industry. As a result, the biodegradable polymer such as polyhydroxyalkanoate (PHA) is considered as a good substitute to the conventional petroleum-based polymers because of their similar material properties to synthetic polymers and complete biodegradability after disposal. However, PHA homopolymer, poly-3-hydroxybutyrate (P(3HB)) is the most common and widely studied PHA compounds. In this study, the biosynthesis of P(3HB) polymer produced by Burkholderia cepacia BPT1213 using different carbon source such as banana trunk juices, molasses and oil palm frond juices was studied. Besides, the sugar contents (sucrose, glucose and fructose) of these carbon sources were analysed. Molasses has the highest sucrose, glucose and fructose content (8.41 ± 0.50 mg/mL, 35.34 ± 0.01 mg/mL and 33.88 ± 0.06 mg/mL respectively). Next, kinetic study of B. cepacia BPT1213 such as specific growth rate, doubling time, biomass and PHA productivity were investigated for greater understanding of the fermentation efficiency. Pusat Pengajian Teknologi Industri 2020-07 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/46844/1/MICROBIAL%20PRODUCTION%20OF%20%28ABSTRACT%29.pdf Wen Chian, Liew (2020) Microbial Production Of Polyhydroxybutyrate (Phb) By Burkholderia Cepacia Bpt1213 From Different Carbon Sources And Its Fermentation Kinetics. Project Report. Pusat Pengajian Teknologi Industri. (Submitted) http://ethesis.usm.my:8080/jspui/
spellingShingle T1-995 Technology(General)
Wen Chian, Liew
Microbial Production Of Polyhydroxybutyrate (Phb) By Burkholderia Cepacia Bpt1213 From Different Carbon Sources And Its Fermentation Kinetics
title Microbial Production Of Polyhydroxybutyrate (Phb) By Burkholderia Cepacia Bpt1213 From Different Carbon Sources And Its Fermentation Kinetics
title_full Microbial Production Of Polyhydroxybutyrate (Phb) By Burkholderia Cepacia Bpt1213 From Different Carbon Sources And Its Fermentation Kinetics
title_fullStr Microbial Production Of Polyhydroxybutyrate (Phb) By Burkholderia Cepacia Bpt1213 From Different Carbon Sources And Its Fermentation Kinetics
title_full_unstemmed Microbial Production Of Polyhydroxybutyrate (Phb) By Burkholderia Cepacia Bpt1213 From Different Carbon Sources And Its Fermentation Kinetics
title_short Microbial Production Of Polyhydroxybutyrate (Phb) By Burkholderia Cepacia Bpt1213 From Different Carbon Sources And Its Fermentation Kinetics
title_sort microbial production of polyhydroxybutyrate phb by burkholderia cepacia bpt1213 from different carbon sources and its fermentation kinetics
topic T1-995 Technology(General)
url http://eprints.usm.my/46844/1/MICROBIAL%20PRODUCTION%20OF%20%28ABSTRACT%29.pdf
work_keys_str_mv AT wenchianliew microbialproductionofpolyhydroxybutyratephbbyburkholderiacepaciabpt1213fromdifferentcarbonsourcesanditsfermentationkinetics