Method validation of delphinidin-3-o-glucoside chloride in rat plasma by High-Performance Liquid Chromatography (HPLC)
Hibiscus sabdariffa (Roselle) is one of the enormously explored medicinal plants with high anthocyanin, which is essential in treating various diseases. This study was conducted on the high-performance liquid chromatography (HPLC) method for detecting and quantifying delphinidin-3-o-glucoside chl...
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| Format: | Thesis |
| Language: | English |
| Published: |
2022
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| Online Access: | http://eprints.usm.my/58375/1/Nur%20Atielah%20Nabila-24%20pages.pdf |
| _version_ | 1797014056696020992 |
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| author | Shafawi, Nur Atielah Nabila Mohd |
| author_facet | Shafawi, Nur Atielah Nabila Mohd |
| author_sort | Shafawi, Nur Atielah Nabila Mohd |
| collection | USM |
| description | Hibiscus sabdariffa (Roselle) is one of the enormously explored medicinal plants with
high anthocyanin, which is essential in treating various diseases. This study was
conducted on the high-performance liquid chromatography (HPLC) method for detecting
and quantifying delphinidin-3-o-glucoside chloride, one of the anthocyanins present in
Hibiscus sabdariffa in the rat plasma. In addition, the HPLC method was validated
following the solid-phase extraction method to demonstrate that this analytical method is
suitable and to assure its reliability of H.sabdariffa pharmacokinetics and bioavailability
study in rat plasma. Six validation parameters were assessed according to FDA guidelines:
linearity, recovery, precision, accuracy, limit of detection, and limit of quantification. The calibration curve of delphinidin-3-0-glucoside chloride had a coefficient determination
(R2) of ≥95.0%. The recovery percentage of delphinidin3-0-glucoside was found to be
80.377%. Delphinidin-3-o-glucoside chloride also showed good precision and accuracy.
The method detection and quantification limits for delphinidin-3-o-glucoside chloride
were 47ng/mL and 213ng/mL, respectively. In conclusion, the validated HPLC method
in this study can be used to determine anthocyanins in the future pharmacokinetic study
of roselle. |
| first_indexed | 2024-03-06T16:10:46Z |
| format | Thesis |
| id | usm.eprints-58375 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T16:10:46Z |
| publishDate | 2022 |
| record_format | dspace |
| spelling | usm.eprints-583752023-05-15T01:59:53Z http://eprints.usm.my/58375/ Method validation of delphinidin-3-o-glucoside chloride in rat plasma by High-Performance Liquid Chromatography (HPLC) Shafawi, Nur Atielah Nabila Mohd QP501-801 Animal biochemistry Hibiscus sabdariffa (Roselle) is one of the enormously explored medicinal plants with high anthocyanin, which is essential in treating various diseases. This study was conducted on the high-performance liquid chromatography (HPLC) method for detecting and quantifying delphinidin-3-o-glucoside chloride, one of the anthocyanins present in Hibiscus sabdariffa in the rat plasma. In addition, the HPLC method was validated following the solid-phase extraction method to demonstrate that this analytical method is suitable and to assure its reliability of H.sabdariffa pharmacokinetics and bioavailability study in rat plasma. Six validation parameters were assessed according to FDA guidelines: linearity, recovery, precision, accuracy, limit of detection, and limit of quantification. The calibration curve of delphinidin-3-0-glucoside chloride had a coefficient determination (R2) of ≥95.0%. The recovery percentage of delphinidin3-0-glucoside was found to be 80.377%. Delphinidin-3-o-glucoside chloride also showed good precision and accuracy. The method detection and quantification limits for delphinidin-3-o-glucoside chloride were 47ng/mL and 213ng/mL, respectively. In conclusion, the validated HPLC method in this study can be used to determine anthocyanins in the future pharmacokinetic study of roselle. 2022-09 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/58375/1/Nur%20Atielah%20Nabila-24%20pages.pdf Shafawi, Nur Atielah Nabila Mohd (2022) Method validation of delphinidin-3-o-glucoside chloride in rat plasma by High-Performance Liquid Chromatography (HPLC). Masters thesis, Universiti Sains Malaysia. |
| spellingShingle | QP501-801 Animal biochemistry Shafawi, Nur Atielah Nabila Mohd Method validation of delphinidin-3-o-glucoside chloride in rat plasma by High-Performance Liquid Chromatography (HPLC) |
| title | Method validation of delphinidin-3-o-glucoside
chloride in rat plasma by
High-Performance Liquid
Chromatography (HPLC) |
| title_full | Method validation of delphinidin-3-o-glucoside
chloride in rat plasma by
High-Performance Liquid
Chromatography (HPLC) |
| title_fullStr | Method validation of delphinidin-3-o-glucoside
chloride in rat plasma by
High-Performance Liquid
Chromatography (HPLC) |
| title_full_unstemmed | Method validation of delphinidin-3-o-glucoside
chloride in rat plasma by
High-Performance Liquid
Chromatography (HPLC) |
| title_short | Method validation of delphinidin-3-o-glucoside
chloride in rat plasma by
High-Performance Liquid
Chromatography (HPLC) |
| title_sort | method validation of delphinidin 3 o glucoside chloride in rat plasma by high performance liquid chromatography hplc |
| topic | QP501-801 Animal biochemistry |
| url | http://eprints.usm.my/58375/1/Nur%20Atielah%20Nabila-24%20pages.pdf |
| work_keys_str_mv | AT shafawinuratielahnabilamohd methodvalidationofdelphinidin3oglucosidechlorideinratplasmabyhighperformanceliquidchromatographyhplc |