Malaysian propolis extract alleviates macrovascular complication in diabetic rats: pharmacological potential of natural bee product
Malaysian propolis (MP) derived from stingless bee: (Heterotrigona itama) possesses antihyperglycemic effect in vivo and show antioxidant activity. The aim of the study was to observe the histological changes in aorta of streptozotocin-induced diabetic rats following MP administration. Thirty adul...
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| Format: | Article |
| Language: | English |
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Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia
2018
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| Online Access: | http://eprints.usm.my/56996/1/DR%20BOON%20SENG%20YEOH-Eprints.pdf |
| _version_ | 1825907605260730368 |
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| author | Yeoh, Boon Seng |
| author_facet | Yeoh, Boon Seng |
| author_sort | Yeoh, Boon Seng |
| collection | USM |
| description | Malaysian propolis (MP) derived from stingless bee: (Heterotrigona itama) possesses antihyperglycemic effect in vivo and show antioxidant activity. The aim of the study was to observe
the histological changes in aorta of streptozotocin-induced diabetic rats following MP
administration. Thirty adult male Sprague Dawley rats were randomly assigned into five
groups (n=6/group) of normoglycemic control (A): diabetic (DM) control (8); DM with
metformin (C); DM with MP (D); DM with metformin and MP combination (E).
Intraperitoneal streptozotocin (60mg/kg body weight) was employed to induce diabetes
mellitus. Distilled water (I ml/day) as vehicle (control). met form in (300mg/kg/day) and MP
(300mg/kg/day) were administered orally for four weeks, immediately after successful diabetic
induction. The arch of aorta was harvested for histological study using Hematoxylin & Eosin
and Verhoeff-Van Gieson stains. Aorta cross sections were examined under light microscope
for changes in tunica intima media. Tissues deposition in tunica intima marks the outset of
accelerated atherosclerosis in diabetes mellitus. The mean (standard error) or intimal thickness
was found to be 2.83 (0.21), 4.52(1.00), 2.68 (0.39), 2.54 (0.36) and 2.11 (0.35) µm for group A, B, C, D and E respectively. This study found a significant increase ( 1.6 fold) in tunica intima
thickness in the DM group compared to the normoglycemic control (p<0.05 ). There was no
significant difference in tunica media thickness and number of elastic lamellae. ln conclusion,
we infer that supplementation with MP modifies early progression or diabetic macrovascular
complication and defers adverse sequalae or diabetes mellitus on aorta. with additional
mechanism beside its anti-hyperglycemic effect. warranting future research. |
| first_indexed | 2024-03-06T16:06:41Z |
| format | Article |
| id | usm.eprints-56996 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T16:06:41Z |
| publishDate | 2018 |
| publisher | Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia |
| record_format | dspace |
| spelling | usm.eprints-569962023-03-01T07:46:47Z http://eprints.usm.my/56996/ Malaysian propolis extract alleviates macrovascular complication in diabetic rats: pharmacological potential of natural bee product Yeoh, Boon Seng R Medicine RM300-666 Drugs and their actions Malaysian propolis (MP) derived from stingless bee: (Heterotrigona itama) possesses antihyperglycemic effect in vivo and show antioxidant activity. The aim of the study was to observe the histological changes in aorta of streptozotocin-induced diabetic rats following MP administration. Thirty adult male Sprague Dawley rats were randomly assigned into five groups (n=6/group) of normoglycemic control (A): diabetic (DM) control (8); DM with metformin (C); DM with MP (D); DM with metformin and MP combination (E). Intraperitoneal streptozotocin (60mg/kg body weight) was employed to induce diabetes mellitus. Distilled water (I ml/day) as vehicle (control). met form in (300mg/kg/day) and MP (300mg/kg/day) were administered orally for four weeks, immediately after successful diabetic induction. The arch of aorta was harvested for histological study using Hematoxylin & Eosin and Verhoeff-Van Gieson stains. Aorta cross sections were examined under light microscope for changes in tunica intima media. Tissues deposition in tunica intima marks the outset of accelerated atherosclerosis in diabetes mellitus. The mean (standard error) or intimal thickness was found to be 2.83 (0.21), 4.52(1.00), 2.68 (0.39), 2.54 (0.36) and 2.11 (0.35) µm for group A, B, C, D and E respectively. This study found a significant increase ( 1.6 fold) in tunica intima thickness in the DM group compared to the normoglycemic control (p<0.05 ). There was no significant difference in tunica media thickness and number of elastic lamellae. ln conclusion, we infer that supplementation with MP modifies early progression or diabetic macrovascular complication and defers adverse sequalae or diabetes mellitus on aorta. with additional mechanism beside its anti-hyperglycemic effect. warranting future research. Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia 2018 Article NonPeerReviewed application/pdf en http://eprints.usm.my/56996/1/DR%20BOON%20SENG%20YEOH-Eprints.pdf Yeoh, Boon Seng (2018) Malaysian propolis extract alleviates macrovascular complication in diabetic rats: pharmacological potential of natural bee product. 34th International Conference on Natural Products 2018. (Submitted) |
| spellingShingle | R Medicine RM300-666 Drugs and their actions Yeoh, Boon Seng Malaysian propolis extract alleviates macrovascular complication in diabetic rats: pharmacological potential of natural bee product |
| title | Malaysian propolis extract alleviates macrovascular complication in diabetic rats: pharmacological potential of natural bee product |
| title_full | Malaysian propolis extract alleviates macrovascular complication in diabetic rats: pharmacological potential of natural bee product |
| title_fullStr | Malaysian propolis extract alleviates macrovascular complication in diabetic rats: pharmacological potential of natural bee product |
| title_full_unstemmed | Malaysian propolis extract alleviates macrovascular complication in diabetic rats: pharmacological potential of natural bee product |
| title_short | Malaysian propolis extract alleviates macrovascular complication in diabetic rats: pharmacological potential of natural bee product |
| title_sort | malaysian propolis extract alleviates macrovascular complication in diabetic rats pharmacological potential of natural bee product |
| topic | R Medicine RM300-666 Drugs and their actions |
| url | http://eprints.usm.my/56996/1/DR%20BOON%20SENG%20YEOH-Eprints.pdf |
| work_keys_str_mv | AT yeohboonseng malaysianpropolisextractalleviatesmacrovascularcomplicationindiabeticratspharmacologicalpotentialofnaturalbeeproduct |