Combinatorial Crispr/Cas9 For Suppression Of Latent Hiv-1 Provirus In Vitro
Although HAART effectively suppresses HIV-1 replication, its compromised effectiveness against non-B subtypes, the challenge of eliminating latent proviruses, life-long treatment, and viral resistance complicates the cure for HIV-1. CRISPR/Cas9, the latest genome editing tool, can overcome the limit...
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| Format: | Thesis |
| Language: | English |
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2020
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| Online Access: | http://eprints.usm.my/52124/1/Pages%20from%20Combinatorial%20CrisprCas9%20ForSuppression%20Of%20Latent%20Hiv-1%20Provirus%20In%20Vitro.pdf |
| _version_ | 1825906623187517440 |
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| author | Ravichantar, Nithya |
| author_facet | Ravichantar, Nithya |
| author_sort | Ravichantar, Nithya |
| collection | USM |
| description | Although HAART effectively suppresses HIV-1 replication, its compromised effectiveness against non-B subtypes, the challenge of eliminating latent proviruses, life-long treatment, and viral resistance complicates the cure for HIV-1. CRISPR/Cas9, the latest genome editing tool, can overcome the limitations seen with HAART. By targeting the promoter of HIV-1 (LTR), CRISPR/Cas9 can disrupt the latent reservoirs. Here, we designed an improved CRISPR/Cas9 system, combinatorial CRISPR/Cas9 to concurrently knockdown multiple HIV-1 genes: structural (Pol and Gag), regulatory (Rev and Tat) and accessory genes (Vif). We investigated the efficacy of this tool as a therapy against different viral subtypes in a subset population . Our preliminary data, showed a huge decrease in viral load and we verified the safety of CRISPR/Cas9 in human cell lines. |
| first_indexed | 2024-03-06T15:52:19Z |
| format | Thesis |
| id | usm.eprints-52124 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T15:52:19Z |
| publishDate | 2020 |
| record_format | dspace |
| spelling | usm.eprints-521242022-03-31T07:08:35Z http://eprints.usm.my/52124/ Combinatorial Crispr/Cas9 For Suppression Of Latent Hiv-1 Provirus In Vitro Ravichantar, Nithya R5-920 Medicine (General) Although HAART effectively suppresses HIV-1 replication, its compromised effectiveness against non-B subtypes, the challenge of eliminating latent proviruses, life-long treatment, and viral resistance complicates the cure for HIV-1. CRISPR/Cas9, the latest genome editing tool, can overcome the limitations seen with HAART. By targeting the promoter of HIV-1 (LTR), CRISPR/Cas9 can disrupt the latent reservoirs. Here, we designed an improved CRISPR/Cas9 system, combinatorial CRISPR/Cas9 to concurrently knockdown multiple HIV-1 genes: structural (Pol and Gag), regulatory (Rev and Tat) and accessory genes (Vif). We investigated the efficacy of this tool as a therapy against different viral subtypes in a subset population . Our preliminary data, showed a huge decrease in viral load and we verified the safety of CRISPR/Cas9 in human cell lines. 2020-11 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/52124/1/Pages%20from%20Combinatorial%20CrisprCas9%20ForSuppression%20Of%20Latent%20Hiv-1%20Provirus%20In%20Vitro.pdf Ravichantar, Nithya (2020) Combinatorial Crispr/Cas9 For Suppression Of Latent Hiv-1 Provirus In Vitro. PhD thesis, Universiti Sains Malaysia. |
| spellingShingle | R5-920 Medicine (General) Ravichantar, Nithya Combinatorial Crispr/Cas9 For Suppression Of Latent Hiv-1 Provirus In Vitro |
| title | Combinatorial Crispr/Cas9 For Suppression Of Latent Hiv-1 Provirus In Vitro |
| title_full | Combinatorial Crispr/Cas9 For Suppression Of Latent Hiv-1 Provirus In Vitro |
| title_fullStr | Combinatorial Crispr/Cas9 For Suppression Of Latent Hiv-1 Provirus In Vitro |
| title_full_unstemmed | Combinatorial Crispr/Cas9 For Suppression Of Latent Hiv-1 Provirus In Vitro |
| title_short | Combinatorial Crispr/Cas9 For Suppression Of Latent Hiv-1 Provirus In Vitro |
| title_sort | combinatorial crispr cas9 for suppression of latent hiv 1 provirus in vitro |
| topic | R5-920 Medicine (General) |
| url | http://eprints.usm.my/52124/1/Pages%20from%20Combinatorial%20CrisprCas9%20ForSuppression%20Of%20Latent%20Hiv-1%20Provirus%20In%20Vitro.pdf |
| work_keys_str_mv | AT ravichantarnithya combinatorialcrisprcas9forsuppressionoflatenthiv1provirusinvitro |