Production, Characterization And Identification Of Bioactive Peptides From Annona Squamosa L. Seeds
Annona squamosa L. seed protein isolate (ASPI) and defatted protein isolate (DASPI) were successfully isolated using phosphate buffer at pH 8 followed by acidic precipitation at pH 4.5. The predominant proteins found in the seeds were β-conglycinin and glycinin. The amino acid profiles showed that t...
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| Format: | Thesis |
| Language: | English |
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2017
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| Online Access: | http://eprints.usm.my/47798/1/PRODUCTION%2C%20CHARACTERIZATION%20AND.pdf%20cut.pdf |
| _version_ | 1797011832372723712 |
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| author | Sofian, Nurul Hidayatul Afifah Saufi |
| author_facet | Sofian, Nurul Hidayatul Afifah Saufi |
| author_sort | Sofian, Nurul Hidayatul Afifah Saufi |
| collection | USM |
| description | Annona squamosa L. seed protein isolate (ASPI) and defatted protein isolate (DASPI) were successfully isolated using phosphate buffer at pH 8 followed by acidic precipitation at pH 4.5. The predominant proteins found in the seeds were β-conglycinin and glycinin. The amino acid profiles showed that the protein isolates contained a high amount of glutamic acid, glycine, aspartic acid, and leucine with essential to total amino acid ratio of 0.4. The Fourier transform infrared (FTIR) spectra showed that the isolated proteins exhibited a similar functional group of protein, which was extracted from legumes, and the isoelectric point (pI) was found at pH 3-4. The functionalities of both protein isolates were also investigated. The bioactivities were evaluated based on 2,2-diphenyl-1-picrylhydrazyl scavenging activity (DPPH), ferric reducing antioxidant power (FRAP), α-amylase, ACE inhibitory and lipase inhibitory activity assays. |
| first_indexed | 2024-03-06T15:40:05Z |
| format | Thesis |
| id | usm.eprints-47798 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T15:40:05Z |
| publishDate | 2017 |
| record_format | dspace |
| spelling | usm.eprints-477982020-10-28T01:04:28Z http://eprints.usm.my/47798/ Production, Characterization And Identification Of Bioactive Peptides From Annona Squamosa L. Seeds Sofian, Nurul Hidayatul Afifah Saufi QK1-989 Botany Annona squamosa L. seed protein isolate (ASPI) and defatted protein isolate (DASPI) were successfully isolated using phosphate buffer at pH 8 followed by acidic precipitation at pH 4.5. The predominant proteins found in the seeds were β-conglycinin and glycinin. The amino acid profiles showed that the protein isolates contained a high amount of glutamic acid, glycine, aspartic acid, and leucine with essential to total amino acid ratio of 0.4. The Fourier transform infrared (FTIR) spectra showed that the isolated proteins exhibited a similar functional group of protein, which was extracted from legumes, and the isoelectric point (pI) was found at pH 3-4. The functionalities of both protein isolates were also investigated. The bioactivities were evaluated based on 2,2-diphenyl-1-picrylhydrazyl scavenging activity (DPPH), ferric reducing antioxidant power (FRAP), α-amylase, ACE inhibitory and lipase inhibitory activity assays. 2017-12 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/47798/1/PRODUCTION%2C%20CHARACTERIZATION%20AND.pdf%20cut.pdf Sofian, Nurul Hidayatul Afifah Saufi (2017) Production, Characterization And Identification Of Bioactive Peptides From Annona Squamosa L. Seeds. Masters thesis, Universiti Sains Malaysia. |
| spellingShingle | QK1-989 Botany Sofian, Nurul Hidayatul Afifah Saufi Production, Characterization And Identification Of Bioactive Peptides From Annona Squamosa L. Seeds |
| title | Production, Characterization And Identification Of Bioactive Peptides From Annona Squamosa L. Seeds |
| title_full | Production, Characterization And Identification Of Bioactive Peptides From Annona Squamosa L. Seeds |
| title_fullStr | Production, Characterization And Identification Of Bioactive Peptides From Annona Squamosa L. Seeds |
| title_full_unstemmed | Production, Characterization And Identification Of Bioactive Peptides From Annona Squamosa L. Seeds |
| title_short | Production, Characterization And Identification Of Bioactive Peptides From Annona Squamosa L. Seeds |
| title_sort | production characterization and identification of bioactive peptides from annona squamosa l seeds |
| topic | QK1-989 Botany |
| url | http://eprints.usm.my/47798/1/PRODUCTION%2C%20CHARACTERIZATION%20AND.pdf%20cut.pdf |
| work_keys_str_mv | AT sofiannurulhidayatulafifahsaufi productioncharacterizationandidentificationofbioactivepeptidesfromannonasquamosalseeds |