cDNA Clones Construction Of Dengue Virus Type-2 (New Guinea C-Strain)
Dengue virus type-2 (DEN-2) is a member of the Flaviviridae family. DEN-2 has a single-stranded positive polarity ribonucleic acid (RNA) genome of 10,723 nucleotides in length that encodes for a single polyprotein precursor. In this work, viral RNA genome was extracted from Vero and C6/36 cells in...
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| Format: | Thesis |
| Language: | English |
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2009
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| Online Access: | http://eprints.usm.my/52235/1/tesis%20DNA%20Clones%20cut.pdf |
| _version_ | 1797012776363753472 |
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| author | Ong, Eng Keat |
| author_facet | Ong, Eng Keat |
| author_sort | Ong, Eng Keat |
| collection | USM |
| description | Dengue virus type-2 (DEN-2) is a member of the Flaviviridae family. DEN-2 has a single-stranded positive polarity ribonucleic acid (RNA) genome of
10,723 nucleotides in length that encodes for a single polyprotein precursor. In this
work, viral RNA genome was extracted from Vero and C6/36 cells infected with
DEN-2 New Guinea C strain. The viral RNA genome of DEN-2 was reverse
transcribed and amplified as seven overlapping fragments, covering the whole
genome, by reverse transcription-polymerase chain reaction (RT-PCR). Each of
these fragments was successfully cloned into a high copy number plasmid, pGEMR-
T vector and transformed into Escherichia coli (E. coli) JM109 strain. The identity
of each clone was verified using DNA sequencing. The seven overlapping fragments
were successfully joined together in pGEMR-T vector and transformed into E. coli
JM109 strain at 25°C with the presence of ampicillin (25 pg/ml) to produce a cDNA
clone carrying the full-length genome of DEN-2. |
| first_indexed | 2024-03-06T15:52:38Z |
| format | Thesis |
| id | usm.eprints-52235 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T15:52:38Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | usm.eprints-522352022-04-07T08:06:08Z http://eprints.usm.my/52235/ cDNA Clones Construction Of Dengue Virus Type-2 (New Guinea C-Strain) Ong, Eng Keat QH1 Natural history (General - Including nature conservation, geographical distribution) Dengue virus type-2 (DEN-2) is a member of the Flaviviridae family. DEN-2 has a single-stranded positive polarity ribonucleic acid (RNA) genome of 10,723 nucleotides in length that encodes for a single polyprotein precursor. In this work, viral RNA genome was extracted from Vero and C6/36 cells infected with DEN-2 New Guinea C strain. The viral RNA genome of DEN-2 was reverse transcribed and amplified as seven overlapping fragments, covering the whole genome, by reverse transcription-polymerase chain reaction (RT-PCR). Each of these fragments was successfully cloned into a high copy number plasmid, pGEMR- T vector and transformed into Escherichia coli (E. coli) JM109 strain. The identity of each clone was verified using DNA sequencing. The seven overlapping fragments were successfully joined together in pGEMR-T vector and transformed into E. coli JM109 strain at 25°C with the presence of ampicillin (25 pg/ml) to produce a cDNA clone carrying the full-length genome of DEN-2. 2009-04 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/52235/1/tesis%20DNA%20Clones%20cut.pdf Ong, Eng Keat (2009) cDNA Clones Construction Of Dengue Virus Type-2 (New Guinea C-Strain). Masters thesis, Universiti Sains Malaysia. |
| spellingShingle | QH1 Natural history (General - Including nature conservation, geographical distribution) Ong, Eng Keat cDNA Clones Construction Of Dengue Virus Type-2 (New Guinea C-Strain) |
| title | cDNA Clones Construction Of Dengue Virus Type-2
(New Guinea C-Strain) |
| title_full | cDNA Clones Construction Of Dengue Virus Type-2
(New Guinea C-Strain) |
| title_fullStr | cDNA Clones Construction Of Dengue Virus Type-2
(New Guinea C-Strain) |
| title_full_unstemmed | cDNA Clones Construction Of Dengue Virus Type-2
(New Guinea C-Strain) |
| title_short | cDNA Clones Construction Of Dengue Virus Type-2
(New Guinea C-Strain) |
| title_sort | cdna clones construction of dengue virus type 2 new guinea c strain |
| topic | QH1 Natural history (General - Including nature conservation, geographical distribution) |
| url | http://eprints.usm.my/52235/1/tesis%20DNA%20Clones%20cut.pdf |
| work_keys_str_mv | AT ongengkeat cdnaclonesconstructionofdenguevirustype2newguineacstrain |