Screening of potential microorganisms for the production of novel cyclodextrin glycosyltransferase (CGTase)enzyme
Ten soil samples were collected from the vicinity of restaurant's drain where the wastewater contain remaining starch food constituent was filtered and drained away, housing estate ditch and trash waste disposal area around Kubang Kerian. They were screened for potential CGTase enzyme produc...
| Main Author: | |
|---|---|
| Format: | Monograph |
| Language: | English |
| Published: |
Pusat Pengajian Sains Perubatan Universiti Sains Malaysia
2008
|
| Subjects: | |
| Online Access: | http://eprints.usm.my/50597/1/MOHD%20NAZRUL%20ANUAR%20BIN%20ALI%20-%2024%20pages.pdf |
| _version_ | 1797012455559266304 |
|---|---|
| author | Nazrul Anuar, Ali |
| author_facet | Nazrul Anuar, Ali |
| author_sort | Nazrul Anuar, Ali |
| collection | USM |
| description | Ten soil samples were collected from the vicinity of restaurant's drain where the wastewater
contain remaining starch food constituent was filtered and drained away, housing estate ditch and
trash waste disposal area around Kubang Kerian. They were screened for potential CGTase
enzyme producer by culturing in media containing cassava starch and nutrient agar. A total of 40
colonies have been successfully isolated in this study by detecting the production of clearance
zone around them, and those colonies were selected as CGTase producing bacteria. The CGTase
producing bacteria were identified as Bacillus species by microscopy observation. The bacteria
was grown in production medium containing 1.5% (w/v) cassava starch, 0.4% (w/v) (NH4)2S04,
O.lM phosphate buffer (pH 7.0), 0.002% (w/v) MgS04 and 0.002% (w/v) FeS04 for the
production of CGTase enzyme. The supernatant obtained after centrifugation at 3000 g for 15
minutes was used as crude enzyme. The activity of CGTase production was evaluated
qualitatively by observing the production of clearance zone around the colony and quantitatively
by determining the dextrinizing activity based on the method described by Fuwa, (1954) and
Matsuzawa et al., (1975) with slight modification. |
| first_indexed | 2024-03-06T15:47:57Z |
| format | Monograph |
| id | usm.eprints-50597 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T15:47:57Z |
| publishDate | 2008 |
| publisher | Pusat Pengajian Sains Perubatan Universiti Sains Malaysia |
| record_format | dspace |
| spelling | usm.eprints-505972021-11-15T04:41:33Z http://eprints.usm.my/50597/ Screening of potential microorganisms for the production of novel cyclodextrin glycosyltransferase (CGTase)enzyme Nazrul Anuar, Ali R Medicine (General) Ten soil samples were collected from the vicinity of restaurant's drain where the wastewater contain remaining starch food constituent was filtered and drained away, housing estate ditch and trash waste disposal area around Kubang Kerian. They were screened for potential CGTase enzyme producer by culturing in media containing cassava starch and nutrient agar. A total of 40 colonies have been successfully isolated in this study by detecting the production of clearance zone around them, and those colonies were selected as CGTase producing bacteria. The CGTase producing bacteria were identified as Bacillus species by microscopy observation. The bacteria was grown in production medium containing 1.5% (w/v) cassava starch, 0.4% (w/v) (NH4)2S04, O.lM phosphate buffer (pH 7.0), 0.002% (w/v) MgS04 and 0.002% (w/v) FeS04 for the production of CGTase enzyme. The supernatant obtained after centrifugation at 3000 g for 15 minutes was used as crude enzyme. The activity of CGTase production was evaluated qualitatively by observing the production of clearance zone around the colony and quantitatively by determining the dextrinizing activity based on the method described by Fuwa, (1954) and Matsuzawa et al., (1975) with slight modification. Pusat Pengajian Sains Perubatan Universiti Sains Malaysia 2008 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/50597/1/MOHD%20NAZRUL%20ANUAR%20BIN%20ALI%20-%2024%20pages.pdf Nazrul Anuar, Ali (2008) Screening of potential microorganisms for the production of novel cyclodextrin glycosyltransferase (CGTase)enzyme. Other. Pusat Pengajian Sains Perubatan Universiti Sains Malaysia. (Submitted) |
| spellingShingle | R Medicine (General) Nazrul Anuar, Ali Screening of potential microorganisms for the production of novel cyclodextrin glycosyltransferase (CGTase)enzyme |
| title | Screening of potential microorganisms for the
production of novel cyclodextrin
glycosyltransferase (CGTase)enzyme |
| title_full | Screening of potential microorganisms for the
production of novel cyclodextrin
glycosyltransferase (CGTase)enzyme |
| title_fullStr | Screening of potential microorganisms for the
production of novel cyclodextrin
glycosyltransferase (CGTase)enzyme |
| title_full_unstemmed | Screening of potential microorganisms for the
production of novel cyclodextrin
glycosyltransferase (CGTase)enzyme |
| title_short | Screening of potential microorganisms for the
production of novel cyclodextrin
glycosyltransferase (CGTase)enzyme |
| title_sort | screening of potential microorganisms for the production of novel cyclodextrin glycosyltransferase cgtase enzyme |
| topic | R Medicine (General) |
| url | http://eprints.usm.my/50597/1/MOHD%20NAZRUL%20ANUAR%20BIN%20ALI%20-%2024%20pages.pdf |
| work_keys_str_mv | AT nazrulanuarali screeningofpotentialmicroorganismsfortheproductionofnovelcyclodextringlycosyltransferasecgtaseenzyme |