Physical Extraction Of Flagellavia Ultrasonication From Bacillus Subtilis 168 With Various Control Elements

Flagella was commonly known as the tail of bacteria. The major structural protein of bacterial flagella is called flagellin. Flagella have a potential to become biodegradable nanotube and important for drug delivery, electronic or semiconductor. There are still lack of research about flagella sep...

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主要作者: Yan Leong, Kah
格式: Monograph
语言:English
出版: Pusat Pengajian Teknologi Industri 2020
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在线阅读:http://eprints.usm.my/46941/1/PHYSICAL%20EXTRACTION%20OF%20FLAGELLAVIA%20%28ABSTRACT%29.pdf
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author Yan Leong, Kah
author_facet Yan Leong, Kah
author_sort Yan Leong, Kah
collection USM
description Flagella was commonly known as the tail of bacteria. The major structural protein of bacterial flagella is called flagellin. Flagella have a potential to become biodegradable nanotube and important for drug delivery, electronic or semiconductor. There are still lack of research about flagella separation and purification. In this research, the morphology of the flagella and basal body for Bacillus subtilis 168 was investigated by scanning electron microscope and transmission electron microscope. The characterization of flagella was found that difference between flagella and cell body is nitrogen (N), magnesium (Mg) and Sulphur (S) by energy-dispersive X-ray spectroscopy. Statistically designed experiment revealed significant effects of sonication duration and sonicator output power on the concentration of flagellin after sonication. The cell body of Bacillus subtilis 168 was started to breaked from 20 % sonicator output power.
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institution Universiti Sains Malaysia
language English
last_indexed 2024-03-06T15:37:38Z
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spelling usm.eprints-469412020-08-27T01:49:45Z http://eprints.usm.my/46941/ Physical Extraction Of Flagellavia Ultrasonication From Bacillus Subtilis 168 With Various Control Elements Yan Leong, Kah T1-995 Technology(General) Flagella was commonly known as the tail of bacteria. The major structural protein of bacterial flagella is called flagellin. Flagella have a potential to become biodegradable nanotube and important for drug delivery, electronic or semiconductor. There are still lack of research about flagella separation and purification. In this research, the morphology of the flagella and basal body for Bacillus subtilis 168 was investigated by scanning electron microscope and transmission electron microscope. The characterization of flagella was found that difference between flagella and cell body is nitrogen (N), magnesium (Mg) and Sulphur (S) by energy-dispersive X-ray spectroscopy. Statistically designed experiment revealed significant effects of sonication duration and sonicator output power on the concentration of flagellin after sonication. The cell body of Bacillus subtilis 168 was started to breaked from 20 % sonicator output power. Pusat Pengajian Teknologi Industri 2020-06 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/46941/1/PHYSICAL%20EXTRACTION%20OF%20FLAGELLAVIA%20%28ABSTRACT%29.pdf Yan Leong, Kah (2020) Physical Extraction Of Flagellavia Ultrasonication From Bacillus Subtilis 168 With Various Control Elements. Project Report. Pusat Pengajian Teknologi Industri. (Submitted) http://ethesis.usm.my:8080/jspui/
spellingShingle T1-995 Technology(General)
Yan Leong, Kah
Physical Extraction Of Flagellavia Ultrasonication From Bacillus Subtilis 168 With Various Control Elements
title Physical Extraction Of Flagellavia Ultrasonication From Bacillus Subtilis 168 With Various Control Elements
title_full Physical Extraction Of Flagellavia Ultrasonication From Bacillus Subtilis 168 With Various Control Elements
title_fullStr Physical Extraction Of Flagellavia Ultrasonication From Bacillus Subtilis 168 With Various Control Elements
title_full_unstemmed Physical Extraction Of Flagellavia Ultrasonication From Bacillus Subtilis 168 With Various Control Elements
title_short Physical Extraction Of Flagellavia Ultrasonication From Bacillus Subtilis 168 With Various Control Elements
title_sort physical extraction of flagellavia ultrasonication from bacillus subtilis 168 with various control elements
topic T1-995 Technology(General)
url http://eprints.usm.my/46941/1/PHYSICAL%20EXTRACTION%20OF%20FLAGELLAVIA%20%28ABSTRACT%29.pdf
work_keys_str_mv AT yanleongkah physicalextractionofflagellaviaultrasonicationfrombacillussubtilis168withvariouscontrolelements