Distribution of allele frequencies of short tandem repeats (STRs) DI6S539, D7S820 and D13S317 in random temiar population group of Malaysia
Polymerase Chain Reaction based Short Tandem Repeat (PCR-STR) method provides an unprecedented DNA profiling technique for forensic community in identification and individualization purposes. In the analysis of biological material using DNA profiling technique, the information regarding the distr...
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| Format: | Monograph |
| Language: | English |
| Published: |
Universiti Sains Malaysia
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| Online Access: | http://eprints.usm.my/51130/1/KHOR%20POK%20PIN%20-%2024%20pages.pdf |
| _version_ | 1825906428630532096 |
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| author | Khor Kok, Pin |
| author_facet | Khor Kok, Pin |
| author_sort | Khor Kok, Pin |
| collection | USM |
| description | Polymerase Chain Reaction based Short Tandem Repeat (PCR-STR) method provides an
unprecedented DNA profiling technique for forensic community in identification and
individualization purposes. In the analysis of biological material using DNA profiling
technique, the information regarding the distribution of allele frequency in a defmed
population is mandatory. This particularly necessary in paternity testing that involves
compilation of Paternity Index (PI), in which the allele frequency is required to indicate
whether the alleged father is the true biological father of the child. Validated 8TR loci have
been analysed extensively in various populations for forensic application. In this research
study, population genetic studies were carried out for three 8TR loci, namely D168539,
D7S820 and D13S317 for Temiar population by using a multiplex PCR reaction followed
by 4% polyacrylamide gel electrophoresis (PAGE) and silver staining. The heterozygosity
(H) for these three loci, i.e. D16S539, D78820 and D138317 are 0.9568, 0.9132 and 0.7427
respectively. Power of Discrimination (PD) for these three loci are 0.9135 (D16S539),
0.9331 (D78820) and 0.9050 (D13S317); PowerofExclusion (PE) was found to be 0.5192
(0168539), 0.6034 (078820) and 0.4053 (D13S317) respectively. The Cumulative
Discrimination Power (CDP) for the three 8TRs for Temiar population is 0.99903. |
| first_indexed | 2024-03-06T15:49:28Z |
| format | Monograph |
| id | usm.eprints-51130 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T15:49:28Z |
| publisher | Universiti Sains Malaysia |
| record_format | dspace |
| spelling | usm.eprints-511302022-01-11T07:35:26Z http://eprints.usm.my/51130/ Distribution of allele frequencies of short tandem repeats (STRs) DI6S539, D7S820 and D13S317 in random temiar population group of Malaysia Khor Kok, Pin R Medicine (General) Polymerase Chain Reaction based Short Tandem Repeat (PCR-STR) method provides an unprecedented DNA profiling technique for forensic community in identification and individualization purposes. In the analysis of biological material using DNA profiling technique, the information regarding the distribution of allele frequency in a defmed population is mandatory. This particularly necessary in paternity testing that involves compilation of Paternity Index (PI), in which the allele frequency is required to indicate whether the alleged father is the true biological father of the child. Validated 8TR loci have been analysed extensively in various populations for forensic application. In this research study, population genetic studies were carried out for three 8TR loci, namely D168539, D7S820 and D13S317 for Temiar population by using a multiplex PCR reaction followed by 4% polyacrylamide gel electrophoresis (PAGE) and silver staining. The heterozygosity (H) for these three loci, i.e. D16S539, D78820 and D138317 are 0.9568, 0.9132 and 0.7427 respectively. Power of Discrimination (PD) for these three loci are 0.9135 (D16S539), 0.9331 (D78820) and 0.9050 (D13S317); PowerofExclusion (PE) was found to be 0.5192 (0168539), 0.6034 (078820) and 0.4053 (D13S317) respectively. The Cumulative Discrimination Power (CDP) for the three 8TRs for Temiar population is 0.99903. Universiti Sains Malaysia Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/51130/1/KHOR%20POK%20PIN%20-%2024%20pages.pdf Khor Kok, Pin Distribution of allele frequencies of short tandem repeats (STRs) DI6S539, D7S820 and D13S317 in random temiar population group of Malaysia. Other. Universiti Sains Malaysia. (Submitted) |
| spellingShingle | R Medicine (General) Khor Kok, Pin Distribution of allele frequencies of short tandem repeats (STRs) DI6S539, D7S820 and D13S317 in random temiar population group of Malaysia |
| title | Distribution of allele frequencies of short
tandem repeats (STRs) DI6S539, D7S820 and D13S317 in random temiar population group of Malaysia |
| title_full | Distribution of allele frequencies of short
tandem repeats (STRs) DI6S539, D7S820 and D13S317 in random temiar population group of Malaysia |
| title_fullStr | Distribution of allele frequencies of short
tandem repeats (STRs) DI6S539, D7S820 and D13S317 in random temiar population group of Malaysia |
| title_full_unstemmed | Distribution of allele frequencies of short
tandem repeats (STRs) DI6S539, D7S820 and D13S317 in random temiar population group of Malaysia |
| title_short | Distribution of allele frequencies of short
tandem repeats (STRs) DI6S539, D7S820 and D13S317 in random temiar population group of Malaysia |
| title_sort | distribution of allele frequencies of short tandem repeats strs di6s539 d7s820 and d13s317 in random temiar population group of malaysia |
| topic | R Medicine (General) |
| url | http://eprints.usm.my/51130/1/KHOR%20POK%20PIN%20-%2024%20pages.pdf |
| work_keys_str_mv | AT khorkokpin distributionofallelefrequenciesofshorttandemrepeatsstrsdi6s539d7s820andd13s317inrandomtemiarpopulationgroupofmalaysia |