Biosynthesis Of Poly(3-hydroxybutyrate) By Cupriaviadus Necator Dsm 428 Utilizing Waste Date Fruits As A Carbon Source
Many researches have been conducted to find a replacement for conventional petroleumderived plastics. Poly(3-hydroxybutyrate) (PHB) is biodegradable polymers produced from a renewable source have gained many interest to replace conventional plastic, as it possesses excellent chemical and physical...
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| Format: | Monograph |
| Language: | English |
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Pusat Pengajian Teknologi Industri
2020
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| Online Access: | http://eprints.usm.my/46957/1/BIOSYNTHESIS%20OF%20POLY%283-HYDROXYBUTYRATE%29%20%28ABSTRACT%29.pdf |
| _version_ | 1797011670627778560 |
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| author | Nur Aqilah Abdul Wahab, Siti |
| author_facet | Nur Aqilah Abdul Wahab, Siti |
| author_sort | Nur Aqilah Abdul Wahab, Siti |
| collection | USM |
| description | Many researches have been conducted to find a replacement for conventional petroleumderived plastics. Poly(3-hydroxybutyrate) (PHB) is biodegradable polymers produced from a
renewable source have gained many interest to replace conventional plastic, as it possesses
excellent chemical and physical properties, with an advantages of complete biodegradability.
The limitation in producing huge amount of PHB is due to its expensive cost of carbon
sources. This study focus on the production of PHB by Cupriavidus necator using low-cost
carbon sources such as waste of date fruits. Three concentration ratios of date fruits to water
was prepared which are 1:3, 1:5, and 1:8 with the fermentation condition of 30℃, 200 rpm
and up to 29 hours of fermentation. Analysis of sugar content in date fruits solution was
performed prior and after the fermentation process. Glucose content shows the highest
amount compared to fructose and sucrose which are 48.5 ± 6.3%, 55.7 ± 3.8% and 60.6 ±
5.1%. The average CDW of ratio 1:3, 1:5 and 1:8 are 0.179 ± 0.03g/L, 0.166 ± 0.01g/L and
0.180 ± 0.01g/L. While the PHB content are, 7.9± 3.0%, 4.1± 3.4% and 10.4± 9.6%
respectively. Ratio 1:8 state the highest CDW and PHB in comparison to all ratios even it is
the most diluted date fruits solution. This observation is due to effective utilization of carbon
sources as it contained the highest glucose compared to other ratios, and limited amount of
nitrogen (1.45 ± 1.06%) which enhanced the positive effect on PHB production. |
| first_indexed | 2024-03-06T15:37:41Z |
| format | Monograph |
| id | usm.eprints-46957 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T15:37:41Z |
| publishDate | 2020 |
| publisher | Pusat Pengajian Teknologi Industri |
| record_format | dspace |
| spelling | usm.eprints-469572020-08-27T01:42:26Z http://eprints.usm.my/46957/ Biosynthesis Of Poly(3-hydroxybutyrate) By Cupriaviadus Necator Dsm 428 Utilizing Waste Date Fruits As A Carbon Source Nur Aqilah Abdul Wahab, Siti T1-995 Technology(General) Many researches have been conducted to find a replacement for conventional petroleumderived plastics. Poly(3-hydroxybutyrate) (PHB) is biodegradable polymers produced from a renewable source have gained many interest to replace conventional plastic, as it possesses excellent chemical and physical properties, with an advantages of complete biodegradability. The limitation in producing huge amount of PHB is due to its expensive cost of carbon sources. This study focus on the production of PHB by Cupriavidus necator using low-cost carbon sources such as waste of date fruits. Three concentration ratios of date fruits to water was prepared which are 1:3, 1:5, and 1:8 with the fermentation condition of 30℃, 200 rpm and up to 29 hours of fermentation. Analysis of sugar content in date fruits solution was performed prior and after the fermentation process. Glucose content shows the highest amount compared to fructose and sucrose which are 48.5 ± 6.3%, 55.7 ± 3.8% and 60.6 ± 5.1%. The average CDW of ratio 1:3, 1:5 and 1:8 are 0.179 ± 0.03g/L, 0.166 ± 0.01g/L and 0.180 ± 0.01g/L. While the PHB content are, 7.9± 3.0%, 4.1± 3.4% and 10.4± 9.6% respectively. Ratio 1:8 state the highest CDW and PHB in comparison to all ratios even it is the most diluted date fruits solution. This observation is due to effective utilization of carbon sources as it contained the highest glucose compared to other ratios, and limited amount of nitrogen (1.45 ± 1.06%) which enhanced the positive effect on PHB production. Pusat Pengajian Teknologi Industri 2020-06 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/46957/1/BIOSYNTHESIS%20OF%20POLY%283-HYDROXYBUTYRATE%29%20%28ABSTRACT%29.pdf Nur Aqilah Abdul Wahab, Siti (2020) Biosynthesis Of Poly(3-hydroxybutyrate) By Cupriaviadus Necator Dsm 428 Utilizing Waste Date Fruits As A Carbon Source. Project Report. Pusat Pengajian Teknologi Industri. (Submitted) http://ethesis.usm.my:8080/jspui/ |
| spellingShingle | T1-995 Technology(General) Nur Aqilah Abdul Wahab, Siti Biosynthesis Of Poly(3-hydroxybutyrate) By Cupriaviadus Necator Dsm 428 Utilizing Waste Date Fruits As A Carbon Source |
| title | Biosynthesis Of Poly(3-hydroxybutyrate)
By Cupriaviadus Necator Dsm 428 Utilizing
Waste Date Fruits As A Carbon Source |
| title_full | Biosynthesis Of Poly(3-hydroxybutyrate)
By Cupriaviadus Necator Dsm 428 Utilizing
Waste Date Fruits As A Carbon Source |
| title_fullStr | Biosynthesis Of Poly(3-hydroxybutyrate)
By Cupriaviadus Necator Dsm 428 Utilizing
Waste Date Fruits As A Carbon Source |
| title_full_unstemmed | Biosynthesis Of Poly(3-hydroxybutyrate)
By Cupriaviadus Necator Dsm 428 Utilizing
Waste Date Fruits As A Carbon Source |
| title_short | Biosynthesis Of Poly(3-hydroxybutyrate)
By Cupriaviadus Necator Dsm 428 Utilizing
Waste Date Fruits As A Carbon Source |
| title_sort | biosynthesis of poly 3 hydroxybutyrate by cupriaviadus necator dsm 428 utilizing waste date fruits as a carbon source |
| topic | T1-995 Technology(General) |
| url | http://eprints.usm.my/46957/1/BIOSYNTHESIS%20OF%20POLY%283-HYDROXYBUTYRATE%29%20%28ABSTRACT%29.pdf |
| work_keys_str_mv | AT nuraqilahabdulwahabsiti biosynthesisofpoly3hydroxybutyratebycupriaviadusnecatordsm428utilizingwastedatefruitsasacarbonsource |