Minichromosome Maintenance Complex (MCM) Genes Profiling and MCM2 Protein Expression in Cervical Cancer Development
Objective: Minichromosome maintenance complex (MCM) proteins are essential for the process of DNA replication and cell division. This study aimed to evaluate MCM genes expression profiles and MCM2 protein in HPV-associated cervical carcinogenesis. Methodology: MCM2, 4, 5 and 7 genes expression pro...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Asia Pacific Organization for Cancer Prevention (APOCP)
2019
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| Online Access: | http://eprints.usm.my/47610/1/APJCP_Volume%2020_Issue%2010_Pages%203043-3049.pdf |
| _version_ | 1797011796407615488 |
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| author | Kaur, Gurjeet Balasubramaniam, Shandra Devi Yung, Jen Lee Balakrishnan, Venugopal Chern, Ein Oon |
| author_facet | Kaur, Gurjeet Balasubramaniam, Shandra Devi Yung, Jen Lee Balakrishnan, Venugopal Chern, Ein Oon |
| author_sort | Kaur, Gurjeet |
| collection | USM |
| description | Objective: Minichromosome maintenance complex (MCM) proteins are essential for the process of DNA replication
and cell division. This study aimed to evaluate MCM genes expression profiles and MCM2 protein in HPV-associated
cervical carcinogenesis. Methodology: MCM2, 4, 5 and 7 genes expression profiles were evaluated in three cervical
tissue samples each of normal cervix, human papillomavirus (HPV)-infected low grade squamous intraepithelial
lesion (LSIL), high grade squamous intraepithelial lesion (HSIL) and squamous cell carcinoma (SCC), using Human
Transcriptome Array 2.0 and validated by nCounter® PanCancer Pathway NanoString Array. Immunohistochemical
expression of MCM2 protein was semi-quantitatively assessed by histoscore in tissue microarrays containing 9 cases of
normal cervix, 10 LSIL, 10 HSIL and 42 cases of SCC. Results: MCM2, 4, 5 and 7 genes expressions were upregulated
with increasing fold change during the progression from LSIL to HSIL and the highest in SCC. MCM2 gene had the
highest fold change in SCC compared to normal cervix. Immunohistochemically, MCM2 protein was localised in
the nuclei of basal cells of normal cervical epithelium and dysplastic-neoplastic cells of CIN and SCC. There was a
significant difference in MCM2 protein expression between the histological groups (P = 0.039), and histoscore was the
highest in HSIL compared to normal cervix (P = 0.010). Conclusion: The upregulation of MCM genes expressions in
cervical carcinogenesis reaffirms MCM as a proliferative marker in DNA replication pathway, whereby proliferation of
dysplastic and cancer cells become increasingly dysregulated and uncontrolled. A strong expression of MCM2 protein
in HSIL may aid as a concatenated screening tool in detecting pre-cancerous cervical lesions. |
| first_indexed | 2024-03-06T15:39:33Z |
| format | Article |
| id | usm.eprints-47610 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T15:39:33Z |
| publishDate | 2019 |
| publisher | Asia Pacific Organization for Cancer Prevention (APOCP) |
| record_format | dspace |
| spelling | usm.eprints-476102020-10-30T20:58:52Z http://eprints.usm.my/47610/ Minichromosome Maintenance Complex (MCM) Genes Profiling and MCM2 Protein Expression in Cervical Cancer Development Kaur, Gurjeet Balasubramaniam, Shandra Devi Yung, Jen Lee Balakrishnan, Venugopal Chern, Ein Oon RC254-282 Neoplasms. Tumors. Oncology (including Cancer) Objective: Minichromosome maintenance complex (MCM) proteins are essential for the process of DNA replication and cell division. This study aimed to evaluate MCM genes expression profiles and MCM2 protein in HPV-associated cervical carcinogenesis. Methodology: MCM2, 4, 5 and 7 genes expression profiles were evaluated in three cervical tissue samples each of normal cervix, human papillomavirus (HPV)-infected low grade squamous intraepithelial lesion (LSIL), high grade squamous intraepithelial lesion (HSIL) and squamous cell carcinoma (SCC), using Human Transcriptome Array 2.0 and validated by nCounter® PanCancer Pathway NanoString Array. Immunohistochemical expression of MCM2 protein was semi-quantitatively assessed by histoscore in tissue microarrays containing 9 cases of normal cervix, 10 LSIL, 10 HSIL and 42 cases of SCC. Results: MCM2, 4, 5 and 7 genes expressions were upregulated with increasing fold change during the progression from LSIL to HSIL and the highest in SCC. MCM2 gene had the highest fold change in SCC compared to normal cervix. Immunohistochemically, MCM2 protein was localised in the nuclei of basal cells of normal cervical epithelium and dysplastic-neoplastic cells of CIN and SCC. There was a significant difference in MCM2 protein expression between the histological groups (P = 0.039), and histoscore was the highest in HSIL compared to normal cervix (P = 0.010). Conclusion: The upregulation of MCM genes expressions in cervical carcinogenesis reaffirms MCM as a proliferative marker in DNA replication pathway, whereby proliferation of dysplastic and cancer cells become increasingly dysregulated and uncontrolled. A strong expression of MCM2 protein in HSIL may aid as a concatenated screening tool in detecting pre-cancerous cervical lesions. Asia Pacific Organization for Cancer Prevention (APOCP) 2019 Article PeerReviewed application/pdf en http://eprints.usm.my/47610/1/APJCP_Volume%2020_Issue%2010_Pages%203043-3049.pdf Kaur, Gurjeet and Balasubramaniam, Shandra Devi and Yung, Jen Lee and Balakrishnan, Venugopal and Chern, Ein Oon (2019) Minichromosome Maintenance Complex (MCM) Genes Profiling and MCM2 Protein Expression in Cervical Cancer Development. Asian Pacific Journal of Cancer Prevention, 20 (10). pp. 3043-3049. ISSN 2476-762X |
| spellingShingle | RC254-282 Neoplasms. Tumors. Oncology (including Cancer) Kaur, Gurjeet Balasubramaniam, Shandra Devi Yung, Jen Lee Balakrishnan, Venugopal Chern, Ein Oon Minichromosome Maintenance Complex (MCM) Genes Profiling and MCM2 Protein Expression in Cervical Cancer Development |
| title | Minichromosome Maintenance Complex (MCM) Genes Profiling
and MCM2 Protein Expression in Cervical Cancer Development |
| title_full | Minichromosome Maintenance Complex (MCM) Genes Profiling
and MCM2 Protein Expression in Cervical Cancer Development |
| title_fullStr | Minichromosome Maintenance Complex (MCM) Genes Profiling
and MCM2 Protein Expression in Cervical Cancer Development |
| title_full_unstemmed | Minichromosome Maintenance Complex (MCM) Genes Profiling
and MCM2 Protein Expression in Cervical Cancer Development |
| title_short | Minichromosome Maintenance Complex (MCM) Genes Profiling
and MCM2 Protein Expression in Cervical Cancer Development |
| title_sort | minichromosome maintenance complex mcm genes profiling and mcm2 protein expression in cervical cancer development |
| topic | RC254-282 Neoplasms. Tumors. Oncology (including Cancer) |
| url | http://eprints.usm.my/47610/1/APJCP_Volume%2020_Issue%2010_Pages%203043-3049.pdf |
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