Deposition Of Nylon Membrane On Carbon Electrode For The Development Of Ascorbic Acid Electrochemical Biosensor

Electrochemical biosensor is the combination of electroanalytical methods of high sensitivity with a presence of biorecognition system that helps in identifying target species. The main objective of this work is to study the conjugation of ascorbic acid (AA) to polyaniline (PANI) and to explore th...

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Main Author: Harith, Noor Nadhirah
Format: Monograph
Language:English
Published: Universiti Sains Malaysia 2018
Subjects:
Online Access:http://eprints.usm.my/53672/1/Deposition%20Of%20Nylon%20Membrane%20On%20Carbon%20Electrode%20For%20The%20Development%20Of%20Ascorbic%20Acid%20Electrochemical%20Biosensor_Noor%20Nadhirah%20Harith_K4_2018.pdf
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author Harith, Noor Nadhirah
author_facet Harith, Noor Nadhirah
author_sort Harith, Noor Nadhirah
collection USM
description Electrochemical biosensor is the combination of electroanalytical methods of high sensitivity with a presence of biorecognition system that helps in identifying target species. The main objective of this work is to study the conjugation of ascorbic acid (AA) to polyaniline (PANI) and to explore the cyclic voltammetry and electrochemical impedance spectroscopy of the membrane-based sensor to detect AA. In this study, the conjugation of ascorbic AA was first done by the synthesis of PANI/Fe2O3, before the AA was interacted with PANI/Fe2O3. UV-Vis spectrometer absorbance for solutions PBS, AA of 50 mM, PANI, PANI/Fe2O3 and PANI/Fe2O3/AA of 50 mM were measured to prove the successful conjugation of AA. Next, membrane-sensor was assembled, bare glass carbon electrode (GCE), GCE-nylon membrane and GCE-nylon membrane immobilized with ascorbate oxidase were testing with AA. The cyclic voltammetry pattern and electrochemical impedance spectroscopy were recorded to study the redox potential and conductivity of the testing solutions.
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spelling usm.eprints-536722022-07-28T03:22:25Z http://eprints.usm.my/53672/ Deposition Of Nylon Membrane On Carbon Electrode For The Development Of Ascorbic Acid Electrochemical Biosensor Harith, Noor Nadhirah T Technology TP Chemical Technology Electrochemical biosensor is the combination of electroanalytical methods of high sensitivity with a presence of biorecognition system that helps in identifying target species. The main objective of this work is to study the conjugation of ascorbic acid (AA) to polyaniline (PANI) and to explore the cyclic voltammetry and electrochemical impedance spectroscopy of the membrane-based sensor to detect AA. In this study, the conjugation of ascorbic AA was first done by the synthesis of PANI/Fe2O3, before the AA was interacted with PANI/Fe2O3. UV-Vis spectrometer absorbance for solutions PBS, AA of 50 mM, PANI, PANI/Fe2O3 and PANI/Fe2O3/AA of 50 mM were measured to prove the successful conjugation of AA. Next, membrane-sensor was assembled, bare glass carbon electrode (GCE), GCE-nylon membrane and GCE-nylon membrane immobilized with ascorbate oxidase were testing with AA. The cyclic voltammetry pattern and electrochemical impedance spectroscopy were recorded to study the redox potential and conductivity of the testing solutions. Universiti Sains Malaysia 2018-06-01 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/53672/1/Deposition%20Of%20Nylon%20Membrane%20On%20Carbon%20Electrode%20For%20The%20Development%20Of%20Ascorbic%20Acid%20Electrochemical%20Biosensor_Noor%20Nadhirah%20Harith_K4_2018.pdf Harith, Noor Nadhirah (2018) Deposition Of Nylon Membrane On Carbon Electrode For The Development Of Ascorbic Acid Electrochemical Biosensor. Project Report. Universiti Sains Malaysia, Pusat Pengajian Kejuruteraan Kimia. (Submitted)
spellingShingle T Technology
TP Chemical Technology
Harith, Noor Nadhirah
Deposition Of Nylon Membrane On Carbon Electrode For The Development Of Ascorbic Acid Electrochemical Biosensor
title Deposition Of Nylon Membrane On Carbon Electrode For The Development Of Ascorbic Acid Electrochemical Biosensor
title_full Deposition Of Nylon Membrane On Carbon Electrode For The Development Of Ascorbic Acid Electrochemical Biosensor
title_fullStr Deposition Of Nylon Membrane On Carbon Electrode For The Development Of Ascorbic Acid Electrochemical Biosensor
title_full_unstemmed Deposition Of Nylon Membrane On Carbon Electrode For The Development Of Ascorbic Acid Electrochemical Biosensor
title_short Deposition Of Nylon Membrane On Carbon Electrode For The Development Of Ascorbic Acid Electrochemical Biosensor
title_sort deposition of nylon membrane on carbon electrode for the development of ascorbic acid electrochemical biosensor
topic T Technology
TP Chemical Technology
url http://eprints.usm.my/53672/1/Deposition%20Of%20Nylon%20Membrane%20On%20Carbon%20Electrode%20For%20The%20Development%20Of%20Ascorbic%20Acid%20Electrochemical%20Biosensor_Noor%20Nadhirah%20Harith_K4_2018.pdf
work_keys_str_mv AT harithnoornadhirah depositionofnylonmembraneoncarbonelectrodeforthedevelopmentofascorbicacidelectrochemicalbiosensor