Production Of Novel Recombinant Anti-PFHRP2 VNAR-G1 Protein Using Escherichia COLI BL21(DE3) Expression System
Malaria rapid diagnostic tests (RDTs) act as important antibody-based immunoassays for prompt malaria diagnosis. Conventional monoclonal antibodies (mAbs) are widely used in RDTs but it can be easily degraded at high ambient temperatures. Hence, the shark VNARS might be good alternatives to mAbs...
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| Format: | Monograph |
| Language: | English |
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Pusat Pengajian Teknologi Industri
2020
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| Online Access: | http://eprints.usm.my/46854/1/PRODUCTION%20OF%20NOVEL%20RECOMBINANT%20ANTIPFHRP%20%28ABSTRACT%29.pdf |
| _version_ | 1825905636039196672 |
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| author | Kok, Boon Hui |
| author_facet | Kok, Boon Hui |
| author_sort | Kok, Boon Hui |
| collection | USM |
| description | Malaria rapid diagnostic tests (RDTs) act as important antibody-based immunoassays
for prompt malaria diagnosis. Conventional monoclonal antibodies (mAbs) are
widely used in RDTs but it can be easily degraded at high ambient temperatures.
Hence, the shark VNARS might be good alternatives to mAbs due to its higher thermal
stability and binding affinity with antigens. In this study, the recombinant antiPfHRP2 VNAR-G1 protein was produced in E. coli expression system through various
steps such as recombinant cell isolation, PCR, agarose gel electrophoresis, plasmid
extraction, transformation and protein expression. |
| first_indexed | 2024-03-06T15:37:25Z |
| format | Monograph |
| id | usm.eprints-46854 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T15:37:25Z |
| publishDate | 2020 |
| publisher | Pusat Pengajian Teknologi Industri |
| record_format | dspace |
| spelling | usm.eprints-468542020-08-27T02:09:42Z http://eprints.usm.my/46854/ Production Of Novel Recombinant Anti-PFHRP2 VNAR-G1 Protein Using Escherichia COLI BL21(DE3) Expression System Kok, Boon Hui T1-995 Technology(General) Malaria rapid diagnostic tests (RDTs) act as important antibody-based immunoassays for prompt malaria diagnosis. Conventional monoclonal antibodies (mAbs) are widely used in RDTs but it can be easily degraded at high ambient temperatures. Hence, the shark VNARS might be good alternatives to mAbs due to its higher thermal stability and binding affinity with antigens. In this study, the recombinant antiPfHRP2 VNAR-G1 protein was produced in E. coli expression system through various steps such as recombinant cell isolation, PCR, agarose gel electrophoresis, plasmid extraction, transformation and protein expression. Pusat Pengajian Teknologi Industri 2020-06 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/46854/1/PRODUCTION%20OF%20NOVEL%20RECOMBINANT%20ANTIPFHRP%20%28ABSTRACT%29.pdf Kok, Boon Hui (2020) Production Of Novel Recombinant Anti-PFHRP2 VNAR-G1 Protein Using Escherichia COLI BL21(DE3) Expression System. Project Report. Pusat Pengajian Teknologi Industri. (Submitted) http://ethesis.usm.my:8080/jspui/ |
| spellingShingle | T1-995 Technology(General) Kok, Boon Hui Production Of Novel Recombinant Anti-PFHRP2 VNAR-G1 Protein Using Escherichia COLI BL21(DE3) Expression System |
| title | Production Of Novel Recombinant Anti-PFHRP2 VNAR-G1 Protein Using Escherichia
COLI BL21(DE3) Expression System |
| title_full | Production Of Novel Recombinant Anti-PFHRP2 VNAR-G1 Protein Using Escherichia
COLI BL21(DE3) Expression System |
| title_fullStr | Production Of Novel Recombinant Anti-PFHRP2 VNAR-G1 Protein Using Escherichia
COLI BL21(DE3) Expression System |
| title_full_unstemmed | Production Of Novel Recombinant Anti-PFHRP2 VNAR-G1 Protein Using Escherichia
COLI BL21(DE3) Expression System |
| title_short | Production Of Novel Recombinant Anti-PFHRP2 VNAR-G1 Protein Using Escherichia
COLI BL21(DE3) Expression System |
| title_sort | production of novel recombinant anti pfhrp2 vnar g1 protein using escherichia coli bl21 de3 expression system |
| topic | T1-995 Technology(General) |
| url | http://eprints.usm.my/46854/1/PRODUCTION%20OF%20NOVEL%20RECOMBINANT%20ANTIPFHRP%20%28ABSTRACT%29.pdf |
| work_keys_str_mv | AT kokboonhui productionofnovelrecombinantantipfhrp2vnarg1proteinusingescherichiacolibl21de3expressionsystem |