Malaysian propolis extract alleviates macrovascular complication in diabetic rats: pharmacological potential of natural bee product

Malaysian propolis (MP) derived from stingless bee: (Heterotrigona itama) possesses antihyperglycemic effect in vivo and show antioxidant activity. The aim of the study was to observe the histological changes in aorta of streptozotocin-induced diabetic rats following MP administration. Thirty adul...

Full description

Bibliographic Details
Main Author: Yeoh, Boon Seng
Format: Article
Language:English
Published: Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia 2018
Subjects:
Online Access:http://eprints.usm.my/56996/1/DR%20BOON%20SENG%20YEOH-Eprints.pdf
_version_ 1825907605260730368
author Yeoh, Boon Seng
author_facet Yeoh, Boon Seng
author_sort Yeoh, Boon Seng
collection USM
description Malaysian propolis (MP) derived from stingless bee: (Heterotrigona itama) possesses antihyperglycemic effect in vivo and show antioxidant activity. The aim of the study was to observe the histological changes in aorta of streptozotocin-induced diabetic rats following MP administration. Thirty adult male Sprague Dawley rats were randomly assigned into five groups (n=6/group) of normoglycemic control (A): diabetic (DM) control (8); DM with metformin (C); DM with MP (D); DM with metformin and MP combination (E). Intraperitoneal streptozotocin (60mg/kg body weight) was employed to induce diabetes mellitus. Distilled water (I ml/day) as vehicle (control). met form in (300mg/kg/day) and MP (300mg/kg/day) were administered orally for four weeks, immediately after successful diabetic induction. The arch of aorta was harvested for histological study using Hematoxylin & Eosin and Verhoeff-Van Gieson stains. Aorta cross sections were examined under light microscope for changes in tunica intima media. Tissues deposition in tunica intima marks the outset of accelerated atherosclerosis in diabetes mellitus. The mean (standard error) or intimal thickness was found to be 2.83 (0.21), 4.52(1.00), 2.68 (0.39), 2.54 (0.36) and 2.11 (0.35) µm for group A, B, C, D and E respectively. This study found a significant increase ( 1.6 fold) in tunica intima thickness in the DM group compared to the normoglycemic control (p<0.05 ). There was no significant difference in tunica media thickness and number of elastic lamellae. ln conclusion, we infer that supplementation with MP modifies early progression or diabetic macrovascular complication and defers adverse sequalae or diabetes mellitus on aorta. with additional mechanism beside its anti-hyperglycemic effect. warranting future research.
first_indexed 2024-03-06T16:06:41Z
format Article
id usm.eprints-56996
institution Universiti Sains Malaysia
language English
last_indexed 2024-03-06T16:06:41Z
publishDate 2018
publisher Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia
record_format dspace
spelling usm.eprints-569962023-03-01T07:46:47Z http://eprints.usm.my/56996/ Malaysian propolis extract alleviates macrovascular complication in diabetic rats: pharmacological potential of natural bee product Yeoh, Boon Seng R Medicine RM300-666 Drugs and their actions Malaysian propolis (MP) derived from stingless bee: (Heterotrigona itama) possesses antihyperglycemic effect in vivo and show antioxidant activity. The aim of the study was to observe the histological changes in aorta of streptozotocin-induced diabetic rats following MP administration. Thirty adult male Sprague Dawley rats were randomly assigned into five groups (n=6/group) of normoglycemic control (A): diabetic (DM) control (8); DM with metformin (C); DM with MP (D); DM with metformin and MP combination (E). Intraperitoneal streptozotocin (60mg/kg body weight) was employed to induce diabetes mellitus. Distilled water (I ml/day) as vehicle (control). met form in (300mg/kg/day) and MP (300mg/kg/day) were administered orally for four weeks, immediately after successful diabetic induction. The arch of aorta was harvested for histological study using Hematoxylin & Eosin and Verhoeff-Van Gieson stains. Aorta cross sections were examined under light microscope for changes in tunica intima media. Tissues deposition in tunica intima marks the outset of accelerated atherosclerosis in diabetes mellitus. The mean (standard error) or intimal thickness was found to be 2.83 (0.21), 4.52(1.00), 2.68 (0.39), 2.54 (0.36) and 2.11 (0.35) µm for group A, B, C, D and E respectively. This study found a significant increase ( 1.6 fold) in tunica intima thickness in the DM group compared to the normoglycemic control (p<0.05 ). There was no significant difference in tunica media thickness and number of elastic lamellae. ln conclusion, we infer that supplementation with MP modifies early progression or diabetic macrovascular complication and defers adverse sequalae or diabetes mellitus on aorta. with additional mechanism beside its anti-hyperglycemic effect. warranting future research. Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia 2018 Article NonPeerReviewed application/pdf en http://eprints.usm.my/56996/1/DR%20BOON%20SENG%20YEOH-Eprints.pdf Yeoh, Boon Seng (2018) Malaysian propolis extract alleviates macrovascular complication in diabetic rats: pharmacological potential of natural bee product. 34th International Conference on Natural Products 2018. (Submitted)
spellingShingle R Medicine
RM300-666 Drugs and their actions
Yeoh, Boon Seng
Malaysian propolis extract alleviates macrovascular complication in diabetic rats: pharmacological potential of natural bee product
title Malaysian propolis extract alleviates macrovascular complication in diabetic rats: pharmacological potential of natural bee product
title_full Malaysian propolis extract alleviates macrovascular complication in diabetic rats: pharmacological potential of natural bee product
title_fullStr Malaysian propolis extract alleviates macrovascular complication in diabetic rats: pharmacological potential of natural bee product
title_full_unstemmed Malaysian propolis extract alleviates macrovascular complication in diabetic rats: pharmacological potential of natural bee product
title_short Malaysian propolis extract alleviates macrovascular complication in diabetic rats: pharmacological potential of natural bee product
title_sort malaysian propolis extract alleviates macrovascular complication in diabetic rats pharmacological potential of natural bee product
topic R Medicine
RM300-666 Drugs and their actions
url http://eprints.usm.my/56996/1/DR%20BOON%20SENG%20YEOH-Eprints.pdf
work_keys_str_mv AT yeohboonseng malaysianpropolisextractalleviatesmacrovascularcomplicationindiabeticratspharmacologicalpotentialofnaturalbeeproduct