Esterification Of Ethanol And Acetic Acid Catalysed By Immobilized Candida Rugosa Lipase
Ethyl acetate is a versatile ester used as solvent and diluents. The conventional esterification reaction of ethanol and acetic acid was catalysed by sulphuric acid, H2SO4. Enzyme is a sustainable approach for the esterification reaction to produce ethyl acetate. In this study, the esterification...
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| Format: | Monograph |
| Language: | English |
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Universiti Sains Malaysia
2021
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| Online Access: | http://eprints.usm.my/54734/1/Esterification%20Of%20Ethanol%20And%20Acetic%20Acid%20Catalysed%20By%20Immobilized%20Candida%20Rugosa%20Lipase_Nurul%20Adila%20Mohd%20Radzi_K4_2021_ESAR.pdf |
| _version_ | 1797013283747659776 |
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| author | Mohd Radzi, Nurul Adila |
| author_facet | Mohd Radzi, Nurul Adila |
| author_sort | Mohd Radzi, Nurul Adila |
| collection | USM |
| description | Ethyl acetate is a versatile ester used as solvent and diluents. The conventional esterification reaction of ethanol and acetic acid was catalysed by sulphuric acid, H2SO4.
Enzyme is a sustainable approach for the esterification reaction to produce ethyl acetate. In this study, the esterification reaction of ethanol and acetic acid catalysed by immobilized candida rugosa lipase (CRL) in n-hexane was optimized. The free CRL was immobilized by physical
adsorption on support, Amberlite XAD7 which resulted in specific activity of 0.13 U/mg. The effect of reaction time, temperature, substrate molar ratio and enzyme loading were studied. Maximum conversion of 88% was attained at 2 hours of reaction time, temperature of 50°C, acetic acid to ethanol ratio of 0.5 and enzyme loading of 80 U. Next, the kinetic modelling of bi-substrate enzymatic transesterification of ethyl butyrate was studied using secondary data from research paper. The data was fitted to the rate equation of the kinetic model using non-linear regression to obtain the kinetic parameter. Based on the Lineweaver-Burk double reciprocal plot, the transesterification reaction follows ping-pong bi-bi mechanism with competitive inhibition by ethyl caprate. The kinetic parameters obtained was, Vmax = 1.1918 M, Km,A = 0.0117 M, Km,B = 0.1674, Ki,A = 0.1091 M, and Ki,B = 0.0031. |
| first_indexed | 2024-03-06T15:59:57Z |
| format | Monograph |
| id | usm.eprints-54734 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T15:59:57Z |
| publishDate | 2021 |
| publisher | Universiti Sains Malaysia |
| record_format | dspace |
| spelling | usm.eprints-547342022-09-15T08:16:57Z http://eprints.usm.my/54734/ Esterification Of Ethanol And Acetic Acid Catalysed By Immobilized Candida Rugosa Lipase Mohd Radzi, Nurul Adila T Technology TP Chemical Technology Ethyl acetate is a versatile ester used as solvent and diluents. The conventional esterification reaction of ethanol and acetic acid was catalysed by sulphuric acid, H2SO4. Enzyme is a sustainable approach for the esterification reaction to produce ethyl acetate. In this study, the esterification reaction of ethanol and acetic acid catalysed by immobilized candida rugosa lipase (CRL) in n-hexane was optimized. The free CRL was immobilized by physical adsorption on support, Amberlite XAD7 which resulted in specific activity of 0.13 U/mg. The effect of reaction time, temperature, substrate molar ratio and enzyme loading were studied. Maximum conversion of 88% was attained at 2 hours of reaction time, temperature of 50°C, acetic acid to ethanol ratio of 0.5 and enzyme loading of 80 U. Next, the kinetic modelling of bi-substrate enzymatic transesterification of ethyl butyrate was studied using secondary data from research paper. The data was fitted to the rate equation of the kinetic model using non-linear regression to obtain the kinetic parameter. Based on the Lineweaver-Burk double reciprocal plot, the transesterification reaction follows ping-pong bi-bi mechanism with competitive inhibition by ethyl caprate. The kinetic parameters obtained was, Vmax = 1.1918 M, Km,A = 0.0117 M, Km,B = 0.1674, Ki,A = 0.1091 M, and Ki,B = 0.0031. Universiti Sains Malaysia 2021-06-01 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/54734/1/Esterification%20Of%20Ethanol%20And%20Acetic%20Acid%20Catalysed%20By%20Immobilized%20Candida%20Rugosa%20Lipase_Nurul%20Adila%20Mohd%20Radzi_K4_2021_ESAR.pdf Mohd Radzi, Nurul Adila (2021) Esterification Of Ethanol And Acetic Acid Catalysed By Immobilized Candida Rugosa Lipase. Project Report. Universiti Sains Malaysia, Pusat Pengajian Kejuruteraan Kimia. (Submitted) |
| spellingShingle | T Technology TP Chemical Technology Mohd Radzi, Nurul Adila Esterification Of Ethanol And Acetic Acid Catalysed By Immobilized Candida Rugosa Lipase |
| title | Esterification Of Ethanol And Acetic Acid Catalysed By Immobilized Candida Rugosa Lipase |
| title_full | Esterification Of Ethanol And Acetic Acid Catalysed By Immobilized Candida Rugosa Lipase |
| title_fullStr | Esterification Of Ethanol And Acetic Acid Catalysed By Immobilized Candida Rugosa Lipase |
| title_full_unstemmed | Esterification Of Ethanol And Acetic Acid Catalysed By Immobilized Candida Rugosa Lipase |
| title_short | Esterification Of Ethanol And Acetic Acid Catalysed By Immobilized Candida Rugosa Lipase |
| title_sort | esterification of ethanol and acetic acid catalysed by immobilized candida rugosa lipase |
| topic | T Technology TP Chemical Technology |
| url | http://eprints.usm.my/54734/1/Esterification%20Of%20Ethanol%20And%20Acetic%20Acid%20Catalysed%20By%20Immobilized%20Candida%20Rugosa%20Lipase_Nurul%20Adila%20Mohd%20Radzi_K4_2021_ESAR.pdf |
| work_keys_str_mv | AT mohdradzinuruladila esterificationofethanolandaceticacidcatalysedbyimmobilizedcandidarugosalipase |