Anticancer effect of artonin E and chaplashin from artocarpus species on breast cancer cells, MDA-MB-231 and MCF-7
Breast cancer appears to be a major global health problem. The most common treatment approach of this disease is chemotherapy. However, studies showed that long-term use lead to drug resistance and implies side effect that may cause several genetic alteration, such as gene mutation, DNA methylati...
| Main Author: | |
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| Format: | Monograph |
| Language: | English |
| Published: |
Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia
2013
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| Subjects: | |
| Online Access: | http://eprints.usm.my/56958/1/CHOO%20YEN%20LING-24%20pages.pdf |
| _version_ | 1797013756445720576 |
|---|---|
| author | Ling, Choo Yen |
| author_facet | Ling, Choo Yen |
| author_sort | Ling, Choo Yen |
| collection | USM |
| description | Breast cancer appears to be a major global health problem. The most common treatment
approach of this disease is chemotherapy. However, studies showed that long-term use
lead to drug resistance and implies side effect that may cause
several genetic alteration, such as gene mutation, DNA methylation and histone
modification. Epidemiologic studies revealed that consuming flavonoid rich fruits and
vegetables might reduce all types of cancer incidences including breast cancer.
Therefore, this research was conducted to evaluate the anticancer effect of two types of
flavonoid extracted from Artocarpus sp, Artonin E and Chaplashin towards breast
determine anticancer effect of Artonin E and Chaplashin towards MDA-MB-231 and
MCF-7 breast cancer cell lines and to determine mode of cell death of cells treated with
most potent compound by assessing nuclear fragmentation effect. Dose-dependent
inhibitory effect of cell growth on MDA-MB-231 and MCF-7 breast cancer cells with a
median inhibition concentration (IC50) was determined by methylene blue assay. The
IC50 concentration was then used to analyse nuclear fragmentation event using
fluorescence dye Hoeschst 33342. Mode of cell death was confirmed by flow
cytometry analysis using Annexin-V/PI double staining. It was found that Artonin E
showed better anticancer activity towards MDA-MB-231 cells with IC50 of 4.95 pg/ml
compared to Cisplatin with IC50 of 5.88 pg/ml. Chaplashin showed the best activity
towards MDA-MB-231 breast cancer cells with IC50 of 24.80 pg/ml. Nuclear
fragmentation assay showed the cells treated with Artonin E absorbed more distinct
fluorescent blue glow compared to the untreated cells. The stained morphology
indicated apoptosis characteristic as nuclear fragmentation appeared clearly in the
treated cells. Thus, we postulated that anticancer effect in the treated cells underwent
apoptosis. In conclusion, Artonin E exerted strong antipoliferative effects to attribute
anticancer effect potential towards MDA-MB-231. Mode of cell death confirmed the
induction of apoptosis when nuclear fragmentation occurred in the treated cancer cells.
Therefore, these plant-derived compounds need further investigation to support the
anticancer effect. Thus it might become a potential anticancer drug in the future. |
| first_indexed | 2024-03-06T16:06:34Z |
| format | Monograph |
| id | usm.eprints-56958 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T16:06:34Z |
| publishDate | 2013 |
| publisher | Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia |
| record_format | dspace |
| spelling | usm.eprints-569582023-09-19T01:38:16Z http://eprints.usm.my/56958/ Anticancer effect of artonin E and chaplashin from artocarpus species on breast cancer cells, MDA-MB-231 and MCF-7 Ling, Choo Yen R Medicine RC254-282 Neoplasms. Tumors. Oncology (including Cancer) Breast cancer appears to be a major global health problem. The most common treatment approach of this disease is chemotherapy. However, studies showed that long-term use lead to drug resistance and implies side effect that may cause several genetic alteration, such as gene mutation, DNA methylation and histone modification. Epidemiologic studies revealed that consuming flavonoid rich fruits and vegetables might reduce all types of cancer incidences including breast cancer. Therefore, this research was conducted to evaluate the anticancer effect of two types of flavonoid extracted from Artocarpus sp, Artonin E and Chaplashin towards breast determine anticancer effect of Artonin E and Chaplashin towards MDA-MB-231 and MCF-7 breast cancer cell lines and to determine mode of cell death of cells treated with most potent compound by assessing nuclear fragmentation effect. Dose-dependent inhibitory effect of cell growth on MDA-MB-231 and MCF-7 breast cancer cells with a median inhibition concentration (IC50) was determined by methylene blue assay. The IC50 concentration was then used to analyse nuclear fragmentation event using fluorescence dye Hoeschst 33342. Mode of cell death was confirmed by flow cytometry analysis using Annexin-V/PI double staining. It was found that Artonin E showed better anticancer activity towards MDA-MB-231 cells with IC50 of 4.95 pg/ml compared to Cisplatin with IC50 of 5.88 pg/ml. Chaplashin showed the best activity towards MDA-MB-231 breast cancer cells with IC50 of 24.80 pg/ml. Nuclear fragmentation assay showed the cells treated with Artonin E absorbed more distinct fluorescent blue glow compared to the untreated cells. The stained morphology indicated apoptosis characteristic as nuclear fragmentation appeared clearly in the treated cells. Thus, we postulated that anticancer effect in the treated cells underwent apoptosis. In conclusion, Artonin E exerted strong antipoliferative effects to attribute anticancer effect potential towards MDA-MB-231. Mode of cell death confirmed the induction of apoptosis when nuclear fragmentation occurred in the treated cancer cells. Therefore, these plant-derived compounds need further investigation to support the anticancer effect. Thus it might become a potential anticancer drug in the future. Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia 2013-05 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/56958/1/CHOO%20YEN%20LING-24%20pages.pdf Ling, Choo Yen (2013) Anticancer effect of artonin E and chaplashin from artocarpus species on breast cancer cells, MDA-MB-231 and MCF-7. Other. Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia. (Submitted) |
| spellingShingle | R Medicine RC254-282 Neoplasms. Tumors. Oncology (including Cancer) Ling, Choo Yen Anticancer effect of artonin E and chaplashin from artocarpus species on breast cancer cells, MDA-MB-231 and MCF-7 |
| title | Anticancer effect of artonin E and chaplashin from artocarpus species on breast cancer cells, MDA-MB-231 and MCF-7 |
| title_full | Anticancer effect of artonin E and chaplashin from artocarpus species on breast cancer cells, MDA-MB-231 and MCF-7 |
| title_fullStr | Anticancer effect of artonin E and chaplashin from artocarpus species on breast cancer cells, MDA-MB-231 and MCF-7 |
| title_full_unstemmed | Anticancer effect of artonin E and chaplashin from artocarpus species on breast cancer cells, MDA-MB-231 and MCF-7 |
| title_short | Anticancer effect of artonin E and chaplashin from artocarpus species on breast cancer cells, MDA-MB-231 and MCF-7 |
| title_sort | anticancer effect of artonin e and chaplashin from artocarpus species on breast cancer cells mda mb 231 and mcf 7 |
| topic | R Medicine RC254-282 Neoplasms. Tumors. Oncology (including Cancer) |
| url | http://eprints.usm.my/56958/1/CHOO%20YEN%20LING-24%20pages.pdf |
| work_keys_str_mv | AT lingchooyen anticancereffectofartonineandchaplashinfromartocarpusspeciesonbreastcancercellsmdamb231andmcf7 |