Touch dna profiling from processed fingerprint

Forensics identification using fingerprint analysis is only possible if there is a complete pattern of ridges obtained from the crime scene. In this study we have attempted to perfonn DNA analysis on processed smudged fingerprints. A subject was requested to deposit complete and portioning finger...

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Bibliographic Details
Main Author: Hafidza, Baharum
Format: Monograph
Language:English
Published: Pusat Pengajian Sains Perubatan Universiti Sains Malaysia 2009
Subjects:
Online Access:http://eprints.usm.my/51113/1/HAFIDZAH%20BINTI%20BAHARUM%20-%2024%20pages.pdf
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author Hafidza, Baharum
author_facet Hafidza, Baharum
author_sort Hafidza, Baharum
collection USM
description Forensics identification using fingerprint analysis is only possible if there is a complete pattern of ridges obtained from the crime scene. In this study we have attempted to perfonn DNA analysis on processed smudged fingerprints. A subject was requested to deposit complete and portioning fingerprints on glass surface. The deposited fingerprints were exposed to 18 •c, 28 OC, 34 •c, and 40 OC for an hour, 3 days, and 7 days. The fingerprints were then visualized using black powder, were measured and one of sample was recovered using tape lifting method. A total of 2t.11 of the extracted DNA was quantitated using Real Time PCR (Quantifiler kit). 2 samples with DNA concentration above 1.0 ng was used for amplification using Amp Ff.STR ldentifiler kit and another 2 samples concentration above 0.2 ng was used for amplification using AmpFtSTR Minifiler kit. Both kits failed to amplify with DNA and therefore optimization is required for the collection and extraction methods used in this study.
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spelling usm.eprints-511132022-01-10T04:30:53Z http://eprints.usm.my/51113/ Touch dna profiling from processed fingerprint Hafidza, Baharum R Medicine (General) Forensics identification using fingerprint analysis is only possible if there is a complete pattern of ridges obtained from the crime scene. In this study we have attempted to perfonn DNA analysis on processed smudged fingerprints. A subject was requested to deposit complete and portioning fingerprints on glass surface. The deposited fingerprints were exposed to 18 •c, 28 OC, 34 •c, and 40 OC for an hour, 3 days, and 7 days. The fingerprints were then visualized using black powder, were measured and one of sample was recovered using tape lifting method. A total of 2t.11 of the extracted DNA was quantitated using Real Time PCR (Quantifiler kit). 2 samples with DNA concentration above 1.0 ng was used for amplification using Amp Ff.STR ldentifiler kit and another 2 samples concentration above 0.2 ng was used for amplification using AmpFtSTR Minifiler kit. Both kits failed to amplify with DNA and therefore optimization is required for the collection and extraction methods used in this study. Pusat Pengajian Sains Perubatan Universiti Sains Malaysia 2009 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/51113/1/HAFIDZAH%20BINTI%20BAHARUM%20-%2024%20pages.pdf Hafidza, Baharum (2009) Touch dna profiling from processed fingerprint. Other. Pusat Pengajian Sains Perubatan Universiti Sains Malaysia. (Submitted)
spellingShingle R Medicine (General)
Hafidza, Baharum
Touch dna profiling from processed fingerprint
title Touch dna profiling from processed fingerprint
title_full Touch dna profiling from processed fingerprint
title_fullStr Touch dna profiling from processed fingerprint
title_full_unstemmed Touch dna profiling from processed fingerprint
title_short Touch dna profiling from processed fingerprint
title_sort touch dna profiling from processed fingerprint
topic R Medicine (General)
url http://eprints.usm.my/51113/1/HAFIDZAH%20BINTI%20BAHARUM%20-%2024%20pages.pdf
work_keys_str_mv AT hafidzabaharum touchdnaprofilingfromprocessedfingerprint