Effect of centella asiatica against gram negative bacteria
Centella asiatica (C. asiatica) is a well-known herb used in traditional medicine for treatment of a wide range of diseases. In this research, crude extract from different parts of C. asiatica extracted with two different solvents; methanol and water, were assessed for antibacterial activity agai...
| Main Author: | |
|---|---|
| Format: | Monograph |
| Language: | English |
| Published: |
Universiti Sains Malaysia
|
| Subjects: | |
| Online Access: | http://eprints.usm.my/51206/1/MIMI%20AZUANA%20BINTI%20WAGIMON%20-%2024%20pages.pdf |
| _version_ | 1825906443754143744 |
|---|---|
| author | Mimi Azuana, Wagimon |
| author_facet | Mimi Azuana, Wagimon |
| author_sort | Mimi Azuana, Wagimon |
| collection | USM |
| description | Centella asiatica (C. asiatica) is a well-known herb used in traditional medicine for
treatment of a wide range of diseases. In this research, crude extract from different parts of
C. asiatica extracted with two different solvents; methanol and water, were assessed for
antibacterial activity against Gram negative bacteria, Helicobacter pylori (H pylori) and
Escherichia coli (E. coli). The determination of minimum inhibitory concentration (MIC)
was performed using microbroth dilution method. The MICs values for H pylori were 0.04
mg/ml and 0.16 mg/ml for methanolic and water leaves crude extracts, respectively, 0.16
mg/ml and 0.04 mg/ml for methanolic and water stem extracts, respectively and 0.04 mg/ml
for both methanolic and water root extracts. The MICs values for E. coli were 0.01 mg/ml
and 0.02 mg/ml for methanolic and water leaves extracts, 0.01 mg/ml and 1.25 mg/ml for
methanolic and water stem extracts while 0.63 mg/ml and 1.25 mg/ml for methanolic and
water root extract respectively. The MICs values for positive controls, clarithromycin and
ampicillin, were 0.16 mg/ml and 0.01 mg/ml, respectively. Generally, all the C. asiatica
extracts had a promising antibacterial effect against these bacteria. |
| first_indexed | 2024-03-06T15:49:42Z |
| format | Monograph |
| id | usm.eprints-51206 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T15:49:42Z |
| publisher | Universiti Sains Malaysia |
| record_format | dspace |
| spelling | usm.eprints-512062022-01-16T04:42:07Z http://eprints.usm.my/51206/ Effect of centella asiatica against gram negative bacteria Mimi Azuana, Wagimon R Medicine (General) Centella asiatica (C. asiatica) is a well-known herb used in traditional medicine for treatment of a wide range of diseases. In this research, crude extract from different parts of C. asiatica extracted with two different solvents; methanol and water, were assessed for antibacterial activity against Gram negative bacteria, Helicobacter pylori (H pylori) and Escherichia coli (E. coli). The determination of minimum inhibitory concentration (MIC) was performed using microbroth dilution method. The MICs values for H pylori were 0.04 mg/ml and 0.16 mg/ml for methanolic and water leaves crude extracts, respectively, 0.16 mg/ml and 0.04 mg/ml for methanolic and water stem extracts, respectively and 0.04 mg/ml for both methanolic and water root extracts. The MICs values for E. coli were 0.01 mg/ml and 0.02 mg/ml for methanolic and water leaves extracts, 0.01 mg/ml and 1.25 mg/ml for methanolic and water stem extracts while 0.63 mg/ml and 1.25 mg/ml for methanolic and water root extract respectively. The MICs values for positive controls, clarithromycin and ampicillin, were 0.16 mg/ml and 0.01 mg/ml, respectively. Generally, all the C. asiatica extracts had a promising antibacterial effect against these bacteria. Universiti Sains Malaysia Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/51206/1/MIMI%20AZUANA%20BINTI%20WAGIMON%20-%2024%20pages.pdf Mimi Azuana, Wagimon Effect of centella asiatica against gram negative bacteria. Other. Universiti Sains Malaysia. (Submitted) |
| spellingShingle | R Medicine (General) Mimi Azuana, Wagimon Effect of centella asiatica against gram negative bacteria |
| title | Effect of centella asiatica against
gram negative bacteria |
| title_full | Effect of centella asiatica against
gram negative bacteria |
| title_fullStr | Effect of centella asiatica against
gram negative bacteria |
| title_full_unstemmed | Effect of centella asiatica against
gram negative bacteria |
| title_short | Effect of centella asiatica against
gram negative bacteria |
| title_sort | effect of centella asiatica against gram negative bacteria |
| topic | R Medicine (General) |
| url | http://eprints.usm.my/51206/1/MIMI%20AZUANA%20BINTI%20WAGIMON%20-%2024%20pages.pdf |
| work_keys_str_mv | AT mimiazuanawagimon effectofcentellaasiaticaagainstgramnegativebacteria |