Detection of apoptosis in human macrophage cell line, THP-1 infected with BCG and recombinant BCG (rBCG) expressing the 19-kDa C-terminus of the Merozoite Surface Protein-1 (MSP-1C)
Malaria is a major cause of disease and death in tropical countries. With the spread of insecticide-resistant mosquito vector and increase in the prevalence of resistant parasite to anti-malarial drugs, there is an urgent need to develop an effective vaccine to control malaria infection. By utiliz...
| Main Author: | |
|---|---|
| Format: | Monograph |
| Language: | English |
| Published: |
Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia
2013
|
| Subjects: | |
| Online Access: | http://eprints.usm.my/56953/1/CHEONG%20SIEW%20MEI-24%20pages.pdf |
| _version_ | 1825907596580618240 |
|---|---|
| author | Mei, Cheong Siew |
| author_facet | Mei, Cheong Siew |
| author_sort | Mei, Cheong Siew |
| collection | USM |
| description | Malaria is a major cause of disease and death in tropical countries. With the spread of
insecticide-resistant mosquito vector and increase in the prevalence of resistant parasite to
anti-malarial drugs, there is an urgent need to develop an effective vaccine to control malaria infection. By utilizing Mycobacterium bovis BCG as a delivery system, a
recombinant BCG vaccine expressing the 19 kDa MSP-1C of P. falciparum was
constructed previously by assembly PCR. This vaccine candidate has been known to be
capable of stimulating humoral immune response in mice and induce activation of mouse
macrophage cell line in vitro. However, the effect of the rBCG vaccine on macrophage
apoptosis, an important mechanism for parasite clearance has not been determined. Thus,
the objective of this study was to determine the apoptosis activity of human macrophage
cell line THP-1 infected with the rBCG clone. The apoptosis of untreated macrophage,
macrophage infected with BCG and macrophage stimulated with LPS were used as
controls. Expression of caspase 9 was also determined in order to identify the pathway
involved in the apoptosis activity. The results demonstrated that macrophage apoptosis
would be detected in all macrophage culture conditions. However, the apoptosis activity
macrophages and LPS-stimulated macrophages. In addition, ELISA analysis also showed
that caspase 9 activity was increased in rBCG infected macrophages compare to those of
BCG and LPS stimulated macrophages. In conclusion, these data demonstrated that the
presence of MSP-1C in rBCG increased macrophage apoptotic activity and the expression
of caspase 9 in the infected macrophages. Thus, the rBCG candidate vaccine is potential to
be used to control malaria infection in the future |
| first_indexed | 2024-03-06T16:06:33Z |
| format | Monograph |
| id | usm.eprints-56953 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T16:06:33Z |
| publishDate | 2013 |
| publisher | Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia |
| record_format | dspace |
| spelling | usm.eprints-569532023-03-01T04:31:13Z http://eprints.usm.my/56953/ Detection of apoptosis in human macrophage cell line, THP-1 infected with BCG and recombinant BCG (rBCG) expressing the 19-kDa C-terminus of the Merozoite Surface Protein-1 (MSP-1C) Mei, Cheong Siew R Medicine RA643-645 Disease (Communicable and noninfectious) and public health Malaria is a major cause of disease and death in tropical countries. With the spread of insecticide-resistant mosquito vector and increase in the prevalence of resistant parasite to anti-malarial drugs, there is an urgent need to develop an effective vaccine to control malaria infection. By utilizing Mycobacterium bovis BCG as a delivery system, a recombinant BCG vaccine expressing the 19 kDa MSP-1C of P. falciparum was constructed previously by assembly PCR. This vaccine candidate has been known to be capable of stimulating humoral immune response in mice and induce activation of mouse macrophage cell line in vitro. However, the effect of the rBCG vaccine on macrophage apoptosis, an important mechanism for parasite clearance has not been determined. Thus, the objective of this study was to determine the apoptosis activity of human macrophage cell line THP-1 infected with the rBCG clone. The apoptosis of untreated macrophage, macrophage infected with BCG and macrophage stimulated with LPS were used as controls. Expression of caspase 9 was also determined in order to identify the pathway involved in the apoptosis activity. The results demonstrated that macrophage apoptosis would be detected in all macrophage culture conditions. However, the apoptosis activity macrophages and LPS-stimulated macrophages. In addition, ELISA analysis also showed that caspase 9 activity was increased in rBCG infected macrophages compare to those of BCG and LPS stimulated macrophages. In conclusion, these data demonstrated that the presence of MSP-1C in rBCG increased macrophage apoptotic activity and the expression of caspase 9 in the infected macrophages. Thus, the rBCG candidate vaccine is potential to be used to control malaria infection in the future Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia 2013-05 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/56953/1/CHEONG%20SIEW%20MEI-24%20pages.pdf Mei, Cheong Siew (2013) Detection of apoptosis in human macrophage cell line, THP-1 infected with BCG and recombinant BCG (rBCG) expressing the 19-kDa C-terminus of the Merozoite Surface Protein-1 (MSP-1C). Other. Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia. (Submitted) |
| spellingShingle | R Medicine RA643-645 Disease (Communicable and noninfectious) and public health Mei, Cheong Siew Detection of apoptosis in human macrophage cell line, THP-1 infected with BCG and recombinant BCG (rBCG) expressing the 19-kDa C-terminus of the Merozoite Surface Protein-1 (MSP-1C) |
| title | Detection of apoptosis in human macrophage cell line, THP-1 infected with BCG and recombinant BCG (rBCG) expressing the 19-kDa C-terminus of the Merozoite Surface Protein-1 (MSP-1C) |
| title_full | Detection of apoptosis in human macrophage cell line, THP-1 infected with BCG and recombinant BCG (rBCG) expressing the 19-kDa C-terminus of the Merozoite Surface Protein-1 (MSP-1C) |
| title_fullStr | Detection of apoptosis in human macrophage cell line, THP-1 infected with BCG and recombinant BCG (rBCG) expressing the 19-kDa C-terminus of the Merozoite Surface Protein-1 (MSP-1C) |
| title_full_unstemmed | Detection of apoptosis in human macrophage cell line, THP-1 infected with BCG and recombinant BCG (rBCG) expressing the 19-kDa C-terminus of the Merozoite Surface Protein-1 (MSP-1C) |
| title_short | Detection of apoptosis in human macrophage cell line, THP-1 infected with BCG and recombinant BCG (rBCG) expressing the 19-kDa C-terminus of the Merozoite Surface Protein-1 (MSP-1C) |
| title_sort | detection of apoptosis in human macrophage cell line thp 1 infected with bcg and recombinant bcg rbcg expressing the 19 kda c terminus of the merozoite surface protein 1 msp 1c |
| topic | R Medicine RA643-645 Disease (Communicable and noninfectious) and public health |
| url | http://eprints.usm.my/56953/1/CHEONG%20SIEW%20MEI-24%20pages.pdf |
| work_keys_str_mv | AT meicheongsiew detectionofapoptosisinhumanmacrophagecelllinethp1infectedwithbcgandrecombinantbcgrbcgexpressingthe19kdacterminusofthemerozoitesurfaceprotein1msp1c |